鸡传染性贫血病毒VP1、VP2基因在杆状病毒表达系统中的表达

将鸡传染性贫血病毒M9905株VP1、VP2基因分别或同时克隆到杆状病毒转移载体pFastBacDUAL中，然后转化到DHIOBAC感受态细胞中与Bacmid杆状病毒穿梭载体进行转座重组，最后将重组子转染Sf9昆虫细胞，得到分别或同时含VP1、VP2基因的重组杆状病毒rBacVP1、rBacVP2及rBacVP1—2。PCR扩增结果证实VP1、VP2基因重组到杆状病毒基因组中；SDS-PAGE电泳分析和间接免疫荧光试验结果表明VP1、VP2基因在重组病毒中得到了表达。     

NO对大鼠中脑腹侧被盖区DA神经元的调节

实验观察了微量注射 NOS抑制剂 L -NAME及微量联合注射 NO的前体 L-精氨酸( L-Arg) L-NAME于大鼠中脑腹侧被盖区( VTA)对该部位多巴胺神经元的调节。发现VTA注射 L -NAME( 1 mg/ 5μL )后 ,伏隔核( Acb)多巴胺 ( DA )代谢产物—双羟苯乙酸( DOPAC)水平升高到注射前的 1 2 2 .5% ( P <0 .0 0 1 ) ,小剂量注射 L-NAME( 0 .2 mg/ 5μL)对伏隔核 DOPAC水平无明显影响 ;同样方法联合注射 L-Arg( 3 0 0 μg/ 5μL) L-NAME( 1 mg/5μL)后 ,伏隔核 DOPAC水平无明显变化。结论 :VTA微量注射 L-NAME兴奋了该部位的DA神经元 ,而 L -Arg L -NAME联合注射 ,却不能影响 DA神经元的活动 ,说明 NO可以通过L-Arg-NOS-NO途径参与 VTA多巴胺神经元的调节     

Impact of postovulatory food deprivation on the ova transport, hormonal profiles and metabolic changes in sows.

The effect of food deprivation on ova transport, hormonal profiles and metabolic changes was studied in 20 crossbred multiparous sows during their second oestrus after weaning. To determine the time of ovulation, transrectal ultrasonographic examination was performed. The sows were divided into 2 groups, one control group (C-group), which was fed according to Swedish standards, and one experimental group (E-group). The E-group sows were deprived of food from the first morning meal after ovulation until slaughter. Blood samples were collected every second hour from about 12 h before expected ovulation in the second oestrus after weaning until slaughter and were analysed for progesterone, prostaglandin F2 alpha-metabolite, insulin, glucose, free fatty acids and triglycerides. All sows were slaughtered approximately 48 h after ovulation and the genital tract was recovered. The isthmic part of the oviduct was divided into 3 equally long segments and flushed separately with phosphate buffered saline (PBS). Uterine horns were also flushed with PBS. A significantly greater number of ova were found in the first and second part of the isthmus in the E-group (p = 0.05) while in the C-group most of the ova were found in the third part of the isthmus or the uterus (p = 0.01). The level of prostaglandin F2 alpha-metabolite was significantly higher in the E-group compared with the C-group. The concentration of progesterone increased in both groups after ovulation but there were no significant differences between the groups. The other blood parameters showed that the food-deprived sows were in a catabolic state. The 48 h period of fasting results, directly or indirectly in an delayed ova transport, which may be due to a delayed relaxation in the smooth circular muscle layer of the isthmus.     

抗虫杂交棉F1、F2代纤维品质的比较研究

2003年,在山东尹庄、江苏扬州、安徽宿松、湖北荆州、湖南长沙、湖南南县、湖南澧县等7个地点,对包括对照中29在内8个组合的F1、F2代共计16个材料进行纤维品质的比较研究,结果表明:抗虫杂交棉F1和F2代间除纤维长度达到显著差异外,其他7项指标差异均无显著,但F2代纤维品质变异范围增大,劣质纤维比例增加,优良纤维比例减少,从而出现纤维品质的衰退。     

线粒体与细胞凋亡

细胞凋亡是一种由基因控制的细胞自杀性死亡过程,是机体维持自身稳定的一种基本生理机制。机体通过细胞凋亡清除损伤、衰老与突变的细胞,维持生理平衡。通过对细胞凋亡的研究发现,线粒体在调节细胞凋亡中发挥着关键作用。尤其是被称为细胞凋亡主开关的线粒体通透性改变孔(MitochondrionPermeabilitytransitionPore,MPTP),它是细胞色素C、Smac、AIF等凋亡诱导因子的主要来源。本文以MPTP的开放、细胞色素C、AIF在细胞凋亡中的作用以及Bcl-2家族对细胞凋亡的调控做一综述,以便广大读者能系统地了解线粒体在细胞凋亡中的作用。     

The Phytotoxicity of Various Graminicides in Durum Wheat in Greece

The effect of three herbicides, diclofop-methyl (DM), imazamethabenz-methyl (IM) and fenoxaprop-ethyl (FE), was investigated at the recommended rate (×) and double the recommended rate (2×) in a durum wheat crop. FE had the smallest effect on root system characteristics, while IM had the greatest effect. For all herbicides, the double rate treatment gave a greater statistical reduction than the single rate for root system characteristics. FE had the smallest effect on the activity of aerobic microorganisms, while DM gave the greatest reduction, 10 days after application, and all three herbicides reduced earthworm abundance. DM gave the smallest reduction in the number of tillers and in plant height, while the double rate of IM gave the greatest reduction. For all three herbicides, double the recommended rate resulted in a significant reduction in the weight of 1000 seeds, while no statistically significant deviations were noted at the recommended rate. Finally, the use of all three herbicides resulted in significantly lower statistical yields compared to the sample (control plot), although there were no significant statistical differences among them, for either recommended or double recommended rates.     

牛奶葡萄贮藏中SO_2伤害及调控措施研究

牛奶葡萄采后SO2双重释放装置辅以CT－1处理不但有效降低了药害发生率，而且提高了贮藏效果。在此基础上辅以采前喷布内含杀菌剂的壳多糖，牛奶葡萄可达到更佳贮藏效果，在1998年生长季发生全国性洪水灾害，田间带菌量剧增，各地葡萄贮藏效果极差情况下，参试的牛奶葡萄腐烂率只有1．6％，果梗鲜绿，褐变指数只有0．4％，药害率仅为1．0％，果肉亚硫酸盐残留仅为0．067 mg／kg。     

CO_2施肥对大棚油桃光合作用及产量品质的影响

棚内CO2 浓度日变化幅度远大于棚外 ;在天气晴好、光合旺盛、通风受阻时 ,棚内CO2 匮乏可成为光合作用的主要限制因子。在保护地条件下 ,油桃光合速率 (Pn)日变化由露地的双峰曲线变为三峰曲线 ,最大Pn比露地提前 2h ,在 8时左右出现 ;光合“午休”现象不明显 ;晴天棚内日平均Pn为 5 .97(CO2 ) μmol/m2 ·s,比露地降低17.2 5 %。棚内CO2 加富对油桃光合作用和产量、品质有重要影响 ,较大幅度地提高了上午 8时～ 12时之间的Pn和光能利用率 ,日平均Pn 6 .95 (CO2 ) μmol/m2 ·s ,比对照提高 2 5 .90 %。树体生长健壮 ,生物量 (未含果实 )增加 12 .4% ,比叶重增大 ,产量提高 19.80 % ,品质改善     

焦锑酸钾沉淀法定位Ca~(2+)在梨生理研究中的应用

介绍了应用焦锑酸钾沉淀法定位Ca2＋的方法，以及在梨生理研究中的具体步骤和应用效果。结果表明：在固定液中加焦锑酸钾处理进行制样，梨树不同器官或组织细胞中的自由态Ca2＋，都能生成焦锑酸钙在原部位沉淀，经染色后在电子显微镜下呈现出黑色颗粒，而固定液中不加焦锑酸钾处理的则没有这种颗粒。再进一步用Ca2＋的专一性鳌合物EGTA进行处理，检查这些黑色颗粒是否消失，以证实黑色颗粒就是Ca2＋，从而可对Ca2＋进行定位标记。在不同的组织细胞及同一组织细胞在不同的生理时期，其被标记的Ca2＋密度和场所是不同的，可通过其动态变化来探讨它的作用机理。还对用此方法定位Ca2＋的注意事项进行了讨论。     

O,O-二烷基-N-莰-2-基(硫)磷酰胺酯的合成

合成了14个含有 N-莰-2-基的磷酰胺和硫代磷酰胺类化合物,用红外、核磁及元素分析确定其结构,并探讨了~(31)Pδ值的规律性变化。初步生物试验表明,磷酰胺类化合物对水稻发芽具有明显的抑制作用,其中化合物Ⅱ1的活性大于对照药剂抗倒胺但低于多效唑。