全文获取类型
收费全文 | 1709篇 |
免费 | 198篇 |
国内免费 | 168篇 |
专业分类
林业 | 7篇 |
农学 | 38篇 |
基础科学 | 2篇 |
15篇 | |
综合类 | 434篇 |
农作物 | 31篇 |
水产渔业 | 465篇 |
畜牧兽医 | 1022篇 |
园艺 | 13篇 |
植物保护 | 48篇 |
出版年
2024年 | 5篇 |
2023年 | 30篇 |
2022年 | 67篇 |
2021年 | 87篇 |
2020年 | 98篇 |
2019年 | 134篇 |
2018年 | 53篇 |
2017年 | 112篇 |
2016年 | 88篇 |
2015年 | 82篇 |
2014年 | 97篇 |
2013年 | 80篇 |
2012年 | 121篇 |
2011年 | 140篇 |
2010年 | 101篇 |
2009年 | 109篇 |
2008年 | 92篇 |
2007年 | 93篇 |
2006年 | 116篇 |
2005年 | 65篇 |
2004年 | 55篇 |
2003年 | 37篇 |
2002年 | 43篇 |
2001年 | 21篇 |
2000年 | 19篇 |
1999年 | 15篇 |
1998年 | 13篇 |
1997年 | 13篇 |
1996年 | 16篇 |
1995年 | 9篇 |
1994年 | 10篇 |
1993年 | 4篇 |
1992年 | 7篇 |
1991年 | 1篇 |
1990年 | 7篇 |
1989年 | 4篇 |
1988年 | 3篇 |
1987年 | 1篇 |
1985年 | 1篇 |
1984年 | 2篇 |
1983年 | 3篇 |
1982年 | 2篇 |
1981年 | 1篇 |
1980年 | 9篇 |
1979年 | 6篇 |
1978年 | 1篇 |
1975年 | 1篇 |
1956年 | 1篇 |
排序方式: 共有2075条查询结果,搜索用时 218 毫秒
91.
Andrzej K Siwicki Zdzisaw Zak John C Fuller Jr Steven Nissen Sylwia Trapkowska Edward Gbski Agata Kowalska Krzytof Kazu & Elbieta Terech-Majewska 《Aquaculture Research》2006,37(2):127-131
Studies have shown that in both in vitro and in vivo tests, β‐hydroxy‐β‐methylbutyrate (HMB) increases the nonspecific cellular and humoral immune response and protection against diseases in animals. The present study examines the influence of HMB on nonspecific humoral defense mechanisms and protection against furunculosis in pikeperch (Sander lucioperca). β‐hydroxy‐β‐methylbutyrate was fed in a pelleted ration of 50 mg kg?1 feed day?1 for 4 weeks. Blood was drawn from 12 HMB‐fed and control‐fed pikeperch. The lysozyme and ceruloplasmin activities in the plasma, total immunoglobulin (Ig) levels, and total serum protein were analysed prior to and then after 2 and 4 weeks of HMB ingestion. After 4 weeks of HMB ingestion, a challenge test was performed by injecting the fish with live pathogenic Aeromonas salmonicida bacteria. β‐hydroxy‐β‐methylbutyrate at a dose of 50 mg kg?1 feed resulted in a statistically significant (P<0.05) increase in the lysozyme activity of the plasma, total Ig, and serum protein levels. Additionally, reduced mortality (40%) after the in vivo challenge with pathogenic A. salmonicida suggested that HMB‐activated nonspecific protection against furunculosis in pikeperch. 相似文献
92.
93.
凡纳滨对虾在微囊藻毒素(MC-LR)诱导下3种抗菌肽基因的表达量分析 总被引:1,自引:0,他引:1
蓝藻水华可产生大量藻毒素,其中以微囊藻毒素危害最大。研究表明微囊藻毒素可影响包括虾类在内的多种水生生物的生长和繁殖,亦可导致虾类免疫系统受损。本研究通过实时荧光定量PCR(qRT-PCR)技术检测了在微囊藻毒素MC-LR作用下,凡纳滨对虾(Litopenaeus vannamei)体液免疫中3种主要的抗菌肽基因(Penaeidin 3、Crustin和ALF)的表达量变化情况,发现Penaeidin 3和ALF受到MC-LR影响较大,Crustin基因表达量虽有所上调,但变化不大。同时,通过再次注射鳗弧菌(Vibrio anguillarum)对3种抗菌肽在MC-LR影响下抗菌能力的检测,发现3种抗菌肽表现出相对一致的变化,即表达量均在前期下调,4 h后逐渐上调,在MC-LR处理组中的表达量低于对照组的趋势。结果表明MC-LR可能通过直接或间接的方式影响对虾3种抗菌肽基因的表达,对免疫系统造成损伤,致使对虾抗病能力减弱;这一结果表明,长期发生铜绿微囊藻水华的虾池对虾存活率下降,可能与微囊藻毒素影响对虾抗菌肽基因的表达有关。 相似文献
94.
为研究励珂健喷剂(主要成分:杨树花提取物)对10日龄内仔猪腹泻和免疫力的影响,将18头PIC母猪随机分成3组,每组6头,分别为蒸馏水组、土霉素组和励珂健喷剂组,蒸馏水组仔猪1、3、5日龄时皮肤喷洒蒸馏水1 m L/头/d,土霉素组仔猪3、7日龄时分别肌肉注射20%长效土霉素注射液0.2 m L/头/d、0.5m L/头/d,励珂健喷剂组仔猪1、3、5日龄时皮肤喷洒励珂健喷剂1 m L/头/d。结果表明,励珂健喷剂可有效降低10日龄内仔猪的腹泻率和发病率,且励珂健喷剂组腹泻仔猪的治愈速度快于土霉素组和蒸馏水组。由此证明:励珂健喷剂对10日龄内仔猪腹泻有防治作用,并且还可以增强仔猪的免疫力。 相似文献
95.
Hong-tao Chang Xiu-yuan He Yu-Feng Liu Lu Chen Quan-hai Guo Qiu-ying Yu Jun Zhao Xin-wei Wang Xia Yang Chuan-qing Wang 《Journal of veterinary science (Suw?n-si, Korea)》2014,15(3):399-407
A recombinant replication-defective adenovirus expressing the major epitopes of porcine circovirus-2 (PCV-2) capsid protein (rAd/Cap/518) was previously constructed and shown to induce mucosal immunity in mice following intranasal delivery. In the present study, immune responses induced by intranasal immunization with a combination of rAd/Cap/518 and cytosine-phosphate-guanosine oligodeoxynucleotides (CpG ODN) were evaluated in mice. The levels of PCV-2-specific IgG in serum and IgA in saliva, lung, and intestinal fluids were significantly higher in the group immunized with rAd/Cap/518 and CpG ODN than animals immunized with rAd/Cap/518 alone. The frequencies of IL-2-secreting CD4+ T cells and IFN-γ-producing CD8+ T cells were significantly higher in the combined immunization group than mice immunized with rAd/Cap/518 alone. The frequencies of CD3+, CD3+CD4+CD8-, and CD3+CD4-CD8+ T cells in the combined immunization group were similar to that treated with CpG ODN alone, but significantly higher than mice that did not receive CpG ODN. PCV-2 load after challenge in the combined immunization group was significantly lower than that in the phosphate-buffered saline placebo group and approximately 7-fold lower in the group treated with CpG ODN alone. These results indicate that rAd/Cap/518 combined with CpG ODN can enhance systemic and local mucosal immunity in mice, and represent a promising synergetic mucosal vaccine against PCV-2. 相似文献
96.
97.
B R LaFrentz S E LaPatra G R Jones J L Congleton B Sun K D Cain 《Journal of fish diseases》2002,25(12):703-713
Serum and mucosal antibody responses of juvenile rainbow trout, Oncorhynchus mykiss, were characterized by enzyme‐linked immunosorbent assay (ELISA) following immunization with various preparations of formalin‐killed Flavobacterium psychrophilum cells. The protective nature of these preparations was then determined by immunizing rainbow trout fry and challenging with the bacterium. Juvenile rainbow trout immunized intraperitoneally (i.p.) with formalin‐killed F. psychrophilum emulsified with Freund's complete adjuvant (FCA), and i.p. with formalin‐killed F. psychrophilum either with or without culture supernatant generated significant serum antibody responses by 6 and 9 weeks, respectively. Significant mucosal antibody responses were detected by 9 weeks only in fish immunized i.p. with killed F. psychrophilum/FCA. Following immunization and bacterial challenge of rainbow trout fry, protective immunity was conferred in F. psychrophilum/FCA and saline/FCA groups with relative per cent survival values of up to 83 and 51, respectively. Significant protection was not observed in treatment groups immunized by immersion or i.p. without adjuvant at the challenge doses tested. Results suggest that stimulation of non‐specific immune factors enhances the ability of fish to mount a protective immune response, but specific antibody appears necessary to provide near complete protection. In this study, an ELISA was developed to monitor anti‐F. psychrophilum antibody production in trout. The relationship of such responses to protective immunity suggests that future vaccination strategies against coldwater disease may require stimulation of both the innate and adaptive arms of the immune response. 相似文献
98.
A3α肽聚糖对凡纳对虾幼体生长及抗病毒感染力的影响 总被引:6,自引:1,他引:6
用含不同量的A3α肽聚糖(PG)(0.005%、0.01%、0.1%)的幼体饵料投喂凡纳对虾幼体,另在育苗水体中添加不同水平(0.005mg·mL-1、0.01mg·mL-1、0.05mg·mL-1)PG浸浴并以投喂不添加PG的幼体饵料的实验组作为对照,另设投喂活饵的实验组作参考,分别考察Z1-M1期、M1-PL1期、Z1-PL1期幼体的成活率,M1期、PL1期幼体体长,PL1期幼体体重,另外,PL1期仔虾经7d饲养后,检测体内酚氧化酶(PO)活性,并用白斑综合症病毒(WSSV)料投喂感染。结果显示:与投喂不添加PG幼体饵料组相比,在Z1-M1期,活饵组、0.01%PG添加量的幼体饵料组、0.005mg·mL-1PG的浸浴组幼体的成活率有极显著(P<0.01)提高,而在Z1-PL1、M1-PL1期,各实验组除0.05mg·mL-1浸浴组外均有极显著地(P<0.01)提高;各实验组除0.01mg·mL-1、0.05mg·mL-1PG浸浴组外,M1期幼体体长、PL1期幼体体重也有显著(P<0.01)增长,且各实验组PL1期幼体都有极显著(P<0.01)增长;各实验组仔虾PO活力均有极显著(P<0.01)提高;各实验组除0.1%PG添加量的幼体饵料组外,感染WSSV后仔虾的存活率有不同程度地提高。 相似文献
99.
100.
饲料Vc对大菱鲆非特异性免疫力的影响 总被引:1,自引:1,他引:1
X用维生素C(Vc)含量为0~2500mg/kg的配合饲料连续投喂大菱鲆70d后,测定大菱鲆血液白细胞的总数、吞噬活性、血清溶菌酶活性和总补体活性。结果表明,饲料中Vc含量增加到250mg/kg,白细胞总数明显增加,进一步提高Vc含量,白细胞总数没有显著变化;血清溶菌酶活性在Vc含量为250mg/kg时最高,其他各组无显著性差异;添加Vc的各试验组的总补体活性明显地高于对照组,但是添加Vc的各组之间的总补体活性没有显著性差别;Vc含量对白细胞的吞噬活性没有影响。 相似文献