Peroral Application of Water-soluble Derivative of Propolis (WSDP) and Its Related Polyphenolic Compounds and Their Influence on Immunological and Antitumour Activity

Polyphenolic compounds are widely distributed in the plant kingdom and display a variety of biological activities, including chemoprevention and growth inhibition of tumours. Propolis contains a conglomerate of polyphenolic compounds. We investigated the effect of propolis and polyphenolic compounds, components of propolis, on the growth and metastatic potential of a transplantable mammary carcinoma (MCa) of the mouse. Metastases in the lung were generated by 2 x 10 tumour cells injected intravenously (i.v.). A water-soluble derivative of propolis (WSDP) and the polyphenolic compounds (caffeic acid (CA) and caffeic acid phenethyl ester (CAPE)) were given to mice perorally before or after tumour cell inoculation. WSDP, CA and CAPE reduced the number of metastases in the lung. This implies that the antitumour activities of the compounds used in these studies are mostly related to the immunomodulatory properties of the compounds, their cytotoxicity to tumour cells, and their ability to induce apoptosis and/or necrosis.     

芦荟粉与蜂胶对肉鸡生产性能和营养物质利用率的影响

选用21日龄AA肉仔鸡公鸡279只,按体重随机分为3组,分别添加120mg/kg的金霉素、600mg/kg的芦荟粉和300mg/kg的蜂胶进行了饲养试验和代谢试验。试验结果表明,芦荟粉和蜂胶的添加显著(P<0.01)提高肉鸡的日增重;分别比抗生素组提高81.99%和64.62%;料重比各组差异显著(P<0.05);代谢试验结果表明:芦荟组和蜂胶组显著提高了(P<0.01)蛋白质和脂肪的利用率,而其余各养分的代谢率各组间均差异不显著(P>0.05)。     

蜂胶醇提物对小鼠学习记忆的促进作用

目的:观察蜂胶醇提物对小鼠学习记忆功能的影响。方法:小鼠灌胃蜂胶醇提物,连续1周,利用Y型迷宫,观察蜂胶醇提物对东莨菪碱所致小鼠记忆获得障碍、亚硝酸钠所致小鼠记忆巩固不良、三氯化铝所致急性衰老小鼠学习记忆障碍的影响。结果:蜂胶醇提物能明显改善东莨菪碱、亚硝酸钠和三氯化铝所致小鼠学习记忆功能障碍。结论:蜂胶醇提物可促进动物的学习记忆功能,可用于防治老年痴呆。     

台湾蜂胶的化学组成与生物活性

本文综合整理了近年关于台湾产蜂胶的研究成果,台湾蜂胶被归类为"太平洋蜂胶",主要可以分成3类,其中以产于5~8月的TW-I台湾绿蜂胶最具生产利用价值。台湾绿蜂胶的化学组成与其它地区产蜂胶的化学组成不同,目前已分离出10种具活性的蜂胶素(propolins),它们属于prenylflavanones类的化合物,我们命名为蜂胶素A、B、C、D、E、F、G、H、I与J。其中蜂胶素A、B、E、H、I与J是新的化合物。在生物活性方面,我们的研究显示台湾绿蜂胶具有多种生物活性功能,包括抗癌、抗氧化与抗菌活性,非常值得进一步研究与利用。     

蜂胶非特异性免疫调节作用的研究进展

综述了国内外蜂胶对非特异性免疫系统的作用及其机理的研究进展,探讨了蜂胶对非特异性免疫反应中各种细胞以及相关免疫介质的影响.研究结果表明:蜂胶能有效提高巨噬细胞、自然杀伤细胞和多形核粒细胞的活性,调节细胞因子的分泌和免疫细胞的增殖,降低巨噬细胞氧化应激反应.     

2013年国内外蜂胶研究概况(一)

对2013年国内外蜂胶的研究概况进行了综述,对公开发表的279篇中英文文献及4篇博士、硕士学位论文的研究领域、地域分布、刊物分布等情况进行了统计分析和评述,并重点介绍了蜂胶的药理活性、化学成分与蜂胶的质量控制、蜂胶的安全性及在农业、畜牧业等方面应用的研究新进展,同时也介绍了2013年国内外蜂胶相关专利的公开情况。     

Effects of Saccharomyces cerevisiae cell wall extract and poplar propolis ethanol extract supplementation on growth performance,digestibility, blood profile,fecal microbiota and fecal noxious gas emissions in growing pigs

A total of 105 growing pigs (24.91 ± 1.06 kg) were used in a 6‐week trial to investigate the effects of including Saccharomyces cerevisiae cell wall extract and poplar propolis ethanol extract (SPE) in the diet on growth performance, digestibility, blood profiles, fecal microbiota and fecal noxious gas emissions. Pigs were randomly allocated to one of three dietary treatments (seven pens/treatment, five pigs/pen) according to initial body weight and sex (two gilts and three barrows). Treatments consisted of a corn soybean meal basal diet supplemented with 0, 0.05 or 0.10% SPE. There was a significant linear improvement (P < 0.05) in average daily gain, gain/feed, the apparent total tract digestibility of dry matter, nitrogen, and gross energy, blood lymphocyte percentage, immunoglobulin G concentration, fecal Escherichia coli and Lactobacillus counts as well as fecal NH₃ and H₂S emissions associated with the inclusion of SPE in the diet. Average daily feed intake, red blood cells and white blood cells concentration were not significantly (P > 0.05) affected by SPE supplementation in the diets. In conclusion, results indicate that dietary SPE supplementation can improve growth performance, digestibility and fecal microbiota, and decrease fecal gas emissions in growing pigs.     

蜂胶对糖尿病SD大鼠的影响

测定和比较了蜂胶醇提液(EEP)和蜂胶水提液(WSP)对糖尿病SD大鼠的影响. 结果表明,EEP和WSP均能降低糖尿病SD大鼠的空腹血糖(FBG)、糖化血红蛋白(HBAlc)、果糖胺(FRU)、甘油三酯(TG)、总胆固醇(TC)、肌酐(CREA)、尿素氮(BUN)、尿酸(UA)、丙二醛(MDA)水平,减少总蛋白(TP)和白蛋白(ALB)的消耗,降低肾重与体重的比值. 表明蜂胶能够改善糖尿病SD大鼠的糖、脂肪、蛋白质代谢,减少糖尿病肾病的危害.     

兔病毒性出血症、巴氏杆菌病二联蜂胶灭活苗的研制

应用兔出血症病毒(RHDV)和巴氏杆菌(Pm)联合研制而成的蜂胶佐剂灭活苗,用 1 mL 免疫试验兔,免疫后第 5d,对 RHDV 保护率达 100%(5/5);免疫后第 14d,对巴氏杆菌的保护率达 100%(4/4),第 7d 即产生较强的免疫力。T 细胞总玫瑰花环形成率(Et率)、活性玫瑰花环形成率(Ea率)的测定结果表明:蜂胶具有提高机体细胞免疫功能的作用;通过 HI 法监测兔病毒性出血症抗体水平,结果表明:蜂胶苗产生抗体时间较早,第 7d 抗体效价可达到较高水平(26.8),第 21 d 达到高峰(28.8)。     

Study of Chemical Composition and Efficacy of Different Extracts of Iranian Propolis on the Microbiological and Sensory Parameters of Minced Cyprinus carpio Meat at 4°C Storage

This study has evaluated the effects of different concentrations of the water and ethanolic extracts of Iranian propolis (WEP and EEP) on the microbiological and sensory parameters of minced common carp meat that was stored at 4°C for 9 days. The chemical compounds of the extracts were identified by gas chromatography–mass spectroscopy (GC-MS) analysis. The results revealed that WEP and EEP extracts were efficient against the spoilage microorganisms, including total plate counts (TPCs), psychrotrophic populations (PTCs), lactic acid bacteria (LAB), and even Staphylococcus aureus. There was a significant difference in the bacteria growth between samples treated with WEP or EEP and the control sample during storage. Inhibitory effects were, respectively, 7% EEP > 5% EEP > 3% EEP > 7% WEP > 5% WEP > 3% WEP. In all treatments, PTC counts were higher than 7 log CFU/g after 6 days as well as TPC, LAB, and S. aureus populations after 9 days. Overall, the results show that EEP and WEP have good antimicrobial activity due to high phenolic and flavonoid compounds, including caffeic acid, tectochrysin, aceacetin, chrysin, pinostrobin, pinobanksin-3-acetate, galangin-3-methyl ester, and pinocembrin chalcone. Sensory evaluation shows very slight improvement in sensory properties (color, odor, texture, and overall acceptability) of the treated samples with the different extracts of Iranian propolis.