ZB25/ZB45型包装机条盒反包检测装置的研发

为了避免反包条烟直接流入消费者市场,消除条烟反包质量问题,利用具有光洁度（粗糙度）识别的检测器对ZB25型软盒硬条包装机和ZB45型硬盒硬条包装机原有的检测系统进行完善改造。介绍了光洁度（粗糙度）检测器的工作原理以及条盒反包检测的电路设计。增加条盒反包检测后,在生产过程中反包条烟能自动识别并被剔除,消除了条烟反包质量问题,取得了很好的效果。     

Advance on Foot-and-mouth Disease Virus

Foot-and-mouth disease (FMD) is an acute,febrile and highly contagious animal disease caused by foot-and-mouth disease virus (FMDV),and has been recognized as the most important constraint to international trade in animals and animal products.An outstanding feature for FMDV infection is that the FMDV infected animals may remain as a carrier state,some of the animals exposed to FMDV may have a long term asymptomatic infection.This article will review the advance of FMDV in the following aspects,epidemiology,etiology and pathogenesis.     

Pathogen Analysis of Bovine Respiratory Disease Complex

To investigate the pathogen of bovine respiratory disease complex (BRDC) of a dairy farm in Guangxi, a strain of Mycoplasma and a strain of gram-negative pathogenic bacterium were isolated and identified by the means of field surveys, clinic observation, pathological examination, isolation studies and so on.Treatments were taken according to drug sensitivity test results.The Mycoplasma strain, growing on PPLO medium, formed typical "fried egg" colonies.A 448 bp of oppF fragment was amplified by PCR from the strain and had 98.4% nucleotide identity with Mycoplasma bovis reference isolate PG5 of USA.The biochemical features of the gram-negative bacterial isolate were same with Serratia marcescens.The PCR amplified 16S rDNA of the gram-negative pathogenic bacterium strain was 1 400 bp.It shared 99.0% nucleotide identity with other Serratia marcescens reference strains obtained from GenBank.Animal experiment showed that the gram-negative pathogenic bacterium isolate could cause the mice to die.The drug sensitivity tests showed all isolates were sensitive to spectinomycin, azithromycin, amikacin, gentamicin and neomycin.It was effective to treat with dexamethasone and spectinomycin.Pathogen analysis and drug treatment showed that the BRDC was caused by Mycoplasma bovis and Serratia marcescens.     

Construction and Identification of PRRSV ORF5 Gene Prokaryotic Expression Vector

The present study was designed to construct recombinant plasmids,which could express porcine reproductive and respiratory syndrome virus (PRRSV) ORF5 gene.RNA was extracted from spleen and lung samples of the suspected pigs which were infected with PRRSV.According to PRRSV ORF5 gene,a pair of primers was designed for RT-PCR amplification.The ORF5 target gene was cloned into pMD19-T vector and then the recombinant pMD19-ORF5 was achieved.According to the sequencing results and the characteristics of expression vectors,a pair of primers with NcoⅠand XbaⅠenzyme cleavage sites was designed.Target fragment dORF5 was amplified and then connected to pProEXHTb and pNZ8149 vectors,respectively.And recombinant HTb-dORF5/DE3 and pNZ8149-dORF5/NZ3900 was induced by IPTG and Nisin,respectively,and analyzed by SDS-PAGE and Western blotting.Recombinant HTb-dORF5/DE3 induced by 1.5 mmol/L IPTG was expressed in the highest quantity.There were specific band at about 22 ku with reactionogenicity when it was tested by SDS-PAGE and Western blotting.Recombinant pNZ8149-dORF5/NZ3900 induced by 20 ng/mL Nisin was expressed in the highest quantity.There were specific band at about 19 ku with reactionogenicity when it was tested by SDS-PAGE and Western blotting.The IFA result showed specific green fluorescence.This study successfully constructed recombinant plasmids HTb-dORF5 and pNZ8149-dORF5 and expressed,the result laid a solid foundation for further development of PRRS vaccines.     

Daily variation in body mass and thermoregulation in male Hwamei(Garrulax canorus) at different seasons

Background: Acclimatization to winter conditions is an essential prerequisite for survival of small passerines of the northern temperate zone. In the present study, we measured diurnal variations in body mass, body temperature and basal metabolic rate(BMR) for seasonally acclimatized Hwameis(Garrulax canorus).Methods: Body mass was determined with a Sartorius balance. Metabolic rates of Hwameis were measured with an open-circuit respirometry system.Results: Body masses varied with time of day and were higher in daytime for Hwameis in both summer and winter, and body masses in winter were higher compared to that in summer. Body temperatures of Hwameis were higher in daytime, and the summer acclimatized birds had significantly higher body temperatures compared to the winter acclimatized birds. BMRs of Hwameis were significantly higher during the daytime compared to the nighttime of the daily cycle in both summer and winter, and Hwameis in winter had significantly higher BMRs than that in summer.Conclusions: This result showed that Hwameis rely mostly on metabolic capacity to maintain their body temperature in cold weathers, and Hwameis exhibited daily and seasonal flexibility in morphology and physiology which is important under changing environmental conditions.     

黑荆树树皮单宁组分研究——Ⅲ.原花色素的分离鉴定及其~(13)C NMR特征(英文)

从我国江西四年生黑荆树(Acacia mearnsii)树皮单宁中分离鉴定了三个二聚原花色素:刺槐亭醇-(4α→8)-儿茶素、菲瑟亭醇-(4β→8)-儿茶素和刺槐亭醇-(4β→8)-儿茶素。其中刺槐亭醇-(4β→8)-儿茶素为首次从黑荆树树皮中以天然形式分离得到,为一新天然化合物。文中首次对黑剂树树皮二聚原花色素的~(13)CNMR进行了报道,并就其特征和确定二聚原花色素的相对和绝对构型进行了讨论。     

白马鸡人工驯养初步研究

通过人工饲养的方式探索出白马鸡驯养的经验,即:在野生白马鸡适宜生境下建立饲养场,从育雏阶段开始进行人工投食饲养和本地土鸡带领相结合的驯养方式。目前已经完成从子一代到子二代的完整驯养、饲养流程,取得了云南省林业厅的驯养繁殖许可证。对育雏、亚成体、成体阶段的喂食量、存活情况等作了分析,认为接近原始生境的环境及本地带雏母鸡是成功的重要条件。     

α-萘乙酸对林芝凤仙花扦插的作用研究

林芝凤仙花是中国西藏特有植物,可作为地区特色观赏植物进行开发.为寻找一种高效的人工培育技术,本试验在保护地条件下,以一年生林芝凤仙花为材料,研究了不同浓度NAA(α-萘乙酸)对林芝凤仙花扦插繁殖效果的影响.试验结果表明:(1)林芝凤仙花是扦插易生根类型植物,能在节部和节间生根,但以节部生根为主.(2)林芝凤仙花插穗的取...     

DPF再生对柴油机性能的影响

非道路国四柴油机主要有两种控制技术路线:DOC+DPF+SCR与EGR+DOC+DPF,由于国四排放标准中PN限值,DPF技术运用成为首选。从柴油机排气温度对DPF再生的影响、柴油机排气流量对DPF再生的影响、柴油机尾气中NO_(2)含量对DPF再生的影响、柴油机尾气中O_(2)含量对DPF再生的影响和不同微粒对DPF再生特性的影响方面进行分析,阐述DPF再生对国四柴油机性能使用的重要影响,为非道路国四柴油机DPF结构优化和国四拖拉机使用提供参考。