基于感知和行为视角的校园滨水景观偏好研究 ——以福建农林大学观音湖为例

基于人群感知和行为视角,以福建农林大学观音湖为研究对象,通过拍照试验、访谈问卷、扫描观察收集数据,采用词频分析、照片分析、GIS技术分析,分析景观元素、景观感知以及驻留点、观景点、景点、视线廊道的空间特征,以获得游览者对滨水景观偏好特征,采用核密度分析,得到游客景观点的热点分布。结果表明,词频分析预测出游客关注湖区丰富的景观元素,以静态观赏和行进式观赏方式游览观音湖,舒适的湖区环境让游客感到心情愉悦、平静;空间角度,游览者的驻留点分布与观景点分布具有显著的一致性,表明优美的空间环境和良好的观景位置有利于游客驻留行为的产生;游览者对滨水景观的偏好是由“丰富性—功能性—特色性”三大属性特征相互协调、影响而形成的三维耦合结构模型。     

非热处理对蜂花粉杀菌效果及品质的影响

为探究更有利于保留蜂花粉原有营养和风味的杀菌方法,本研究分别用辐照、超高压和高压静电场3种非热杀菌技术处理蜂花粉,并测定营养及风味的指标。结果表明,7 kGy辐照、500 MPa超高压、30 kV高场强处理对蜂花粉中的微生物均有抑制作用,且辐照对细菌的抑制效果较好,超高压和高压静电场对真菌的抑制效果较好。经500 MPa超高压处理后,蜂花粉脂类含量增加了23.3%,酚酸等活性成分含量增加,且其抗氧化活性降低程度最小,颜色和风味最接近未处理蜂花粉;30 kV高场强处理后,蜂花粉抗氧化活性显著下降、颜色偏深偏暗;7 kGy辐照处理后产生了明显的辐照味。综上,蜂花粉经超高压灭菌能达到较好的杀菌效果,而且能最大限度保持蜂花粉原有品质。本研究为非热杀菌技术在蜂花粉加工产业中的应用提供了理论依据。     

硅钙钾镁肥配施黄腐酸钾对土壤酶活性及桃幼树生长的影响

为探究硅钙钾镁肥配施黄腐酸钾对桃树生长的效应,以盆栽一年生瑞蟠21号毛桃(Amygdalus persica Linn.)为试验材料,设置3种不同施肥方式(T1:硅钙钾镁;T2:硅钙钾镁+黄腐酸钾;CK:空白对照),在桃幼树生长季进行追肥处理,研究硅钙钾镁肥及硅钙钾镁肥配施黄腐酸钾对土壤酶活性、土壤养分状况及桃幼树根系构型和植株生长的影响。结果表明,与CK相比,T1、T2均显著提高了桃幼树生长季各时期土壤酸性磷酸酶、脲酶和过氧化氢酶活性,以及土壤中碱解氮、有效磷、速效钾和有效硅等含量,且配施黄腐酸钾后作用更明显。与CK相比,T1、T2细根比重分别提高了11.2%和26.2%,根系活力分别提高了12.2%和18.1%,根系总长度分别增加了28.1%和61.9%,且有效延缓了根系衰老;T1、T2植株总干重分别提高了1.2倍和2.0倍,根冠比分别提高了9.4%和17.0%,且差异显著。与CK相比,T1、T2各器官中全氮、全磷、全钾含量均显著增加,地上部硅含量分别提高了61.3%和91.9%,地下部硅含量分别提高了62.3%和102.5%,且差异显著。同时,T1、T2桃幼树叶片叶绿素含量和净光合速率均显著提高。综上所述,硅钙钾镁肥可有效提高土壤酶活,促进桃幼树细根的生长,延缓根系衰老进程,增加氮、磷、钾、硅等营养元素积累量,提高叶片光合速率,促进植株生长;硅钙钾镁肥配施黄腐酸钾后,提高了土壤有效硅及植株硅含量,比单施硅钙钾镁肥效果更加显著。本研究结果为桃树合理施用硅钙钾镁肥提供了理论依据。     

60Co-γ射线和电子束辐照灭菌对葛根提取物抗氧化活性及指纹图谱的影响

为评价⁶⁰Co-γ射线和电子束辐照灭菌对葛根提取物品质的影响效应及其差异性,采用不同剂量的⁶⁰Co-γ射线(0、2.8、5.7、8.3、10.6 kGy)和电子束(0、2.4、5.1、7.7、10.3 kGy)辐照处理葛根提取物,研究辐照对其微生物总数、抗氧化活性及指纹图谱的影响。结果表明,⁶⁰Co-γ射线和电子束辐照均能有效降低葛根提取物中需氧菌总数、霉菌和酵母菌总数,随着吸收剂量增大,抑制作用增强。⁶⁰Co-γ射线和电子束辐照吸收剂量分别为5.7 kGy和5.1 kGy时,葛根提取物均达到《中国药典》所规定的微生物限度标准(需氧菌总数为10³ CFU·g^-1、霉菌和酵母菌总数为10² CFU·g^-1);吸收剂量分别为5.7 kGy和7.7 kGy时,需氧菌总数、霉菌和酵母菌总数均降至10 CFU·g^-1以下。此外,大肠埃希氏菌(Escherichia coli)与沙门氏菌(Salmonella)均未检出。2种辐照方式均对葛根提取物的DPPH自由基(DPPH·)清除率、羟自由基(·OH)清除率以及总还原能力无显著影响(P>0.05)。不同剂量⁶⁰Co-γ射线和电子束辐照处理葛根提取物特征峰的相似度达到1.0,且相对保留时间的相对标准差(RSD)均小于0.5%,表明2种辐照方式对葛根提取物指纹图谱均无显著影响(P>0.05)。综上所述,⁶⁰Co-γ射线和电子束辐照对葛根提取物微生物具有较强的抑制作用,2种辐照方式均不会对葛根提取物的抗氧化活性及指纹图谱产生显著影响,不影响葛根提取物的有效性及质量稳定性。该研究为2种辐照技术,特别是电子束辐照在葛根药材、饮片及其中成药制剂灭菌中的合理应用提供了科学参考。     

黄河未来输沙量态势及其适用性对策

[目的] 探索黄河输沙预测的新思路，预估黄河未来输沙态势与输沙量水平，为黄河流域生态治理规划提供参考。[方法] 结合黄河流域水土保持生态修复现状，采用单累积曲线法、滑动平均及频率分析方法，分析1950—2019年黄河主要来沙区间的实测输沙量变化特征及其未来态势。[结果] 1950—2019年黄河输沙量呈现阶梯式减少。1950—2019年黄河中游各站累积实测输沙量随时间的变化可用“左半抛物线”表征。黄河输沙量自1997年以来已进入相对稳定态势，目前已达企稳状态；黄河潼关站未来年输沙量在90%频率下为1.00×10⁸ t左右，在10%频率下为5.00×10⁸ t左右，未来多年平均输沙量为1.40×10⁸ t。[结论] 为了维持黄河输沙量低稳状态，提升水土保持措施质量与标准，补齐“后水土保持”短板，构建完善的水沙关系调控体系，维持黄河下游河道冲淤平衡，是黄河流域生态保护与高质量发展的保障。     

海南粗榧内生真菌F127发酵液的次级代谢产物研究

对海南粗榧(Cephalotaxus hainanensis Li.)内生真菌F127发酵液化学组分进行研究。用多种色谱技术对F127代谢产物进行分离纯化,以LC-MS、ESI-MS和超导核磁共振分析鉴定化合物结构,最后采用MTT法测定化合物的抗肿瘤活性。从海南粗榧内生真菌F127的发酵液中分离得到5个单体化合物,分别鉴定为oblongolide T(1)、sorbicillin(2)、邻苯二甲酸二丁酯(3)、phomopsolide B(4)、6,8-二羟基-3-甲基-3,4-二氢异香豆素(5),且化合物1、4、5对肿瘤细胞K562、NB4、HL-60、Hep G-2和Lovo表现出不同抑制活性。化合物4对5株肿瘤细胞均表现出抑制活性,对K562、NB4、HL-60抑制效果显著,IC50值分别为3.35、0.014和0.16μg/m L,对Hep G-2和Lo Vo只有温和抑制作用;化合物1对K562、NB4、HL-60抑制作用良好,IC50值分别为51.82、54.25和29.31μg/m L;化合物5对NB4和Hep G-2则具有一定抑制活性;化合物2和3对测试细胞株未表现出抑制活性。5个化合物均是首次从海南粗榧内生真菌中分离得到,并首次报道了化合物1、4对K562、NB4、HL-60的优良细胞毒活性,为进一步研究海南粗榧内生真菌中的活性天然产物奠定了基础。     

Characteristics of starch synthesis and grain filling of common buckwheat

The common buckwheat ‘cv. Fengtian 1’ (FT1), ‘cv. Yuqiao 4’ (YQ4), ‘cv. Dingtian 2 (DT2)’, and ‘cv. Tongliao (TL)’ were selected to investigate the characteristics of starch synthesis and grain filling. The chlorophyll and chlorophyll a/b contents of leaves in the third stem showed a steady downward trend with the growth of common buckwheat. Whereas, FT1, YQ4 and DT2 showed higher chlorophyll content than that of TL at 7d and 14d. The activity of soluble starch synthase(SSS) and adenosine diphosphate glucose pyrophosphorylase (ADPGP) in the four tested varieties showed a rapid upward trend until 14 days after heading but then rapidly decreased until 21 days after heading. The average adenosine diphosphate glucose pyrophosphorylase activities of FT1, YQ4 and DT2 were higher than TL, while the average soluble starch synthase activities were similar among the four tested varieties. Consistently, the four tested varieties showed rapid accumulation of starch, amylose, and amylopectin during the prefilling stage, but no difference of starch content was found at maturity. The filling process of the four common buckwheat varieties exhibited an “S” curve. The Richards equation was utilized to evaluate the grain-filling process of common buckwheat. YQ4 showed the largest values of initial growth power (R₀). The time reaching the maximum grain-filling rate (t_max.G) of FT1 was the longest, and the maximum grain-filling rate (G_max) of DT2 was the fastest.     

果蔬籽油生物活性研究进展

果蔬籽油是由果蔬籽经压榨或萃取工艺制得的植物油脂。果蔬籽油含有丰富的维生素、多不饱和脂肪酸、有机酸等活性成分,这些成分赋予了果蔬籽油抗氧化、抗癌、调节血脂和调节免疫系统等生物活性。本文综述了果蔬籽油生物活性的研究进展,旨在为果蔬籽油的综合开发和利用提供理论依据和指导。     

HXKs基因在香蕉果实后熟过程中功能初探

香蕉是世界主要水果之一,对乙烯非常敏感,属于呼吸跃变型果实。目前,香蕉果实后熟机理还未完全探明,后熟过程中己糖激酶(HXK)与乙烯的关系还不清楚。通过BLAST比对从香蕉基因组数据库中发现HXK基因家族的11个成员,经克隆获得这些基因的序列,并研究HXKs基因在香蕉果实自然后熟过程中的转录表达谱,重点研究乙烯利、甘露糖、NAG和1-MCP对HXKs基因表达、HXK酶活和内源乙烯生物合成的影响。结果表明:在香蕉果实后熟过程中HXKs基因呈现差异性表达,乙烯利上调大多数HXKs基因的表达和HXK酶活,1-MCP的作用正好相反,这表明外源乙烯正作用于HXK。另一方面,甘露糖加快内源乙烯生物合成,NAG却推迟内源乙烯生物合成,这说明HXK正作用于内源乙烯生物合成。所以,在香蕉果实后熟过程中乙烯和HXK之间存在相互促进的关系。这将为进一步阐明香蕉果实后熟机制提供理论依据,也将为香蕉果实保鲜新技术的挖掘提供新思路。     

Resistance monitoring and enzyme activity in three field populations of cowpea aphid (Aphis craccivora) from Egypt

The cowpea aphid (Aphis craccivora) is one of the most important sucking insect pests attacking certain legumes in Egypt particularly faba bean, cowpea and pea. In this study we monitored the resistance level of three field populations of A. craccivora to seven insecticides belonging to three different chemical classes (organophosphates, carbamates and neonicotinoids). The three populations were collected from three governorates in Egypt namely Dakahlia, Qalyobia and Beni Suef. Diagnostic concentrations (LC₉₀ values for susceptible strain) for each insecticide were established using a leaf dipping technique. Resistance monitoring showed that the field population from Dakahlia was highly resistant to all the tested insecticides. In a similar manner, the population from Qalyobia was also resistant to all insecticides except for fenitrothion to which only moderate resistance was observed. The field population from Beni Suef exhibited a lower level of resistance to all the seven tested insecticides.Biochemical assay showed that esterase activity in these three field populations was generally higher as the enzyme activity ratio ranged from 4.3 to 7.8 fold more than that for the susceptible strain. The activity of the other measured detoxifying enzymes (glutathione -S- transferase and mixed function oxidases) was moderate in the populations from Qalyobia and Dakahlia. Nevertheless, the enzyme activity in A. craccivora collected from Beni Suef was variable and differed slightly from the activity measured in the susceptible strain. Monitoring insecticide resistance among the three aphid populations was a proactive approach to detect any shift in insecticide efficiency. The possible occurrence of resistance in the cowpea aphid to the tested insecticides may be due to the higher activity of carboxylesterases. Further studies on the resistance mechanism to these insecticides are needed to provide insights in how to manage and delay the onset of the resistance and thus prolong the performance of insecticides against A. craccivora.