RT-PCR技术诊断猪瘟的应用研究

应用反转录—聚合酶链反应(RT-PCR)对猪瘟进行诊断应用研究。应用RT-PCR况对来自广西不同地区的135份疑似猪瘟病料进行检测，以份诊断为阳性，阳性率62．2％。从百色、柳州地区等采集的健康猪扁桃体和淋巴结共276份，经RT-PCR检测，37份为阳性，阳性率为13．4％。其中健康猪扁桃体带毒较高，246份扁桃体中有35份阳性，占14．2％。采自柳州健康猪的26份淋巴结材料全为阴性，只有邕宁县的1份健猪淋巴结阳性。结果表明，RT-PCR技术可应用于猪瘟的临床诊断。     

中国结缕草属(Zoysia spp.)植物抗寒性评价

在广泛收集结缕草属(ZoysiaWild.)种质资源基础上,按照形态类型和地理分布,选取40份种质,采用电导法(EL)对其抗寒性进行初步评价。以半致死温度(LT50)为评价指标,在参试材料中除大穗结缕草和来自台湾岛海边沙地的沟叶结缕草外,其它种质都较天堂-419(对照)抗寒;在结缕草属内,抗寒性具有明显的种间差异,耐寒性依次为日本结缕草>中华结缕草>沟叶结缕草>细叶结缕草>长花中华结缕草>大穗结缕草,其中细叶类型的沟叶结缕草和细叶结缕草抗寒性变异较大;日本结缕草和中华结缕草抗寒性变异与地理分布之间没有显著关系,但分布在海边的种类和种质抗寒性明显较低;结缕草属及其中华结缕草的抗寒性与叶长、叶宽以及叶背面被毛之间均存在显著的负相关关系,但这种关系不体现在日本结缕草上。实验结果还表明,EL方法是评价结缕草属抗寒性较为可靠的方法之一。     

An evaluation of the pesera soil erosion model and its application to a case study in Zakynthos, Greece

Abstract. The Pan‐European Soil Erosion Risk Assessment (pesera ) model was evaluated using existing soil erosion data collected under various types of climate, vegetation, landscape and soil conditions. The data used represent a variety of typical Mediterranean land uses such as winter wheat, vines, olives and bare, stony land prevailing in hilly areas. Using this data, the model was calibrated for sediment transport by overland flow and results compared to measured soil erosion values from runoff plots and a watershed on a monthly basis. The performance of the model was assessed statistically, showing that it can be satisfactorily used for predicting soil erosion rates under the conditions included in the study. The overall model estimate including all the available experimental data was 0.69 t ha^?1 yr^?1 with a maximum error of 1.49 t ha^?1 yr^?1. After validation, the model was applied to a small watershed (60 ha) of great ecological importance for the sea turtle Caretta caretta. For this purpose, soil and vegetation maps were compiled from all the necessary data for applying the model. The model was run for three years using daily data from an existing nearby meteorological station. The predicted and measured soil erosion rates for a 7‐month period were 0.31 t and 0.18 t, respectively. Application of the model to each mapping unit showed the over‐riding importance of land use for sediment generation under the given climatic conditions. Bare land, occupying 5.5% of the watershed area, generated up to 69% of the total sediments estimated for the watershed. It is concluded that the pesera model can be used as a regional diagnostic tool under a range of soil, topographic and climatic conditions for identifying the best land use type and vegetation cover to protect hilly areas from soil erosion. The calculated overall root mean square error for the model is 0.06 t ha^?1 yr^?1, compared to a soil erosion rate of 0.04 t ha^?1 yr^?1, which can be tolerated for protecting the area for the sea turtle.     

创造性思维教学的探索与实践

随着素质教育的实施，培养学生的创造精神与实践能力，已成为世界各国教育的趋势。为此．就高等院校如何推行创造性思维教学、培养学生的创造性思维能力进行了探讨。     

甲醛捕捉剂在胶合板生产中的应用

普通UF树脂胶中添加甲醛捕捉剂H300或H800后,其固化时间明显延长.采用H300或H800改性的UF醛树脂胶黏剂生产胶合板,在保证胶合强度的前提下,板材的甲醛释放量达到国标E2或E1级要求.     

Multi-sectoral Participatory Urban Forestry Development in Philippines Metropolitan Manila

The Luntiang Kamaynilaan Project (Metro ManilaGreening Project) launched in 1988 planted trees inmajor thoroughfares, center islands, school premisesand other idle public lands, was basically a governmenteffort. When the funds ran out, the project also fizzledout. In both projects however, it is evident that the keyfactor is peoples participation.Gaining lessons from the past two projects, thegovernment established a collaborative effort ofgovernment and non-government organizations. TheCl…     

矮壮素对高羊茅生长特性影响的研究

试验采用0.8,1.0,1.2 mg/g 3种浓度的矮壮素(CCC)对草坪草高羊茅Festuca arundinacea进行处理,结果表明:3种浓度的矮壮素对高羊茅生长均产生不同程度的影响,其中 0.8 mg/g矮壮素处理使高羊茅株高、修剪量明显降低.综合考虑药效及成本,建议在实际生产中应用0.8 mg/g的矮壮素浓度.     

虚拟现实与果树修剪

虚拟现实是近年来发展迅速、引人关注的研究热点。本文提出了虚拟现实在农业领域——果树修剪技术推广中应用的可能性。简述了虚拟现实的基本概念和应用现状，并就“虚拟果树修剪系统”提出了初步设想和基本思路。     

GIS在生态土地分类中的作用

This paper depicted the physiographic landscape features and natural vegetation situation of study area (the eastern Jilin Province), and expatiates the definition, basic characters and its development of Ecological Land Classification (ELC). Based on the combination of relief map, satellite photography for study area and vegetation inventory data of 480 sample sites, a 5-class and a 15-class ecological land type map was concluded according to 4 important factors including slope, aspect, vegetation and elevation. Ecological Classification System (ECS) is a method to identify, characterize, and map ecosystems. The Ecological Land Type (ELT) was examined and applied initially in eastern Jilin Province. Foundation item: This paper was supported by Chinese Academy of Sciences “100 people” project and the Open Research Station of Changbai Mountain Forest Ecosystem. Biography: XIAO Bao-ying (1974-), female, postgraduate in Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, P. R. China Responsible editor: Zhu Hong     

含蜡原油管道蜡沉积研究进展

蜡沉积会减少管道的有效内径，增大输送压力，降低管道的输送能力，增加清管频率，甚至造成蜡堵事故。介绍了国内外有关析蜡的热力学模型、蜡沉积机理及蜡沉积的动力学模型的研究进展状况，提出了一些研究建议。