用绵羊小肠液冻干粉测定饲料过瘤胃淀粉小肠消化率方法的研究

本试验旨在以玉米为样本筛选用绵羊小肠液冻干粉测定精饲料瘤胃非降解残渣淀粉小肠消化率的最佳培养条件。分别研究了小肠液冻干粉用量(0.2、0.3、0.4、0.5和0.6g)对16h玉米瘤胃非降解残渣干物质和淀粉小肠消化率的影响;离体培养时间(4、8、12、16、20和24h)对16h玉米瘤胃非降解残渣干物质和淀粉小肠消化率的影响。结果表明,在本试验条件下,绵羊小肠液冻干粉测定玉米瘤胃非降解残渣淀粉小肠消化率的最佳用量为0.45g小肠液冻干粉/0.56g玉米瘤胃非降解残渣,最佳培养时间为12h。利用绵羊小肠液冻干粉测定常用饲料过瘤胃残渣淀粉小肠消化率的方法是可行的。     

Comparison of S-RNase,RNase T1, T2, and A effects on growth inhibition and RNA degradation of in vitro-cultured pear pollen

To obtain the basic information on fruit set regulation, effects of several RNases including S-RNase on pollen tube growth and RNA degradation in the tube were studied in the pear. Purified S-RNase from the Japanese pear ‘Kosui’ (S₄S₅) predominantly inhibited the growth of ‘Kosui’ pollen tubes (self) in vitro at 0.28 unit μL⁻¹, but it inhibited ‘Chojuro’ (S₂S₃) pollen (cross) only slightly. The same unit of RNase T₁ (EC 3.1.27.3) clearly inhibited the pollen tube growth, but the action was significantly weaker than that of the S-RNase against the self-pollen. Inhibitory effect of RNase T₂ (EC 3.1.27.1) and RNase A (EC 3.1.27.5) was only slight. The proteins other than the S-RNase extracted from pear style did not have any inhibitory action, though they possessed RNase activity 3.8 times higher than S-RNase. Thus, RNases tested here could not substitute for the S-RNase in specific inhibition against the self-pollen tube growth. Total RNA degradation by each RNase occurred in the pollen tubes as following order; S-RNase (self) ≥T₁ > T₂ ≥ A > S-RNase (cross). Degradation degree of 28S and 18S rRNA was as follows; S-RNase (self) > A > T₁ > T₂ > S-RNase (cross). The degradation of 5.8S and 5S rRNA was; S-RNase (self) > S-RNase (cross) > A > T₂ > T_1. The degree of rRNA degradation was, thus, not always in parallel with the degree of pollen growth inhibition. The S-RNase may degrade not only rRNA but also mRNA essential for pollen tube growth, and may be specifically adapted to inhibit the growth of self-pollen tubes. Therefore, controlling S-RNase amount in the style will produce self-thinning cultivars efficiently, which are unnecessary not only for hand-pollination but fruit-thinning practices in the pear. Practically, cultivar with weak self-incompatibility and small amount of S-RNase, such as ‘Okusankichi’, may be an expecting candidate for breeding self-thinning cultivars.     

青藏高原草地退化原因述评

导致青藏高原草地退化的因子很多,主要有气候、野生动物和人类活动等。在气候因素中以气温和降水的影响为主,短期内气候的变化不会成为草地退化的主导因素,从长期来看,气候变化与草地退化之间的相互作用可引起草地生态系统的退化;野生动物因素中主要以植食性小哺乳动物的影响为主,其危害程度取决于其种群数量的高低,同时大型野生草食动物对草地退化的影响也不容忽视;人类活动因素中主要以家畜过度放牧为主,在一定程度上,家畜放牧强度的高低直接决定草地的退化程度;草地退化是多种因素综合作用的结果。不同地区导致草地退化的主要因素不尽相同,导致青藏高原草地退化最主要的因子是过度放牧和植食性小哺乳动物种群爆发。针对退化的原因,提出了青藏高原退化草地恢复与管理过程中应注意的事项。     

人类活动对晋西北地区生态环境影响的初步研究

晋西北地处我国北方生态环境脆弱的农牧交错带,同时又是资源富集,资源与环境的矛盾十分突出。人类不合理的经济活动加剧了该区以土壤风蚀沙化和水土流失为主的环境退化问题。因此,加强环境保护刻不容缓。     

Lactobacillus amylophilus JCM1125在生长限制条件下的代谢特性

在以小麦淀粉、玉米淀粉、马铃薯淀粉和可溶性淀粉为碳源代替葡萄糖为碳源的 MRS培养基上检测了菌株 L actobacillus amylophilus JCM112 5活菌数的增殖状况以及葡萄糖和有机酸的代谢特征同时测定了 p H值的变化。结果表明以淀粉为碳源时菌株先分解淀粉成葡萄糖并累积到一定数量后再加以利用 ;活菌数呈现双曲线增长现象 ;丙酮酸、乳酸、乙酸和柠檬酸及另一未知有机酸的数量发生了较大的变化。     

Growth performance and metabolic utilization of diets with native and waxy maize starch by gilthead sea bream (Sparus aurata) juveniles

The effect of dietary starch source and level on growth performance, feed utilization, apparent digestibility coefficients and liver enzyme activities involved in intermediary metabolism of gilthead sea bream juveniles was studied. Five isonitrogenous (47% crude protein) and isolipidic (15% crude lipids) diets were formulated to contain 10% native (diet NS10) or waxy (diet WS10) maize starch; 20% native (diet NS20) or waxy (diet WS20) maize starch or no starch (control). Diets were adjusted with α-cellulose. Another diet was formulated without carbohydrates, and contained 70% crude protein and 15% crude lipids (diet HP). Each diet was fed to triplicate groups of 30 fish (initial weight: 20 g) for 12 weeks. The HP group was fed to near satiation and the other 5 groups were fed on a pair-feeding scheme according to the group that ingested less feed (control diet group). The reduction of dietary protein level from 70% to 47% by the incorporation of 20% starch did not significantly affect gilthead sea bream growth performance or feed efficiency. Compared to the control diet, neither the level nor the nature of starch had any measurable effect on growth performance and feed efficiency. Digestibility of starch was unaffected by source or dietary inclusion level. Diet had no effect on plasma glucose levels, but liver glycogen was higher in diet groups NS20, WS20 and HP. Dietary carbohydrates increased GK and G6PD enzyme activities and decreased ALAT and GDH enzyme activities while had only a minor effect on FBPase activity. The nature of dietary starch tested (native or waxy) had little influence on performance criteria.     

不同施肥水平对马铃薯品质的影响

在平地和坡地条件下,采用4种施肥水平对鄂马铃薯4号品种进行栽培试验,考察不同施肥水平下对马铃薯淀粉品质的影响.结果表明,不同施肥水平下,平地和坡地栽培马铃薯,其淀粉含量达到显著性差异,施低肥时淀粉的含量较高,随着施肥量的增加,淀粉含量随之下降;在相同施肥水平下平地较坡地马铃薯淀粉含量的变化大,说明马铃薯淀粉除受施肥量影响外,还受地形、环境的影响,坡地存在水土流失,导致坡地比平地栽培马铃薯的淀粉含量低0.89%～3.86%.因此,合理栽培、适度开发利用土地资源以及重视水土保持尤为重要.     

Characterization of Degraded Soils in the Humid Ethiopian Highlands

Hardpan is a major cause of land degradation that affects agricultural productivity in developing countries. However, relatively, little is known about the interaction of land degradation and hardpans. The objective of this study was, therefore, to investigate soil degradation and the formation of hardpans in crop/livestock‐mixed rainfed agriculture systems and to assess how changes in soil properties are related to the conversion of land from forest to agriculture. Two watersheds (Anjeni and Debre Mewi) were selected in the humid Ethiopian highlands. For both watersheds, 0–45 cm soil penetration resistance (SPR, n  = 180) and soil physical properties (particle size, soil organic matter, pH, base ions, cation exchange capacity, silica content, bulk density and moisture content) were determined at 15 cm depth increments for three land uses: cultivated, pasture and forest. SPR of agricultural fields was significantly greater than that of forest lands. Dense layers with a critical SPR threshold of ≥2000 kPa were observed in the cultivated and pasture lands starting at a depth of 15–30 cm but did not occur in the undisturbed forest land. Compared with the original forest soils, agricultural fields were lower in organic matter, cation exchange capacity, and exchangeable base cations; more acidic; had a higher bulk density and more fine particles (clay and silt); and contained less soluble silica. Overall, our findings suggest that soil physical and chemical properties in agricultural lands are deteriorated, causing disintegration of soil aggregates, resulting in greater sediment concentration in infiltration water that clogged up macro‐pores, thereby disconnecting deep flow paths found in original forest soils. Copyright © 2016 John Wiley & Sons, Ltd.     

Analysis of mesh breaking loads in cotton gill nets: Possible solution to ghost fishing

ABSTRACT:   A small number of fishers in Chiba Prefecture of eastern Japan use cotton gill nets to catch Japanese spiny lobster Panulirus japonicus. To examine the advantages of cotton gill nets, we analyzed changes in mesh breaking load of a new cotton gill net used in a fishing operation. A new cotton gill net was also soaked in a seawater tank to simulate ghost fishing conditions. The average mesh breaking load of new cotton mesh was 50.3 N. This value decreased to 19.0 N after 38 days (∼912 h), and after 82 days (∼1968 h) the mesh could be easily torn (breaking load 0.07 N). Under fishing conditions, the cumulative soak time was only 744.4 h over 19 months. The average breaking load at the end of this period was 43.1 N, a strength 86% that of the presoaked mesh. The mesh breaking load of a cotton gill net continuously soaked for 744.4 h was 26.1 N, as estimated from tank experiment data. Thus, a cotton gill net maintains reasonable strength under typical use conditions, but will degrade if lost at sea.     

Glycinebetaine Improves Chilling Tolerance in Hybrid Maize

Plant growth and development is hampered by various environmental stresses including chilling. We investigated the possibility of improving chilling tolerance in hybrid maize by glycinebetaine (GB) seed treatments. Maize hybrid (Hycorn 8288) seeds were soaked in 50, 100 and 150 mg l^?1 (p.p.m.) aerated solution of GB for 24 h and were dried back. Treated and untreated seeds were sown at 27 °C (optimal temperature) and at 15 °C (chilling stress) under controlled conditions. Germination and seedling growth was significantly hindered under chilling stress. Moreover, chilling stress significantly reduced the starch metabolism and relative water contents (RWC), and increased the membrane electrolyte leakage. However, activities of antioxidants (catalase, superoxide dismutase and ascorbate peroxidase) were increased under stress conditions. Seed treatments with GB improved the germination rate, root and shoot length, seedling fresh and dry weights, leaf and root scores, RWC, soluble sugars, α‐amylase activity and antioxidants significantly compared with untreated seeds under optimal and stress conditions. Induction of chilling tolerance was attributed to maintenance of high tissue water contents, reduced membrane electrolyte leakage, and higher antioxidant activities and carbohydrate metabolism. Seed treatment with 100 mg l^?1 GB was the best treatment for improving the performance of hybrid maize under normal and stress conditions compared with control and other levels used.