鸡新城疫病毒F基因和鸡IL—2重组DNA疫苗的构建

利用已克隆到的新城疫D26株F基因和鸡IL－2基因，经过载体改建，将他们共同克隆于真核表达质粒pCDNA3上，经酶切分析、PCR鉴定证实成功构建了共表达鸡新城疫病毒F基因和鸡IL－2的重组质粒，为探讨禽类重组基因疫苗的构建及鸡IL－2在基因疫苗中的作用奠定了基础。     

中国野生种毛葡萄光合特性的研究

毛葡萄（ＶｉｔｉｓｑｕｉｎｑｕａｎｇｕｌａｒｉｓＲｅｈｄ）的净光合速率日变化值明显高于山葡萄、宝石、白玉霓，且无栽培品种光合作用的“午休”现象。毛葡萄的净光合速率与其它野生种葡萄和栽培品种比较也表现出较高水平。在进行ＣＯ２饱和点和补偿点测定时，毛葡萄也较栽培品种白玉霓有较强的光合作用，因此，毛葡萄是葡萄高光合效率育种的优良野生种质资源。     

“两田制”与农业双层经营

虽然双层经营体制已经得到确立,但至今其经营客体或对象在实践中仍然是不清楚的,而且就统分结合两方面来看,统的职能也严重不足,致使家庭经营的弊端依然存在,双层经营的功能难以充分发挥.为此.笔者提出,“两田制”是完善双层经营体制的有效形式。它有利于实现农业的适度规模经营,可大幅度地提高农业劳动生产率和农产品商品率,还形成了农村劳动力合理流动的机制,有利于农村经济的全面发展和农业机械化的推广。所以,笔者主张,“两田制”应谈有计划地加以推广.     

Sensitivity of four Yeasts to Fungicides and CO2 Concentrations and Their Antagonistic Ability in Combination with Fungicide to pathogenic Fungi in vitro

The yeasts Cryptococcus laurentii,Trichosporon pullulans,Rhodotorula glutinis and Trichosporon sp.were used to investigate their sensitivity to four fungicides and different concentrations of CO2,as well as their antagonistic ability to Penicilliumexpansum and Alternaria alternata in vitro when applied with fungicide.There were significant differences in sensitivity to the fungicides among the different yeasts(P = 0.05).R.glutinis was more sensitive to Deccocil,Iprodione and Stroby as compared to other yeasts.Combination antagonistic yeasts with fungicide could more significantly enhance biocontrol ability of the yeasts against the pathogenic fungi in vitro(P = 0.05).C.laurentii was the most effective antagonist among the four yeasts and could completely control spore germination of P.expansum and A.alternata when combined with Stroby at the concentration of 100 μL L-1.The yeasts,except R.glutinis,could grow well on nutrient yeast dextrose agar (NYDA) after 8 d incubation even at 20% CO2 concentration at 25℃.Particularly Trichosporon sp.showeda better adaptability to low temperature as compared to other antagonists.     

一条不对称合成S(+)-2-氨基-4-磷酸基丁酸的新路线

利用4-二乙氧基膦酰基-2-酮丁酸乙酯与 L-赤式-(+)-1,乙-二苯基-2-氨基乙醇的缩合,将产物还原、氢解及水解得光活性产物,ee 值67%。     

白细胞介素和干扰素γ对绵羊体外受精胚胎体外发育影响的研究

利用SOFM+0.8%BSA+1mM谷氨酰胺作为绵羊胚胎的培养液,分别添加LIF、IL-1β、IL-2、IL-6及IFN-γ,以研究LIF、IL-1β、IL-2、IL-6及IFN-γ对绵羊早期胚胎发育的影响。结果说明,LIF促进2～8细胞胚胎发育至桑椹胚,同时也能够促进桑椹胚向扩大囊胚和孵化囊胚发育。但IL-1β、IL-2、IL-6及IFN-γ对绵羊早期胚胎的发育无显著影响。     

猪肺炎支原体黏附因子基因R1R2区的克隆及表达

根据GenBank登录的猪肺炎支原体232株P97基因和J株黏附因子基因设计了1对引物，以我国猪肺炎支原体Z株(强毒株)基因组DNA为模板，通过PCR方法扩增了该株黏附因子基因的部分序列。经序列分析后，重新设计了1对带有EcoRI和HindⅢ酶切位点的引物，并经引物的定点突变，PCR扩增了Z株黏附因子的R1R2区。扩增产物经双酶切后克隆到表达载体pET-32(a) 中。该重组质粒经酶切鉴定后，将其具有正确阅读框架的重组质粒转化到大肠杆菌BL21(DE3)感受态细胞，37℃下经IPTG诱导表达，得到相对分子质量约29000的融合蛋白，表达量约为11％。     

超临界CO_2流体处理工艺对木材渗透性的影响

以苯—乙醇为夹带剂,研究超临界CO2流体不同处理压力、温度、时间对福建产杉木、马尾松、檫木木材渗透性的影响。结果表明,在35 MPa内,处理压力的增大有利于3种木材渗透性的提高;杉木在处理温度为40℃时,渗透性的变化率达到最大,为493%,马尾松和檫木在处理温度为45℃时,渗透性的变化率达到最大,分别为479%和349%;较佳的处理时间为30 min。     

Estimating Combining Ability of 4x-2x Crosses in Cultivated Potatoes

Progenies derived from crosses between Solarium tuberosum and 2n pollen-Producing diploid hybrids, exhibit obvious hybrid vigor. The 2n pollen-producing clone can act as a bridge in crossing S. tuberosum and S. andigena with S. phureja. Populations from 4x-2x crosses show more unifomity and less segregation compared with that of 4x-4x crosses. The parent-offspring correlation for the traits, starch content and tuber number, is significant at 0.01 level. The regrssion equations are Y (mp-F1)=1.0 1.2x and Y (mp - F1) = 5.3 0.8x, respectively. The 2n pollen-producing clones play an impotant role in increasing tuber stach content. Estimates of the combining ability for the main yield components indicate that additive effect prodominatcs for such trais as plot yield, tuber weight per plant and starch content, whereas both additive and non-additive effects lay equal stress on mean tuber weight and non-additive effect is important for tuber number. In general, non-additive effect appears to be important in