鸭瘟诊断方法研究进展

鸭瘟是由疱疹病毒引起的鸭、鹅的一种烈性传染病。目前已有多种诊断方法用于鸭瘟的诊断。本文就鸭瘟诊断方法的研究进展作一综述。     

伪狂犬病病毒上海株gE和gI基因的克隆及序列分析

参考Genebank发表的伪犬病病毒（Pseudorabies Virus,PRV）的gI和gE基因序列，自行设计并合成了两对引物，对PRV上海株（PRV－SH）进行PCR扩增，产物经琼脂糖电泳分析，均呈现一条约960bp和1740bp的条带，将其克隆入pGEM-T-easy载体中，进行了序旬测定，将PRV－SH株的gI基因与Rice株gI基因比较发现,核苷酸的同源性为94.7%，氨基酸的同源性为91.3%，证实为gI基因，将PRV－SH gE基因序列与Ea株、Ruce株gE基因序列进行比较，结果显示，该序列与PRV Ea株、Rice株gE基因的同源性分别为98.5%、97.5%；的氨基酸序列与Ea株，Rice株和I型单纯疱疹病毒（HSV－1）17株gE的同源性分别为97.2%、94.8%和15.6%。     

辽宁西部半干旱地区油松纯林改造的研究

喀左县位于辽宁西部地区，现存大面积油松人工纯林，不仅改善地力和水土保持效益差，而且火险性强、虫害蔓延成灾、生长早期衰退。为改善其林分生态条件，提高林木生长量和综合效益，在中三家镇对25年生油松人工纯林进行改造试验。结果表明，选择山杏、刺槐、沙棘等乔、灌木树种，利用林间空地、林缘隙地、侵蚀沟壑和疏林地造林，在大面积油松纯林连片地区进行窄带状或块状砍伐后造林，5年后效益显著，林分相对湿度提高了2．2％，土壤有机质含量提高5．4％，土壤含水量提高9．8％，减轻土壤容重３％,林分虫害明显下降，油松树高、胸径生长量分别提高22．6％、19．8％。     

直接利用传递函数对分叉结构进行模态综合

建立了一种直接利用传递函数对分叉结构进行模态综合的方法。传统的方法一般都需要对大矩阵进行求逆运算,而此方法只涉及分叉结构结合部的传递函数矩阵的求逆,因此求逆计算量很小,特别是当直接采用传递函数的测量值进行模态综合时,就更体现出它的优越性。     

蒙特卡罗模拟信号交叉口的服务水平

信号交叉口是城市路网的节点和交通关键，其服务水平反映了交叉口所提供的道路及控制条件满足交通顺畅、安全等需要的程度。本文提出一种基于蒙特卡罗模拟的信号交叉口运行状态描述和服务水平评价方法．为优化信号交叉口控制参数提供依据．     

用简易诊断法检查判断液压系统各元件的工作性能

故障的排除首先在于判断，目前基层维修网点大多数没有检测仪器和设备，出了故障乱拆乱卸，导致液压元件工作失灵，甚至整车液压系统瘫痪。拖拉机液压系统产生故障的主要原因是液压元件工作性能恶化引起的。为此，介绍了在没有检测仪器和设备的条件下，如何应用简易诊断法检查判断各液压元件的工作性能。     

油研9号氮、钾、硼肥的施用技术

为了探讨油菜良种良法配套栽培技术,以新品种油研9号进行了氮肥的不同施用比例、钾肥的不同施用量、硼肥的不同施用方式的研究。结果表明:在相同施氮量下,以基肥:苗肥:薹肥为3:4:3时产量最高,与5:3:2和6:2:2 的施用比例达显著性差异;与4:3:3的施用比例差异不显著。不同钾肥施用量以底施氯化钾15 kg／667m2产量最高,底施氯化钾均与不施氯化钾达极显著性差异,但底施氯化钾15 kg／667m2比底施氯化钾7．5 kg／667m2增产不显著。不同硼肥的施用方式,以薹施硼肥1 kg／667m2产量最高,薹施(1kg／667m2)、底施(1 kg／667m2)、花喷(100g／667m2)硼肥分别比不施硼肥增产 31．5％、22．8％、5．5％,施用硼肥与不施硼肥均达显著性差异,薹施和底施硼肥增产效果明显,花喷增产效果较差。     

基于Matlab的时序数据两种建模和预测方法比较

利用Matlab工具箱,对时序数据进行了两种不同形式的建模和预测。一是利用系统辨识方法来建立AR模型,利用所建模型进行预测；二是利用单隐层BP神经网络,对数据进行非线性拟合和预测。通过可视化,比较了两种方法的优劣。     

Spatial distribution of Aspergillus flavus and its toxigenic strains on commercial cottonseed from south Texas and its relationship to aflatoxin contamination

The structure of Aspergillus flavus communities associated with south Texas cottonseed was determined by analysing samples from 178 truckloads of commercial cottonseed from 35 gins, extending from Fort Bend County in the north to the Rio Grande Valley in the south, from September 1999 to October 2001. The number of colony-forming units (CFU) of A. flavus on the cottonseed, and the percentage of S strain (%S) were both correlated with aflatoxin contamination of cottonseed. The number of CFU differed between both regions and seasons, while %S differed only between regions. Comparison of maps of CFU and %S revealed that CFU shows a higher variation across years, while %S shows higher spatial variation. The Rio Grande Valley had significantly lower CFU and %S strain than the Coastal Bend and Upper Coast regions. Cottonseed produced in 1999 had significantly more A. flavus than that produced in either 2000 or 2001. Identification of factors dictating geographical variation in S-strain incidence may provide insights that will lead to improved aflatoxin management.     

Influence of death struggle on the structural changes in chub mackerel muscle during chilled storage

ABSTRACT: Chub mackerel (34–35 cm, approximately 500 g), which were caught by fishing with a rod and line at the Bungo Channel, Oita prefecture, were rested overnight in a fish preserve and either killed by decapitation (control group) or allowed to struggle in air for 30 min (struggled group). Muscle samples were excised every 4 h, and measurements on breaking strength and histological observations were done for both groups. The breaking strength of muscle in the control group was significantly higher than that in the struggled group, whereby a decrease in breaking strength was delayed for 12 h compared to the struggled group. Light microscopy showed space extension among muscle cells in association with a decrease in breaking strength. Especially in the struggled group, the extended area was larger and the difference in area was significant at the time when breaking strength showed a significant difference. Using electron microscopy, the extended area showed cut and/or disappeared collagen fibrils. From these results, it was demonstrated that struggling to death promoted the degradation of collagen fibrils and the weakening of connective tissue and, resultantly, led to the faster softening of muscle of chub mackerel.