四川小麦品种高、低分子量麦谷蛋白基因和1B/1R易位的分子标记鉴定

为给四川小麦品质育种提供参考信息,利用7个HMW-GS、17个LMW-GS和1个1B/1R易位的特异分子标记,对105份2000年后育成的四川小麦品种进行上述基因检测。结果表明:(1)针对HMWGS,在Glu-A1位点,含Ax2*的品种有2份,频率为1.9%;在Glu-B1位点,含Bx7、Bx20、Bx17、By8和By9的品种分别有73、26、4、45和30份,频率分别为69.5%、24.8%、3.8%、42.9%和28.6%,未检测到含Bx7OE的品种;在Glu-D1位点,含Dx5的品种有65份,频率为61.9%。(2)针对LMW-GS,在Glu-A3位点,含Glu-A3a、Glu-A3b、Glu-A3c、Glu-A3d和Glu-A3f的品种分别有2、2、63、29和9份,频率分别为1.9%、1.9%、60.0%、27.6%和8.6%,未检测到含Glu-A3e和Glu-A3g的品种;在Glu-B3位点,含Glu-B3b、Glu-B3d、Glu-B3f、GluB3g和Glu-B3i的品种分别有18、10、1、75和1份,频率分别为17.1%、9.5%、1.0%、71.4%和1.0%,未检测到含Glu-B3a、Glu-B3c、Glu-B3e和Glu-B3h的品种。(3)含1B/1R易位的品种有36份,频率为34.3%。(4)组合6种和5种以上优质基因的品种分别有2份(频率为3.8%)和15份(频率为14.3%)。可利用这些品种作为亲本,在四川小麦品质育种中逐步导入优质基因Ax2*、Bx7、By8、Dx5、Glu-A3d、Glu-B3d、Glu-A3b和Glu-B3b,并淘汰1B/1R易位,优化四川小麦面筋优质基因组成。     

Deferoxamine Compensates for Decreases in B Cell Counts and Reduces Mortality in Enterovirus 71-Infected Mice

Enterovirus 71 is one of the major causative agents of hand, foot and mouth disease in children under six years of age. No vaccine or antiviral therapy is currently available. In this work, we found that the number of B cells was reduced in enterovirus 71-infected mice. Deferoxamine, a marine microbial natural product, compensated for the decreased levels of B cells caused by enterovirus 71 infection. The neutralizing antibody titer was also improved after deferoxamine treatment. Furthermore, deferoxamine relieved symptoms and reduced mortality and muscle damage caused by enterovirus 71 infection. This work suggested that deferoxamine has the potential for further development as a B cell-immunomodulator against enterovirus 71.     

Modification of Marine Natural Product Ningalin B and SAR Study Lead to Potent P-Glycoprotein Inhibitors

In this study, new marine ningalin B analogues containing a piperazine or a benzoloxy group at ring C have been synthesized and evaluated on their P-gp modulating activity in human breast cancer and leukemia cell lines. Their structure-activity relationship was preliminarily studied. Compounds 19 and 20 are potent P-gp inhibitors. These two synthetic analogues of permethyl ningalin B may be potentially used as effective modulators of P-gp-mediated drug resistance in cancer cells.     

竹炭基生物炭对茶叶品质和土壤微生物群落结构的影响

为探讨竹炭对茶叶品质、土壤微生物群落结构及土壤酶的影响,本研究以多年种植的茶园土为对象,茶叶为供试作物,设置5个处理:不施肥对照处理(CK₁);有机肥7 500 kg·hm^-2对照处理(CK₂);有机肥7 500 kg·hm^-2+竹炭1 125 kg·hm^-2处理(T₁);有机肥7 500 kg·hm^-2+竹炭2 250 kg·hm^-2处理(T₂);有机肥7 500 kg·hm^-2+竹炭3 375 kg·hm^-2处理(T₃),探究茶园多年种植条件下土壤微生物和胞外酶对施入竹炭的响应机制。结果表明,竹炭的施入对土壤微生物总生物量(T)、细菌生物量(B)、真菌生物量(F)、茶叶产量(Y)、Mg含量、过氧化氢酶(PER)活性、酸性磷酸酶(ACP) 活性、β-葡萄糖苷酶(BG)活性和蔗糖酶(SU)活性具有促进作用,各处理表现为T₂>T₁>T₃> CK₂>CK₁;对放线菌生物量(A)、真菌/细菌(F/B)比值、百芽重(BW)、发芽密度(BD)、水浸出物(WE)、咖啡碱(Caf)、茶多酚(Po)、氨基酸(Am)、儿茶素(Cat)和Vc含量也具有促进作用,各处理表现为T₂>T₃>T₁>CK₂>CK₁。竹炭施入通过改变F/B比值和酚氨比,从而改善了病土食物网结构、土壤生态系统食物网营养结构以及茶叶的品质。在外界种植环境相同的条件下,随着竹炭添加量的增加,T、B、F、A、F/B、BG活性、PER活性、ACP活性、SU活性呈现先增加后减少的趋势,酚氨比、G^-/G⁺呈现下降趋势,压力指数先减少而后升高。竹炭添加量为2 250 kg·hm^-2(T₂)时更为有效。本研究结果可为深入解析茶叶品质、茶园土壤质量演变特征与保护提供科学依据。     

UV-B胁迫下芦荟蒽醌类物质对大豆生长发育保护作用研究(英文)

在增强紫外线辐射条件下,研究喷施芦荟蒽醌类物质对大豆叶形态结构和生理生化指标的影响,旨在探究增强UV-B辐射条件下芦荟蒽醌物质对植物的保护作用。研究结果表明,在增强UV-B辐射条件下,大豆株高降低、叶面积明显减小、光合色素含量下降、丙二醛含量升高;喷施芦荟蒽醌物质能有效缓解叶绿素含量、生物量降低,减少丙二醛产生;在增强UV-B辐射条件下,叶细胞表面蜡状物质增加明显,气孔明显下陷;而喷施芦荟蒽醌物质后,表面蜡状物质仅稍有增加,气孔下陷状况有所缓解。     

Alternate Two Different Modes of Actions of Green Chemicals to Combat Batrachedra amydraula Meyrick and Cadra cautella Walk on Date Palm Fruit,Egypt

Alternate two different modes of actions of green chemicals： spinosad ＆ methoxyfenozide to combat the lesser date moth, Batrachedra amydraula and the Almond moth, Cadra cautella Walk on dates in Dakhla Oasis, New Valley, Egypt were done in 2010 and 201 I. Based on infestation of the early insect pest B. amydraula, the three-rotation program with basic 1st spray of spinosad 24 SC at 20 mL/100 L showed insignificant difference. Based on minimum effective rotation doses, alternating spinosad 24 SC at 20 mL/100 L, then methoxyfenozide 24 SC at 15 mL/100 L with two-week interval showed zero infestation and 1.8% at the last sample of 2010 and 2011, respectively. Based on infestation of the late insect pest, Cadra spp., tlhe same previous trend of the three-rotation results. Based on minimum effective rotation doses, alternating spinosad 24 SC at 20 mL/100 L, then methoxyfenozid 24 SC at 15 mL/100 L with three-week interval indicated less than 1% and 1% infestation during harvest for 2010 and 201 l, respectively. The last reflected important value when storing the fruit. Both products proved to be working under dry hot high temperatures.     

安徽省知母药材质量研究

[目的]研究安徽产知母药材中知母皂苷BⅡ和芒果苷的含量.[方法]知母皂苷BⅡ采用Diamonsil C18柱（250 mm ×4.6 mm,5μm）、乙腈-水等度洗脱（25∶75）,流速1.0 ml/min、ELSD检测器漂移管温度60℃、载气流速3.0 ml/min、柱温为室温（25℃）;芒果苷采用Diamonsil C18柱（150 mm×4.6 mm,5μm）、乙腈-0.2％冰醋酸水溶液（15∶85）等度洗脱,流速1.0 ml/min.[结果]知母皂苷BⅡ在1.036～10.360 μg范围内（r=0.999 6）进样量的对数值与峰面积的对数值呈良好的线性关系,芒果苷在0.05 ～1.00 μg范围内（r=0.9994）进样量与峰面积呈良好的线性;知母皂苷BⅡ平均回收率为99.7％,RSD=1.19％;芒果苷平均回收率为100.3％,RSD=1.42％.[结论]安徽省知母药材中知母皂苷BⅡ与芒果苷含量均符合2010版药典含量标准.     

HBV衣壳蛋白装配抑制剂的研究进展

乙型肝炎病毒(HBV)是一种常见的嗜肝性病原体,全球约有4亿人感染,带来严重的社会问题和经济负担。虽然目前已有预防和治疗HBV的药物,但由于耐药性等问题不能彻底清除病毒感染,新药开发依然迫在眉睫。介绍了HBV的病毒结构、复制和感染过程,对现有治疗药物的优缺点进行了总结。对以衣壳蛋白装配为靶点的新药研究进展进行综述,旨在为今后开发抗HBV的新药提供参考。     

生鲜农产品网络销售系统设计与实现

介绍利用PHP＋ Oracle数据库开发技术,基于B/S结构,采用MVC框架开发模式,以上海地区生鲜农产品网络销售实际需求应用出发,设计和实现B2B2C模式的生鲜农产品网络销售系统.分别从系统需求分析、系统功能模块设计、数据库设计和系统实现相关技术等几个方面进行详尽阐述.     

基于 FY3B／MERSI水稻长势监测及其与 AQUA／MODIS数据对比分析

为利用中分辨率光谱成像仪FY3B/MERSI数据进行水稻种植面积信息提取及长势监测,首先对FY3B/MERSI与AQUA/MODIS单通道反射率进行对比分析;其次,通过目视解译和遥感监督分类方法提取了环鄱阳湖区双季早稻的空间分布;最后,基于面积识别结果,利用NDVI对环鄱阳湖区双季早稻长势进行监测。结果表明：（1） FY3B/MERSI与AQUA/MODIS水稻种植区红光和近红外通道反射率具有极显著相关性,且MERSI红光波段及近红外波段反射率都略小于对应MODIS反射率;（2）水稻的提取精度总体达到了92％,可以满足其生育期长势遥感监测的需求;（3） FY3B/MERSI和AQUA/MODIS计算的水稻区NDVI存在显著正相关,且二者测得的水稻长势均与实际苗情数据基本吻合,因此,由FY3B/MERSI遥感影像直接得到的水稻苗情分类基本可以满足业务化需要。