Meta-Analysis of 100-Seed Weight QTLs in Soybean

100-seed weight is a very complicated quantitative trait of yield. The study of gene mapping for yield trait in soybean is very important for application. However, the mapping result of 100-seed weight was dispersed, the public map should be chosen which was suitable for the published results integrated, and to improve yield. In this research, an integrated map of 100-seed weight QTLs in soybean had been established with soymap2 published in 2004 as a reference map. QTLs of 100-seed weight in soybean were collected in recent 20 yr. With the software BioMercator 2.1, QTLs from their own maps were projected to the reference map. From published papers, 65 QTLs of 100-seed weight were collected and 53 QTLs were integrated, including 17 reductive effect QTLs and 36 additive effect QTLs. 12 clusters of QTLs were found in the integrated map. A method of meta-analysis was used to narrow down the confidence interval, and 6 additive QTLs and 6 reductive QTLs and their corresponding markers were obtained respectively. The minimum confidence interval (C.I.) was shrunk to 1.52 cM.These results would lay the foundation for marker-assisted selection and mapping QTL precisely, as well as QTL gene cloning in soybean.     

S100A4 (metastasin) positive mesenchymal canine mammary tumour spheroids reduce Tenascin C synthesis under DMSO exposure in vitro

In breast cancer research S100A4‐positive tumour‐associated stromal cells are assumed as primary source of Tenascin C (TNC) in the metastatic environment. Aim of the present study was to isolate and characterize S100A4/TNC positive stromal canine mammary tumour (CMT) cells. Cells grown as scaffold‐free spheroids were investigated for S100A4, TNC, and proliferative activity under 1.8% DMSO stimulation by means of Western blot and immunohistochemistry. DMSO is a commonly used drug solvent despite well‐known side effects on cells including TNC expression. DMSO did not affect proliferation, but TNC was significantly reduced under DMSO exposure for 7 and 14 days, whereby for S100A4 a reducing effect was only observed after 14 days. Without DMSO, cells stably expressed TNC and S100A4 which makes them suitable to be used in experimental approaches requiring S100A4/TNC expressing CMT stromal cells. Results show that 1.8% DMSO should not be used as solvent for experiments concerning TNC/S100A4 expression.     

S100A6 promotes proliferation and migration of human osteosarcoma cell line 143B through PI3K/Akt signaling pathway

AIM: To investigate the effect of PI3K/Akt signaling pathway on S100A6-induced proliferation and migration of human osteosarcoma cell line 143B. METHODS: Recombinant human S100A6 protein (rhS100A6) was prepared. The 143B cells were treated with rhS100A6 in the presence or absence of PI3K inhibitor (LY294002 or wortmannin) exposure. The final concentrations of rhS100A6, LY294002 and wortmannin were 30 mg/L, 10 μmol/L and 0.5 μmol/L, respectively. The expression levels of total Akt (t-Akt) and phosphorylated Akt (p-Akt) in the 143B cells were analyzed by Western blotting. The cell proliferation and migration were determined by MTT and Transwell assays. RESULTS: rhS100A6 protein was successfully prepared, and significantly increased the proliferation and migration of 143B cells (P<005). rhS100A6 up-regulated the phosphorylation of Akt in 143B cells (P<005). Compared with rhS100A6 group, the level of p-Akt in 143B cells and the proliferation and migration of the cells were decreased in combined treatment group of rhS100A6 with LY294002 or wortmannin (P<005), where the proliferation rate at different time points dropped from 10.3% to 69.7% (P<005), and the migration rate dropped from 34.9% to 47.7% (P<005). CONCLUSION: To some extent, S100A6 promotes proliferation and migration of human ostersarcoma cell line 143B through PI3K/Akt signaling pathway.     

抗菌肽CecropinB对人工感染大肠杆菌雏鸡的治疗效果研究

以1日龄来航蛋雏鸡为试验对象,研究重组抗菌肽CecropinB治疗雏鸡大肠杆菌感染的效果.120只雏鸡分6个组,每组20只,其中不同剂量抗菌肽治疗组又分为口服和肌内注射两个不同的处理,每个处理10只.致死剂量大肠杆菌肌内注射24 h后,肌内注射和口服不同浓度的重组CecropinB抗菌肽,连续治疗5 d.结果表明,重组抗菌肽CecropinB对大肠杆菌病有较好的治疗效果.其中高剂量(0.75 mL)口服组的效果最佳,治愈率达到80%,中剂量(0.5 mL)口服组与高剂量肌内注射组治愈率同为70%.中剂量肌内注射组与环丙沙星组效果一致,治愈率达到60%,低剂量抗菌肽治疗组治疗效果不如上述所有治疗组.与感染对照组相比,所有组别的雏鸡死亡率显著降低.     

1987—1991年《沈阳农业大学学报》综合分析与评价(Ⅰ)

对全国30家农业高校学报分析结果表明,有80％的学报采用计算机排版胶印,而仅有20％的学报仍采用落后的铅字排印,《沈阳农业大学学报》排印方式较落后,亟待采用计算机排版胶印;其次,该学报编辑人员偏少,比全国农业高校学报平均编辑人数4.63人少1.63人,并且专业编辑结构缺口,也需调整;再次,该学报编辑人均年发稿量、加工量和校对量比全国农业高校学报的平均值分别高5.1,15.4,20.4万字符/人·年,编辑人员超工作量工作;该学报的百页定价仅为0.93元,比全国农业高校学报的平均百页定价低O.88元,故需适当上调定价;该学报校外稿采用率基本稳定在15％～30％之间,而该校作者稿件外投率达64.7％,但该学报通过缩短稿件发表周期使本校稿件外投率呈逐年下降趋势。     

花生百果质量和百仁质量性状的QTL定位分析

为探索栽培种花生百果质量和百仁质量遗传机制,以花育28号和P76为亲本构建了包含146个家系的重组自交系（recombinant inbred line,RIL）群体,测定了3个环境下的百果质量与百仁质量表型数据,并利用单环境和多环境联合定位进行QTL的鉴定。结果表明,在不同环境下RIL群体百果质量和百仁质量均表现为超亲遗传。基于多环境QTL分析检测到5个与百果质量、10个与百仁质量相关的QTL。基于单环境QTL分析共检测到3个百果质量相关位点qHPW05.1、qHPW07.1和qHPW19.1,分布于3个连锁群上。其中qHPW07.1在三个环境下稳定表达,表型贡献率4.610%~8.840%;qHPW19.1在两个环境下稳定表达,表型贡献率9.985%~11.224%。检测到4个百仁质量相关位点qHSW05.1、qHSW07.1、qHSW19.1和qHSW20.1,分布于4个连锁群上。其中qHSW 07.1在两个环境下稳定表达,表型贡献率7.155%~10.464%;qHSW 19.1在三个环境下稳定表达,表型贡献率7.239%~13.845%。获得控制百果质量和百仁质量的QTL簇2个,分别位于LG07（Cluster I）和LG19（Cluster II）。本研究结果为后续相关基因克隆和花生产量性状改良提供了理论基础。     

Effect of seed size on protein and lipid contents,germination and imbibition in true potato seeds

Summary Seeds of an open pollinated crop of potato (Solanum tuberosum L.) TPS-2, cv. Kufri Jyoti, were graded into four sizes. There were significant differences between grades for 100-seed weight, the concentrations of total proteins and ethanol soluble proteins, and total lipids and phospholipids. Large seeds contained higher levels (% dry weight) of total proteins, ethanol soluble proteins and alkali soluble proteins than small seeds and they germinated faster and had the highest percentage of germination. Small seeds had the lowest levels of total lipids, phospholipids and water soluble proteins, the longest water saturation time and the lowest germination.     

Effect of additive and carrier type on potato sprout suppression by dust formulations of tecnazene

Summary Tecnazene formulations, with kaolin and alumina as carriers respectively and with some containing Triton X-405 (15% w/w) or, Tween 80 (15% w/w) as additive, were applied on potatoes stored at 10°C and 15–18°C respectively. Tween 80 enhanced sprouting inhibition by alumina formulation while Triton X-405 reduced that with kaolin as carrier. Formulation with kaolin as carrier produced greater sprouting inhibition than that with alumina as carrier.     

食用菌苎麻骨培养物对活性艳红X-3B染料脱色研究

[目的]研究食用菌苎麻骨培养物对活性艳红X-3B染料的脱色效果。[方法]将香菇、平菇和金针菇3种常见食用菌株接种至苎麻骨培养基上培养,研究培养物对50 mg/L活性艳红X-3B溶液的降解脱色效果。[结果]香菇培养物处理3 h对染料溶液脱色率可达92.17%,平菇培养物处理3 h对其脱色率可达72.40%。此外,香菇培养物对该染料溶液具有很好的重复处理效果,但重复处理次数越多脱色效果越差。[结论]香菇和平菇的苎麻骨培养基培养物对活性艳红X-3B溶液具有较好的脱色效果。     

花粉直感效应对桂单0810百粒重的影响

【目的】研究不同玉米品种花粉对桂单0810百粒重的影响,为挖掘该品种的产量潜力奠定基础。【方法】采用完全随机区组设计,利用8个不同玉米杂交种作为父本,对桂单0810进行人工授粉,并对不同杂交种分别进行自交及姊妹交。果穗成熟后,测定自交、姊妹交及杂交果穗的籽粒百粒重。【结果】同一品种自交果穗与姊妹交果穗的百粒重间表现出不同程度的差异,亚航639和太平洋98自交果穗籽粒百粒重比姊妹交极显著降低5.47%和5.36%（P＜0.01,下同）;琛玉969、桂单0810和迪卡008自交百粒重比姊妹交增加0.35%~2.64%,其他各品种自交百粒重比姊妹交降低0.19%~2.11%,且均未达显著水平（P＞0.05,下同）。8个玉米品种的花粉均可增加桂单0810的百粒重,其中正大808、太平洋98、先达901、琛玉969和亚航639等5个品种花粉可极显著增加桂单0810的百粒重。8个玉米品种与桂单0810的杂交组合百粒重均表现出当代（F0）杂种优势（1.31%~12.70%）,7个杂交组合表现出超亲优势。杂种优势最明显的是桂单0810×正大808组合,百粒重比母本桂单0810和父本正大808分别提高12.70%和15.68%,中亲优势达14.17%。【结论】玉米品种桂单0810易接受外来玉米品种花粉,通过花粉直感效应增加自身百粒重并具有当代杂种优势和超亲优势;其花粉直感效应因父本基因型不同而异。