香蕉柠檬酸合酶基因MaGCS的克隆及生物信息学分析

采用RACE-PCR的方法首次在香蕉中获得一个柠檬酸合酶基因的cDNA序列。经序列测定和Blastx比对分析表明，该cDNA全长1 885bp，编码513个氨基酸残基，具有植物柠檬酸合酶基因的特征结构域，并与其他植物来源的乙醛酸循环体的柠檬酸合酶具有很高的序列相似性，将其命名为MaGCS(Musa glyoxysomal citrate synthase)，并对其进行生物信息学分析，初步预测其理化性质及功能等。     

Variations in Soil Physical Properties in a Cleared Forestland Continuously Cultivated for Seven Years in Eastern Nsukka,Nigeria

In this study, soil physical properties were evaluated in the top 40 cm of cleared forestland that had been subjected to continuous cultivation for 7 years to ascertain selected crop or crop combinations that influenced the soil physical properties the most. There was no significant effect of crop treatment on particle‐size distributions over 6 years of cultivation. In year 7, clay values were significantly (p = 0.05) greater in plots grown with solely cassava (SC) and solely maize (SM) than in the plots grown with solely pigeon pea (SP). The soil depth effects over the 7 years were significant on the clay content. The mean values of bulk density, pore‐size distribution, and hydraulic conductivity obtained from each plot fluctuated over the years. The bulk density values in 1998 ranged from 1.29 to 1.43 g cm³, but from 1999 to 2004, the range was from 1.12 to 1.40 g cm³. Thus, bulk density generally decreased when compared with their respective values in 1998. The greatest decrease of ≈ 22% was in 2000. More than 70% of the macroporosity values were significantly less than their respective values in 1998. The greatest decease of 72% was obtained from SM plots in 2001. All the microporosity were significantly more than the 1998 values. All the increases were >100% of the original values. These increases were reflected in the variations of total and saturated hydraulic conductivity (Ks) values. However, in 2004, Ks values decreased in the plots grown to C + P, SP, and SM. Generally, the C + M + P mixture appeared to be the most consistent in improving micro‐ and total porosities and Ks among the crop treatments.     

棉籽饼粕在水产养殖中的应用

本文综述了水生动物对棉籽饼粕的应用情况及影响水生动物对棉籽饼粕利用的制约因素.     

Response of Soybean [Glycine max (L.) Merr.] Cultivars to Genistein-Preincubated Bradyrhizobium japonicum: Nodulation and Dry Matter Accumulation under Canadian Short-Season Conditions

The addition of genistein, a plant-to-bacteria signal molecule, to Bradyrhizobium japonicum cells prior to use as inocula has been shown to increase nodule number and promote soybean N₂ fixation at low root zone temperatures. Previous greenhouse and field experiments involving only two cultivars have indicated that soybean [ Glycine max (L.) Merr.] cultivars can vary in their response to genistein application. The objective of this study was to evaluate a range of soybean cultivars for response to genistein application under short-season cool-spring Canadian conditions. A 2-year field study was conducted in 1997 and 1998 with a range of soybean cultivars recommended for Quebec. The 11 cultivars tested represented a range of yield potentials and maturity groups. They were inoculated with genistein-preincubated B. japonicum inocula or regular inocula, applied into the furrow at the time of planting. The results of these experiments indicated that neither maturity nor yield was correlated with increases in nodulation, biomass, and plant total nitrogen content resulting from genistein treatment and that all maturity groups responded to genistein application in essentially the same way. Thus, response of soybean cultivars to genistein addition is regulated by genotype characteristics other than maturity or yield level.     

混合汁中蔗糠含量对沉降和过滤影响的探讨

该文用实验和动态生产试验的方法探讨了蔗糠含量对沉降速度、过滤速度和干滤泥转光度的影响；指出，过量或过少蔗糠含量对这三者会产生急剧干扰；认为，亚法澄清的过滤尚需适量蔗糠作助滤剂等。     

混凝土模板用复合竹碎料板工艺研究

用正交试验法研究了施胶量、热压温度和热压时间3个因素对复合竹碎料板物理力学性能的综合影响．结果表明：热压温度是影响板材物理力学性能的主要因素．较佳工艺参数为，施政量12％，热压温度150℃，热压时间22min，板材物理力学性能能满足混凝土模板材料性能的需要．     

虚拟社区中的网络政治参与的“群体极化”

随着网络技术的飞速发展，虚拟社区已经成为一种人类的“另类空间”，为人类提供一个崭新的交流平台和交往环境。而由于网络的固有的特性，使得网民参与政治以群体主体的形式出现，并且在网络政治参与中表现出“群体极化”现象。群体极化对我国的政治文明建设具有双重影响，全面评价群体极化的影响，探索造成群体极化的深层原因，是引导网络政治群体理性参与政治的基础。引导网络政治群体参与政治，防范群体极化，必须采取综合的措施。     

Supplemental iron and phosphorus increase dietary inclusion of cottonseed and soybean meal in olive flounder (Paralichthys olivaceus)

A long‐term feeding experiment was conducted to investigate the use of cottonseed and soybean meal (CS) with iron and phosphorus supplements in diets for olive flounder (Paralichthys olivaceus). Olive flounder with an initial average size of 28.5 ± 0.35 g (mean ± SD) were divided into 15 groups (three tanks per dietary treatment) and fed 480 g kg^?1 crude protein diets in which each of five isonitrogenous diets was formulated to contain different levels of cottonseed/soybean meal (1 : 1) to replace fish meal (FM) with iron and phosphorus supplementations. The five experimental diets were as follows: diet 1 (control), 0%CS; diet 2, 20%CS; diet 3, 30%CS; diet 4, 30%CS + Fe&P; and diet 5, 40%CS + Fe&P. After 26 weeks of feeding trial, no significant differences were observed in weight gain, feed utilization and survival among all the treatments. The total gossypol accumulation in liver of fish fed diets supplemented with iron was significantly lower than that of fish fed diets without supplementation of iron. The results indicate that the addition of iron in diets could prevent the absorption of free gossypol. The findings in this study suggest that dietary supplements of iron and phosphorus could increase the inclusion of cottonseed and soybean meal for FM replacement in diets for marine fish species.     

多酚羧酸合成功能高分子材料研究(Ⅱ)——没食子酰化改性纤维素合成及其功能特性试验

以没食子酰基为功能基团,以改性纤维素为骨架合成功能高分子材料没食子酰化改性纤维素(没食子酰基-1,6-己二亚胺基-羟丙基-纤维素,CG):先将纤维素与环氧氯丙烷反应,然后依次接上己二胺桥链和三乙酰基没食子酰基,最后将没食子酰基脱去乙酰基得到目标产物。功能试验表明,1g产物能结合明胶49.5mg,并可再生使用。1g产物络合金属离子的能力:Fe3 53.4mg,Cu2 38.7mg,Hg2 46.5mg,Mn2 6.8mg,Zn2 27.9mg,Pb2 57.2mg。产物在稀酸、稀碱、稀醇和热水中具有稳定性。     

沙漠藻固沙结皮的研究与问题探讨

本文综合分析了沙漠藻结皮研究的现状,并结合自己的研究,探讨了沙漠藻固沙结皮的形成与植被发育的关系、沙漠藻结皮人工接种效果,提出了藻沙粒结皮是沙漠藻结皮的早期形式,探讨了进一步研究沙漠藻固沙结皮的关键技术问题。