Plant monocultures produce more antagonistic soil Streptomyces communities than high-diversity plant communities

Plant–soil feedbacks are important to productivity and plant community dynamics in both natural and managed ecosystems. Among soil bacteria, the Streptomyces possess particularly strong antagonistic activities and inhibit diverse plant pathogens, offering a clear pathway to involvement in plant–soil feedbacks. We hypothesized that feedback effects and the ability of individual host plant species to foster antagonistic Streptomyces populations may be modified by the richness of the surrounding plant community. To test this, we collected soil associated with four different plant species (two C4 grasses: Andropogon gerardii, Schizachyrium scoparium; and two legumes: Lespedeza capitata, Lupinus perennis), grown in communities that spanned a gradient of plant species richness (1, 4, 8, 16, or 32 species). For each of these soils, we characterized the potential of soil Streptomyces to antagonize plant pathogens, using an in vitro plate assay with indicator strains to reveal inhibition. We cultivated each plant species in each conditioned soil to assess feedback effects on subsequent plant growth performance. Surrounding plant richness modified the impacts of particular plant species on Streptomyces antagonistic activity; A. gerardii supported a higher proportion of antagonistic Streptomyces when grown in monoculture than when grown in 32-spp plant communities, and L. capitata supported more strongly antagonistic Streptomyces when grown in 4- or 32-spp plant communities than in 8-spp plant communities. Similarly, the feedback effects of particular plant species sometimes varied with surrounding plant richness; aboveground biomass production varied with plant species richness for A. gerardii in L. perennis-trained soil, for L. capitata in A. gerardii-trained soil, and for L. perennis in L. capitata-trained soil. Streptomyces antagonist density increased with overall Streptomyces density under low but not under high plant richness, suggesting that plant diversity modifies selection for antagonistic phenotypes among soil Streptomyces. This work highlights the complexity of feedback dynamics among plant species, and of plant–microbiome interactions in soil.     

橡胶树抗寒种质遗传多样性分析

应用RAPD和ISSR技术对20份橡胶树抗寒种质进行遗传多样性分析。结果表明，16个RAPD引物和12个ISSR引物分别扩增出条带113和101条，多态性条带指数(PPB)分别为61.1%和63.4%。据Nei-Li相似系数，ISSR标记在相似系数约0.74处，可将20份材料分为野生种质和栽培种质两大类；RAPD标记在相似系数约0.70处，也可将供试材料分为野生种质和栽培种质两类，但只有XJ002420为野生种质。对2种标记的分析结果进行相关分析，结果表明，RAPD和ISSR所检测的遗传相似系数呈极显著正相关，相关系数为0.672。以上结论表明，RAPD和ISSR标记可用于橡胶树种质资源的遗传多样性研究，但鉴于ISSR较RAPD有更强的多态性检出能力，ISSR技术应作为遗传多样性研究的首选单引物标记。     

Herbicide savings and economic benefits of several strategies to control Sorghum halepense in maize crops

This study was conducted to assess the herbicide savings and the cost efficiency of site-specific herbicide application strategies in comparison with other strategies based on uniform application of herbicides throughout the whole field. The specific situation considered was Sorghum halepense infested maize fields in Spain. The results from a theoretical economic model were contrasted with the information derived from a S. halepense survey conducted in 37 commercial maize fields distributed over three Spanish maize production areas. Seven application strategies were simulated: 1) no herbicide; 2) overall full-rate; 3) overall half-rate; 4) and 5) site-specific spraying infested cells with full- and half-rate, respectively; 6) and 7) site-specific spraying infested cells plus adjacent buffer areas with full- and half-rate, respectively. The simulation results showed that site-specific weed management was the most profitable strategy when S. halepense infested area ranged between 6.5 and 18.7%. This scenario was present in 22% of the surveyed fields. In fields with less than 6.5% infestation (a situation present in 51% of the surveyed fields), yield losses were slight and the most profitable strategy was using no herbicide. When the infested area ranged between 18.7 and 40.8% (19% of the surveyed fields), no significant differences were observed between the net benefits of the various strategies. Full rate herbicide applications throughout the entire field resulted in the highest net returns in fields with more than 40.8% weed infestation (8% of the surveyed fields). In these cases, the added costs of weed detection, mapping and site-specific herbicide application were not justified by the herbicide savings obtained. Likewise, herbicide savings obtained with the various strategies depended on the proportion of the field infested. Considering the high difference between site-specific treatments and uniform application of herbicides when the infested area ranged between 18.7 and 40.8%, and that no significant differences in net returns were observed in this range, site-specific treatments should be preferred. Adding a buffer area to the site-specific treatments does not seem a suitable strategy due to the considerable increases in costs and reductions in herbicide savings.     

溧阳市生物多样性评价

为溧阳市生物多样性的保护与利用提供依据,以溧阳市生物物种资源普查成果报告和溧阳天目湖森林公园植物资源调查报告为本底数据,采用综合指标体系评价方法对溧阳市生物多样性进行评价。结果表明:溧阳市生物多样性的综合评分为75分,其中物种多样性评分为35分,生态系统多样性评分为40分。溧阳市生物多样性评价结果等级为一般。     

大兴安岭湖通河野生五味子药用资源保护区自然生态质量评价分析

从植被类型多样性、物种多样性、生态系统的稳定性和脆弱性、面积适宜性几个方面对大兴安岭湖通河五味子药用资源保护区的自然生态质量进行了评价和分析。     

藏系绵羊遗传多样性的研究进展

本文系统的论述了藏系绵羊在表型和地域、形态学遗传标记、细胞学遗传标记、生化遗传标记和分子遗传标记等不同方面的遗传多样性研究的概况，说明了藏系绵羊的遗传多样性研究目前仍处于起步水平，因此有必要对藏系绵羊的遗传多样性进行更深入的研究，尤其加强DNA分子水平上的研究。     

Maize Hybrid Variability for Transpiration Decrease with Progressive Soil Drying

Drought is ubiquitous in rainfed cropping systems and often limits maize yields. The sensitivity of transpiration response early in progressive soil drying is a trait with potential to improve crop drought resistance. Simulation studies demonstrated that increased sensitivity to drying soil leading to restricted transpiration rates results in conservation of soil water during vegetative stages for possible use during grain filling. In contrast to other crops, there have been no studies characterizing genotypic variability for this trait in maize. Experiments in controlled environments were conducted to characterize the fraction of transpirable soil water (FTSW) threshold on drying soil for 36 hybrids selected for variation in the field for drought resistance, regions of adaptation and stay green. While FTSW thresholds varied among hybrids from 0.60 to 0.33, these thresholds were not uniformly associated with level of drought resistance in the field. Nevertheless, this study demonstrated a high FTSW threshold corresponded with drought resistance observed in some modern maize germplasm (hybrids #7, 17, 24, 27 and 32). This knowledge can enable breeding work seeking to exploit this adaptive trait to improved drought tolerance in low threshold FTSW germplasm.     

Floristic diversity and vegetation patterns along disturbance gradient in arid coasts in southern Mediterranean: Case of the Gulf of Gabès,southern Tunisia

Knowledge about vegetation patterns along disturbance gradients is essential for conservation and management of coastal habitats in arid regions. This study focuses on the floristic diversity and the factors controlling the vegetation structure in the Gulf of Gabès (Tunisia). For this purpose, the floristic diversity of 120 relevés belonging to three coastal sites with different disturbance levels (40 relevés per site) was analyzed in relation with 10 environmental and anthropogenic factors. Therophytes constitute 54% of the 237 plants species belonging to 44 families identified in the present study. The species richness and floristic diversity indices exhibited a similar trend and peaked in the slightly disturbed site (H′?=?3.38; S?=?176). The similarity between sites was relatively low and reached its maximum between the little and the highly disturbed sites. Two-way indicator species analysis and detrended correspondence analysis applied on the floristic data resulted in groups mainly determined by disturbance level by explicitly segregating the three sites. Canonical correspondence analysis (CCA) indicated that the spatial pattern of species composition was mainly influenced by anthropogenic variables. Partial CCA revealed that the effect of anthropogenic variables on floristic composition was thrice higher than the effect of environmental variables (57.5% vs. 18.4%, respectively). Overall, this study provides a broad understanding of the floristic diversity and vascular plant richness in the Gulf of Gabès. The obtained results reflected a disturbance–diversity pattern and thus revealed the obvious importance of maintaining the intermediate disturbance for biodiversity conservation and monitoring.     

抚顺西排土场矸石山阴坡物种多样性变化特征

抚顺西排土场矸石山阴坡随着排矸年限的增加，物种多样性发生变化，物种数由初期的4科5种增至后期14科29种。植被由最初的鹅绒藤＋豚草＋刺蒺藜群落，逐步演变到榆树群落。在阴坡，不同排矸年限的样地，植物群落多样性指数随着排矸时间的延长而增加；物种综合多样性指数、丰富度指数与排矸年限呈显著正相关关系。     

陇东地方牛种遗传多样性研究

对陇东地方牛种及其与秦川牛和南德温牛杂交群体共计113个个体线粒体D-loop区461 bp序列进行多态性分析,结果表明,3个群体该区域A+T含量(64.2%)明显高于G+C含量(35.8%),表现出明显的碱基偏倚;3个群体核苷酸变异率介于0.42%和3.27%之间,陇东牛和南德温牛杂交群体核苷酸变异率最高;核苷酸变异类型以转换为主,转换占核苷酸多态位点的90.94%,其次为颠换,占4.59%,同样表现出明显的碱基替换偏倚;3个群体核苷酸岐异度介于0.008 95和0.029 06之间,遗传距离介于0.000 15和0.008 36之间,陇东地方牛及其与秦川牛的杂交群体核苷酸歧异度和遗传距离最小。