大黄鱼肿瘤抑制因子cylindromatosis (LcCYLD)的序列与免疫反应特征及其功能

为研究大黄鱼肿瘤抑制因子cylindromatosis（CYLD）在免疫反应中的作用,本实验克隆了大黄鱼CYLD的全长cDNA（命名为LcCYLD）并对其进行序列分析;采用荧光定量PCR的方法对大黄鱼各组织及免疫刺激后的大黄鱼肾细胞系中的LcCY LD表达变化进行检测;构建了重组表达载体pTurboGFP-CYLD及pcDNA3.1-CYLD,分别用于亚细胞定位实验及过表达实验;在HEK293T细胞系中过表达LcCYLD后采用双荧光素酶报告系统检测了 NF-κB、TNF-α及IL-1β 启动子活性的变化。结果显示,LcCYLD的ORF包含2 754 bp,编码917个氨基酸,推测具有保守的3个N端的CAP-GLY结构域,1个磷酸化区域和1个C端的UCH结构域,多序列比对结果显示各物种CYLD间高度保守;系统进化分析显示,LcCYLD与来源于其他硬骨鱼的CYLD聚为一支,其中与条纹鲈的CYLD关系最近;转录水平表达分析发现LcCYLD在大黄鱼各组织均有表达,其中在脑中表达量最高;LPS及poly I:C刺激能够显著诱导LcCYLD的表达;亚细胞定位实验表明LcCYLD在细胞质及细胞核中均有表达;过表达LcCYLD能够显著抑制NF-κB及促炎细胞因子TNF-α及IL-1β的转录激活。以上研究结果表明大黄鱼CYLD能够抑制NF-κB的转录激活,为深入了解LcCYLD在大黄鱼先天免疫信号转导中的作用奠定基础。     

Optimal dietary lipid level for large yellow croaker (Pseudosciaena crocea) larvae

A 30‐day feeding experiment was conducted in blue tanks (70 × 50 × 60 cm, water volume 180 L) to determine the effects of dietary lipid levels on the survival, growth and body composition of large yellow croaker (Pseudosciaena crocea) larvae (12 days after hatchery, with initial average weight 1.93 ± 0.11 mg). Five practical microdiets, containing 83 g kg^?1 (Diet 1), 126 g kg^?1 (Diet 2), 164 g kg^?1 (Diet 3), 204 g kg^?1 (Diet 4) and 248 g kg^?1 lipid (Diet 5), were formulated. Live feeds (Artemia sinicia nauplii and live copepods) were used as the control diet (Diet 6). Each diet was randomly assigned to triplicate groups of tanks, and each tank was stocked with 3500 larvae. During the experiment, water temperature was maintained at 23(±1) °C, pH 8.0 (±0.2) and salinity 25 (±2) g L^?1. The results showed that dietary lipid significantly influenced the survival and growth of large yellow croaker larvae. Survival increased with the increase of dietary lipid from 83 to 164 g kg^?1, and then decreased. The survival of larvae fed the diet with 83 g kg^?1 lipid (16.1%) was significantly lower than that of larvae fed other diets. However, the survival in larvae fed the diet with 16.4 g kg^?1 lipid was the highest compared with other artificial microdiets. Specific growth rate (SGR) significantly increased with increasing dietary lipid level from 83 to 164 g kg^?1 (P < 0.05), and then decreased. The SGR in larvae fed the diet with 164 g kg^?1 lipid (10.0% per day) was comparable with 204 g kg^?1 lipid (9.6% per day), but were significantly higher than other microdiets (P < 0.05). On the basis of survival and SGR, the optimum dietary lipid level was estimated to be 172 and 177 g kg^?1 of diet using second‐order polynomial regression analysis respectively.     

大型农业养殖企业控制与管理集成系统研究

首先对CIMS理论及其体系扩展进行分析,并进一步对农业养殖企业控制与管理集成系统的构建统理论和实施进行研究,最后以一家大型农业概念上市公司为例,进行大型农业养殖企业CIMS系统设计的实证研究。     

论两佛线长江穿越复线管道工程

在总结油气管道穿越大型河流工程经验教训的基础上，结合长江上游水深流急工程地质条件复杂的特点，对四川输气南干线黄谦穿越复线工程进行了精心设计施工。穿越断面选在“Ｓ”河道顺直过渡的逆坡河段上，敷设方式采用全断面沟埋，管身结构选用大口径管道装配式重砼加重快，采用特加强外防腐和牺牲阳极保护，施工中采用新技术、新工艺、新设备，确保设计技术要求。与原穿越相比，缩短了穿越管道长度４００ｍ，节省钢材２００ｔ，水泥     

大规格西洋杜鹃嫁接快繁技术

从砧木的采集、处理,接穗选择,嫁接及嫁接后管理等技术,对大规格西洋杜鹃嫁接育苗技术进行总结。     

滴灌成套设备和低压大流量滴头研制与应用

经过10多年的科学研究,我国在微灌设备研制方面取得了一定进展,先后生产试制了微灌用聚乙烯管材与管件,研制出了一批灌水器和过滤、施肥装置,促进了微灌技术的推广运用。对微管灌水器进行的系列化研究,降低了工程造价,提高了灌水质量。     

论大型地下商场空调通风及防排烟

本文通过工程实例说明如何将大型地下商场的空调、通风及防排烟系统三者结合在一起，用一套风系统来实现其各自作用的。     

Study on Large Power Tractor Driver’s Heart Rate and Fatigue in Sowing Work

In order to reduce the driving fatigue in sowing work, this paper based on heart rate (HR) as the main indicator to survey, tested and analyzed the fatigue condition of the drivers of three imported tractors and one domestic tractor in sowing work. The results showed that when driving the imported tractors in sowing work, the HR increasing rate was 10.4%-14.3%, labor intensity belonged to the light level;when driving domestic tractor in sowing work, the HR increasing rate was 23.4%-33.0%, it was remarkably bigger than that of driving imported tractors (P＜0.05), labor intensity belonged to the middle level. The main effects on driving fatigue included the control methods, tractors' cab environment, processing time, operating content, and so on. Finally, we proposed the concrete measures and suggestions to reduce driving fatigue and improve drivers' work condition.     

高大房式仓双层稻壳包覆盖粮面隔热保冷储粮对比试验

利用拌防虫剂的稻壳包对粮堆表面进行双层覆盖,经过三年跟踪试验表明,其隔热保冷效果显著,能减少仓温对粮温的影响,有效地控制粮温上升幅度,特别是表、上层,并且抑制了虫霉的发生,基本保持了储粮原有品质.