吉林省粮食生产的结构变动与比较优势分析

在分析吉林省农业生产结构变动过程的基础上，采用显示性比较优势指标，分析了全省粮食生产的比较优势，为全省粮食生产结构调整提供了科学依据，并研究了全省粮食生产结构调整的对策。     

新疆米泉县电力井灌节能节水初见成效

米泉县电力提水灌溉所消耗的能源约占农用动力的80％以上,能源浪费惊人。本文就此问题在机井普测的基础上,采取以综合维修保养为主,并对高扬程的潜水电泵运用变径,变级等改造措施,提出了节水节能的途径,达到少花钱,节能效果显著的目的。     

A Droplet Digital PCR Method for Detection of Newcastle Disease Virus

This experiment was conducted to establish a droplet digital PCR (ddPCR) method for the detection of Newcastle disease virus (NDV). A ddPCR method was developed, which the primers and probes were designed based on the conservative regions of F gene of NDV. The concentration of primer and probe, the annealing temperature in ddPCR reaction were optimized. The sensitivity, specificity and reproducibility of ddPCR method were evaluated. In results, the optimal primer concentration and the probe concentration were 900 and 250 nmol·L^-1, the optimum annealing temperature was 55℃. The detection limit of ddPCR method was 1.8 copies·μL^-1 with a good linear response, it had no cross reaction with other six viruses (include IBV), the coefficient of variation of sample repetition was 2.4%. All the results showed that NDV ddPCR was sensitive and specific, it was suitable for quantitative detection of clinical samples infected with NDV.     

The sand dunes and their vegetation along the Mediterranean coast of France. Their likely response to climatic change

The Golfe du Lion is mainly bordered by low and narrow sand dunes. Since about four decades, 1/3 of its shoreline has been receding, while 1/3 has been prograding and another 1/3 is stable. Several types of dunes may be described mainly depending on storms, high wind frequencies and sand grain size. Vegetation on dune system is distributed along a primary gradient according to sand stability and soil development, and a secondary gradient along slope of dune according to a seasonal cycle of fresh and salt phreatic water level.Global changes in climate may influence these geomorphological and biological structures mainly through:- Winter minimum temperatures changing the distribution of several plant species, especially in the middle part of the Golfe du Lion.- Frequent high storms which cause damages to the front of the dune systems and disrupt the shore. Changes in dune ecosystems will be cyclic so these tendencies will be obvious only upon a long term period.     

老芒麦、垂穗披碱草产草量形成与水热季节变化的关系

两草种产草量季节变化的高峰期一般出现于8月中旬。在此之前的增长动态,符合于 Logistic 生长模式。旺长的始期和终期分别为6月初和7月下旬。这是草种特性在川西北高原水热气象条件作用和制约下的结果,具有明显的地域特征。其中气温的季节变化为主要外因。此外,群体季节生长过程存在着生长中心随生长阶段转换而转移的规律。     

HRP直接标记属特异性基因探针检测沙门氏菌的研究

以活化辣根过氧化物酶复合物（ＨＲＰ—ＰＢＱ—ＰＥＩ＋—ＮＨ３＋）直接标记沙门氏菌属特异性ＤＮＡ探针ｐＬＳ２和ｐＬＳ３，探针与靶ＤＮＡ杂交后催化发光底物，经增强型化学发光反应（ＥＣＬ），用普通Ｘ光胶片自显影（ＣＰＤ）检测沙门氏菌。经狭缝杂交（Ｓｌｏｔｂｌｏｔ），该法标记探针均可检测到０．１ｐｇ的纯质粒ＤＮＡ及１０３个未经培养的鼠伤寒沙门氏菌。Ｄｏｔ—ｂｌｏｔ杂交结果证明，ＨＲＰ标记的探针仅与沙门氏菌属细菌杂交，而与试验的其他肠道非沙门氏菌不杂交。本研究表明，ＨＲＰ直接标记基因探针化学发光自显影法检测沙门氏菌，安全、快速、简便，且有高度的敏感性和特异性，是一种有较大应用前景的非放射性标记探针杂交检测方法。     

猪、禽安全生产中主要病原菌耐药性检测及控制技术研究进展

国内外研究及本课题组的调查表明,猪、禽养殖中主要病原菌的耐药性日趋严重,细菌产生耐药性的机理复杂多样.而药性的检测技术在常规药敏试验的基础上,国内外学者及本课题组已将质粒指纹图谱,PCR,SSCP,核酯探针等分子生物学技术用于耐药性检测.本文还综述和提出了加强兽药管理、制定用药规范、研究质粒消除技术、开发出针对性疫苗、益生素、噬菌体制剂等一系列耐药性的控制技术.     

饲料中娄地青霉(P.roqueforti)的检测

饲料中霉菌及其毒素的污染严重影响畜牧生产和人类健康 ,娄地青霉 (P .roquefortin)是饲料中新近发现的产毒霉菌 ,特别是在青贮饲料中有较高的检出率。其所产毒素对动物有明显的毒性作用 ,值得重视。本试验检测了 1 1 1份饲料样品 ,分别来自德国和中国的猪场、牛场以及鸡场 ,包括玉米、玉米青贮、牧草青贮以及混合饲料和牧草在内的多种饲料。通过霉菌形态学检查 ,对上述样品进行了全面检测 ,确定饲料中霉菌的含量为 2 0 0～ 8 0 0CFU/g饲料 ,青贮料中的优势菌为娄地青霉     

应用酶标抗体染色法实验检定空肠弯曲菌

应用酶标抗体染色法检定空肠弯曲菌,具有较高的特异性及敏感性,除金黄色葡萄球菌呈现轻度着染外(可以从形态学上排除),对沙门氏菌等9种杂菌均不发生非特异性染色反应;用以检查空肠弯曲菌纯培养物,敏感性可达400万个菌/ml。对同时接种10个空肠弯曲菌和1亿个杂菌的30h选择性增菌肉汤培养物,酶染色法可准确地检出,而且不受杂菌的干扰。检查47份鸡粪便标本和38株猪源空肠弯曲菌,其结果与常规细菌分离鉴定结果相同。建立的检定空肠弯曲菌的酶标抗体染色法可在2h内完成。     

板栗树体水分与土壤水分动态变化的相关分析

通过观测板栗的根、枝、叶及栗园土壤水分含量的动态变化发现：根、枝、叶组织水分含量的变化随着栗园土壤含水量的变化而变化；30cm土层为板栗根系的主要吸水层；北方山地栗园，春季每15－20天需要进行一次灌水，以满足板栗树体生长发育对水分的需求。