Leaf carbohydrates assimilation and metabolism affect seed yield of rapeseed with different waterlogging tolerance under the interactive effects of nitrogen and waterlogging

Waterlogging is a main factor causing rapeseed (Brassica napus L.) yield loss, and reasonable nitrogen (N) applications can compensate for this loss. To investigate the effects of N rates on seed yield of waterlogged rapeseed, the waterlogging-tolerant rapeseed variety ZS 9 and sensitive variety GH01 were waterlogged for 0 and 10 days with five leaves at the seedling stage under four N rates (0, 90, 180 and 270 kg/ha). Waterlogging significantly decreased seed yield, while N application can alleviate the yield loss. The yield decrease rate of waterlogged GH01 was greater than that of ZS 9 under the same N rate. During the seedling and bolting stage, the leaf photosynthetic rate (Pn) and ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) activity increased, while activities of adenosine diphosphate glucose pyrophosphorylase (AGPase), sucrose synthase (SuSy) and sucrose phosphate synthase (SPS) decreased with more N under the same watering conditions. Compared to the plants without waterlogging, the leaf Pn and Rubisco activity, starch and sucrose contents of waterlogged rapeseed decreased at the two stages; activities of AGPase, SuSy and SPS of waterlogged rapeseed decreased at the seedling while increased at the bolting stage for both the two varieties. At the flowering stage, the Pn, the activities of Rubisco, AGPase, SuSy, SPS and contents of sucrose, starch increased with more N application for both ZS 9 and GH01. Compared to the plants without waterlogging, the Pn and Rubisco activity for waterlogged plants of the two varieties increased; the waterlogged plants of tolerant variety had higher activities of AGPase, SuSy and SPS, while those of sensitive variety was significantly lower. However, the decreased starch and sucrose content were found in both tolerant and sensitive varieties. The activities of AGPase, SuSy and SPS at flowering were highly positively correlated with yield under the interactive effects of N and waterlogging. These results suggested that the flowering stage is the most important stage that N had the positive regulation on waterlogged rapeseed growth. The carbohydrates translocation from leaves to seeds of the tolerant variety were enhanced after waterlogging, while that of the sensitive variety was still inhibited. This was the main reason for the difference in yield between the two waterlogged varieties.     

Engineered-airlift pumps for aquaculture applications using multiphase flow analysis

Currently, very few aquaculture operations are employing airlift pump technology for water recirculation, aeration, and waste removal. This is likely due to the poor design and lower efficiency of traditional airlift design, the limited amount of research effort that has been invested in improving performance capabilities of air lift pumps and the general lack of awareness of the industry about the inherent advantages of airlift systems. A new efficient airlift pump is hydrodynamically designed by incorporating the Volume of Fluid (VOF) multi-phase model along with the K-ε turbulence model utilizing Computational Fluid Dynamics (CFD) tools. The pump is designed to offer a substantial reduction in total energy usage as well as an improved quality of the culture products in order to make it attractive to aquaculture industry. In this study, both numerical and experimental investigations were carried out for airlift systems operating under two different submergence ratios of 50% and 90% in a lab setting using 2.54 cm diameter pumps. Also, the performance of a large-scale pumps of 10.16 cm diameters were also tested in an aquaculture raceway to determine its effect on the operation. The numerical results were found to be in agreement with the experiments within ±20% which is considered very reasonable for multiphase flow analysis. The present study was found to present a great tool for modelling the airlift pump performance, and potentially proposing new designs.     

枣实蝇对枣果挥发物的选择行为

[目的]探究对枣实蝇有引诱作用的挥发性物质,研发基于行为调节的防治技术。[方法]采用Y型嗅觉仪测定了枣实蝇成虫对6种枣果挥发物的选择行为反应,并在室内试验的基础上进行田间引诱试验。[结果]室内试验结果显示,枣实蝇成虫对6种枣果挥发物具有显著正趋性。枣实蝇雌虫对棕榈酸乙酯、月桂酸乙酯、肉豆蔻酸的选择率较强,选择率均为30%以上;雄虫对亚油酸乙酯、油酸乙酯的选择率较强,选择率均为25%以上,从不同浓度梯度(原液、10%溶液、1%溶液)的选择率和选择系数来看,随浓度的递减,其选择率和选择系数也在递减,原液的选择率分别为10%溶液及1%溶液的2.5倍及5倍以上。田间试验发现:肉豆蔻酸对枣实蝇雌虫的诱集效果较强,2 d最多诱捕数量为15头·只^-1,平均诱捕数量为11.4头·只^-1,十四酸乙酯次之。在6种不同挥发物中油酸乙酯和亚油酸乙酯对枣实蝇雄虫的引诱效果较强。经单因素方差分析发现,在6种挥发性物质及混合物中,月桂酸乙酯及十四酸乙酯对枣实蝇雌雄虫的引诱效果之间存在显著性差异。其他挥发物之间无显著差异。[结论]枣果挥发物中棕榈酸乙酯、月桂酸乙酯、肉豆蔻酸与枣实蝇偏爱选择密切相关。在田间试验中:枣实蝇成虫对肉豆蔻酸的选择率较强,为研发枣实蝇引诱剂提供了理论依据。     

羊角椒新品种寿研梦扬的选育

寿研梦扬是由母本CSVHPM1407001和父本CSVHPF1407002配制而成的羊角椒一代杂种。植株长势旺盛,连续坐果能力强;果实长羊角形,果长25～30 cm,果肩宽3～4 cm;青熟果黄绿色,老熟果红色;外表光亮,商品性好,耐贮运;单果质量100 g左右,辣味浓,宜鲜食;田间对病毒病、炭疽病和疫病的抗性强于对照喜洋洋。保护地栽培鲜椒产量可达12 840 kg·(667 m~2)~(-1)左右。适宜山东、河北等地区早春、秋延保护地种植。     

含刚性沉水植物明渠水流结构的试验研究

【目的】得到含刚性沉水植物明渠的水流结构。【方法】采用粒子图像测速仪(PIV),用有机玻璃棒模拟刚性植物,在不同来水流量、植物密度条件下对明渠水流结构进行了试验研究。【结果】无植物时平均流速沿垂向呈对数分布规律,有植物时则呈现明显的分区分布特性。无植物时紊动强度值沿垂向变化不大,有植物时在植物顶端位置处紊动最剧烈。流量或植物密度越大,植物顶端位置的流速梯度就越大,植物层上方的流速最大值也越大。植物密度越大,植物顶端位置的紊动强度越大,植物密度对水流紊动强度由最大值减小到最小值的区域影响很大。【结论】刚性沉水植物的存在会改变水流结构,增强紊动掺混,增强流体质点交换和能量传递,且上述影响会随着流量或植物密度的增大而增强。     

耐盐抗旱优质高产棉花新品种‘衡棉1670’的选育研究

抗逆优质丰产棉花新品种的培育及应用是当前棉花生产节水提质增效的关键。‘衡棉1670’是河北省农林科学院旱作农业研究所以中早熟陆地棉品种资源材料‘衡棉210’为母本,以‘衡棉4号’为父本杂交,后代多年南繁北育,经旱棚盐池鉴定选择,在枯黄萎病混生重病地、不防治棉铃虫的高压胁迫下,经过多年连续定向选择育成。其突出表现为抗枯黄萎病、丰产优质、耐盐抗旱节水,适宜在河北省及黄河流域同类生态春播棉区推广种植,2019年8月通过河北省农作物品种审定委员会审定（审定编号为冀审棉20190013）。     

粪肠球菌生长曲线的测定及其对小鼠脑组织的影响

为测定致羔羊脑炎粪肠球菌（Enterococcus faecalis）的生长曲线，寻求一种快速而准确的方法测定不同生长时期粪肠球菌数量，并客观评价其毒性强弱及其对小鼠脑组织的影响，试验采用平板菌落计数法和OD-Monitor振荡比浊法（D_λ值法）测定粪肠球菌的生长曲线，探究该菌在合适时间段内的吸光值（D_{600 nm}）与平板菌落计数法测定的活菌数（CFU）的关系。用粪肠球菌感染小鼠，观察记录小鼠的死亡情况，最后采用Karber法计算粪肠球菌感染小鼠的半数致死量（LD₅₀）。用LD₅₀的剂量感染小鼠，及时采集死亡小鼠脑组织，未死亡的小鼠72 h后全部剖杀取脑组织，一部分做涂片染色，制作病理切片，观察病理变化；一部分进行培养，用于PCR方法进行细菌的回收鉴定。结果显示，用两种方法测定此株粪肠球菌的生长曲线基本一致，在2~8 h生长迅速，为对数生长期，8~14 h生长缓慢，为稳定期，14 h之后死亡数增加，进入衰亡期；对12 h粪肠球菌D_{600 nm}与CFU的关系进行探讨，成功建立回归方程：y=20.769x-1.3422，R²=0.997；其感染小鼠的LD₅₀为7.77×10¹¹个活菌。以此剂量感染小鼠，脑组织涂片染色和培养染色，均能看到革兰氏阳性球菌；PCR结果显示，均出现了大小为112 bp的条带。对脑组织进行病理学观察发现该菌可导致脑组织充血、出血、形成微血栓，脑膜充血。通过生长曲线和其D_{600 nm}与CFU关系的建立，可实时监测粪肠球菌数量，为后期更深入研究粪肠球菌穿越血脑屏障的机制奠定重要的理论基础。     

低聚半乳糖干预缓解肠道炎症的研究进展

肠道炎症已成为我国社会健康的难题和挑战，其发病率在我国迅速增长。肠道炎症发病原因复杂，目前尚缺乏有效的缓解药物，因此加强肠道炎症有效缓解物质的研发至关重要。低聚半乳糖（galactooligosaccharide，GOS）是一种食疗益生性较优的乳源功能性低聚糖，能够有效促进肠道内益生菌的增殖，改变肠道菌群结构，刺激免疫应答，进而改善肠黏膜屏障功能，缓解肠道炎症。本文综述近年来国内外有关肠道炎症及GOS干预缓解肠道炎症作用的研究进展，并对其应用前景进行展望，为此领域的研究提供科学依据。     

Regulatory role of l-proline in fetal pig growth and intestinal epithelial cell proliferation

l-proline (Pro) is a precursor of ornithine, which is converted into polyamines via ornithine decarboxylase (ODC). Polyamines plays a key role in the proliferation of intestinal epithelial cells. The study investigated the effect of Pro on polyamine metabolism and cell proliferation on porcine enterocytes in vivo and in vitro. Twenty-four Huanjiang mini-pigs were randomly assigned into 1 of 3 groups and fed a basal diet that contained 0.77% alanine (Ala, iso-nitrogenous control), 1% Pro or 1% Pro + 0.0167% α-difluoromethylornithine (DFMO) from d 15 to 70 of gestation. The fetal body weight and number of fetuses per litter were determined, and the small and large intestines were obtained on d 70 ± 1.78 of gestation. The in vitro study was performed in intestinal porcine epithelial (IPEC-J2) cells cultured in Dulbecco''s modified Eagle medium-high glucose (DMEM-H) containing 0 μmol/L Pro, 400 μmol/L Pro, or 400 μmol/L Pro + 10 mmol/L DFMO for 4 d. The results showed that maternal dietary supplementation with 1% Pro increased fetal weight; the protein and DNA concentrations of the fetal small intestine; and mRNA levels for potassium voltage-gated channel, shaker-related subfamily, member 1 (Kv1.1) in the fetal small and large intestines (P < 0.05). Supplementing Pro to either gilts or IPEC-J2 cells increased ODC protein abundances and polyamine concentrations in the fetal intestines and IPEC-J2 cells (P < 0.05). In comparison with the Pro group, the combined administration of Pro and DFMO reduced the expression of ODC protein and spermine concentration in the fetal intestine, as well as the concentrations of putrescine, spermidine and spermine in IPEC-J2 cells (P < 0.05). Meanwhile, the percentage of cells in the S-phase and the mRNA levels of proto-oncogenes c-fos and c-myc were increased in response to Pro supplementation, whereas depletion of cellular polyamines with DFMO increased tumor protein p53 (p53) mRNA levels (P < 0.05). Taken together, dietary supplementation with Pro improved fetal pig growth and intestinal epithelial cell proliferation via enhancing polyamine synthesis.