Platelet function in dogs: breed differences and effect of acetylsalicylic acid administration

BACKGROUND: Clinical studies investigating platelet function in dogs have had conflicting results that may be caused by normal physiologic variation in platelet response to agonists. OBJECTIVES: The objective of this study was to investigate platelet function in clinically healthy dogs of 4 different breeds by whole-blood aggregometry and with a point-of-care platelet function analyzer (PFA-100), and to evaluate the effect of acetylsalicylic acid (ASA) administration on the results from both methods. METHODS: Forty-five clinically healthy dogs (12 Cavalier King Charles Spaniels [CKCS], 12 Cairn Terriers, 10 Boxers, and 11 Labrador Retrievers) were included in the study. Platelet function was assessed by whole-blood aggregation with ADP (1, 5, 10, and 20 microM) as agonist and by PFA-100 using collagen and epinephrine (Col + Epi) and Col + ADP as agonists. Plasma thromboxane B(2) concentration was determined by an enzyme immunoassay. To investigate the effect of ASA, 10 dogs were dosed daily (75 or 250 mg ASA orally) for 4 consecutive days. RESULTS: A higher platelet aggregation response was found in CKCS compared to the other breeds. Longer PFA-100 closure time (Col + Epi) was found in Cairn Terriers compared to Boxers. Plasma thromboxane B(2) concentration was not statistically different between groups. Administration of ASA prolonged the PFA-100 closure times, using Col + Epi (but not Col + ADP) as agonists. Furthermore, ASA resulted in a decrease in whole-blood platelet aggregation. CONCLUSIONS: Platelet function is influenced by breed, depending upon the methodology applied. However, the importance of these breed differences remains to be investigated. The PFA-100 method with Col + Epi as agonists, and ADP-induced platelet aggregation appear to be sensitive to ASA in dogs.     

玉米灌浆后期百粒重变化的品种间差异分析

采用回归异质性测验法分析了黑龙江省８个玉米杂交种抽丝后３０～６０ｄ期间百粒重的变化。结果表明,此期间早熟和晚熟杂交种百粒重变化的回归系数差异显著,因而平均灌浆速率也有显著差异。     

大果沙棘不同品种果实特性比较研究

在黑龙江绥棱和内蒙磴口试验区对俄罗斯11个大果沙棘品种和蒙古1个大果沙棘品种的果实特性进行了比 较研究,结果表明:(1)黑龙江绥棱试验点百果质量在38．33-67．59 g之间,磴口试验点为32．87-63．85 g。在供 试的品种中除卡图尼在2个试验点百果质量一致外,其余品种百果质量在磴口试验点均比绥棱试验点有不同程度 的下降。引进品种除阿尔泰新闻外,其余品种的百果质量均显著低于原产地俄罗斯,反映出百果质量随纬度下降而 下降的趋势。(2)不同品种果实横径与纵径呈紧密线性相关,即随着果实纵径的增大横径随之增大。(3)果实纵 径、横径、长宽比三个指标与百果质量均达到极显著线性相关,因此可根据果实形状指标对百果质量进行预测。 (4)供试的11个大果沙棘品种果汁Vc含量在342．6-1770．3 mg·kg-1之间,与俄罗斯原产地Vc含量(470- 3300mg·kg-1)相比均有不同程度的下降。(5)供试大果沙棘品种浆果果汁中VE含量在4-29 mg·kg-1之间。 总黄酮在36-429 mg·kg-1之间,其中槲皮素、山奈酚和异鼠李素分别为21．9-310．8 mg·kg-1、1．0-9．8 mg· kg-1和13．1-133．8 mg·kg-1,很明显总黄酮主要由槲皮素和异鼠李素组成,而且二者的含量比较接近。     

Effect of foliar and soil application of urea on leaf nutrients concentrations,yield and fruit quality of pomegranate

The influence of foliar application of 1% urea and four rates of urea (100, 200, 300 and 400 g tree^?1) as soil application (deep fertilizer placement) were studied on leaf nutrients concentrations, yield and fruit quality of ‘Malas e Torsh e Saveh’ pomegranate (Punica granatum L.) during 2010 and 2011 growing seasons. Trees that received 300 and 400 g urea as soil application showed positive significant response on fruit yield, average fruit weight, aril weight percent of fruit, 100 arils weight, fruit diameter and TSS. Foliar application of urea had also significant effects on average fruit weight, aril weight percent of fruit and 100 arils weight. Nitrogen concentration increased linearly in leaves with the increase in rate of urea-applied. According to results, deep soil application of urea under the conditions of this study was more effective on pomegranate fruit yield and quality characters than foliar application of urea.     

农业经济信息空间竞争力格局分析——以山东百强县为例

文章旨在了解县域尺度农业经济信息的空间格局以及结构,探讨影响县域农业经济发展的主导因素,分析农业经济信息在农业经济发展中的重要作用。以山东省2008年26个百强县为研究对象,重点选取了反映农业经济信息的农业机械总动力等10项指标,运用主成分分析的统计方法,建立了农业经济信息的主成分载荷矩阵,从定量的角度分析了26个百强县农业经济信息状况,基于空间分析技术以可视化的方式展示了农业经济信息竞争力的空间格局,进一步分析了百强县农业经济发展的比较优势。研究表明,2008年度山东省26个百强县农业经济发展潜力呈现3个层次的发展,第一层次4个县市,第二层次8个县市,第三层次14个县市,各个层次的县市发展具有区域特色。     

大豆品种生育期、百粒重、产量间的相关研究

通过对238份大豆区试、品系鉴定和产比试验品种的开花期、结荚鼓粒时间与百粒重关系的分析表明：安阳地区种植的大豆开花期在7月22日到8月3日之间,其中以7月26日到7月31日的较多,占供试材料的78．15％;结荚鼓粒时间在62—71d之间,以64～67d的品种最多,占供试品种的82．8％;开花期和百粒重的相关系数r=-0．317;结荚鼓粒时间与百粒重呈显著正相关,r=0．642。     

微生物液体肥料SP-100对油菜苗期生长和生理生化指标的影响

通过盆栽试验,研究不同浓度微生物液体肥料SP-100对油菜苗期生长和生理生化指标的影响。结果显示,油菜叶绿素含量、根系还原活力、硝酸还原酶活性、超氧化物歧化酶活性随SP-100稀释倍数的升高而降低,而根系生物量、植株总生物量、过氧化物酶活性、脯氨酸含量的最大值出现在500倍稀释液处理的油菜中,但与300倍稀释液处理组差异不显著。综合考虑,在实际生产中,以淋施500倍SP-100稀释液为佳。     

玉米品种穗轴重和出籽率等性状的相关及遗传关系研究

以先玉335的亲本及2个美系材料和自选系为试验材料,组配16个杂交组合,在保苗61 530株/hm2的条件下进行产量比较,同时分析子粒水分、穗轴重、出籽率等相关数据,进行简单相关和遗传分析。结果表明,杂交种穗轴重与出籽率呈极显著负相关;出籽率与双亲平均穗轴重、父本穗轴重呈极显著负相关,与双亲平均出籽率、父本出籽率呈极显著正相关;百粒重与产量呈显著正相关,与子粒水分呈显著负相关,与父本百粒重呈显著正相关;产量与父本百粒重呈极显著正相关,与双亲平均百粒重呈显著正相关。穗轴重、百粒重遗传累加效应极显著,超高亲效应明显;出籽率以遗传累加效应为主,存在超亲效应。组配新组合途径可以直接利用PH6WC及Reid系统的美系材料与自选系组配,从而获得高出籽率的品种。     

基于单片段代换系的玉米百粒重QTL分析

籽粒大小是影响玉米产量的关键因素。本研究基于59份玉米染色体单片段代换系(SSSL)纯合体,对玉米百粒重性状进行2年6个试验环境的表型鉴定,利用t测验和重叠群作图的方法对SSSL内代换片段上的百粒重效应进行了QTL分析。共检测出20个百粒重QTL,分布在玉米的8条染色体上,其中14个(70.0%)在2个以上试验环境中被重复检出,4个(20.0%)在4个以上试验环境中被重复检出,在全部6个试验环境中重复检出且基因加性效应值较大的玉米百粒重QTL是位于玉米第5染色体Bin5.05区SSR分子标记bnlg278和umc1680附近的q100kw-5-3。研究结果为玉米籽粒大小性状相关基因的进一步精细定位和克隆奠定了基础。     

基于最小二乘法的Pt100曲线拟合算法的研究

采用最小二乘法对Pt100进行拟合,推导曲线方程,并进行试验。结果表明,Pt100传感器具有精度高、重复性好等特点,适合油田洗井测温和设施农业。