Determinants of groundnut rosette virus disease occurrence in Uganda

Groundnut rosette virus disease (GRVD) is the major constraint to groundnut (Arachis hypogaea) production in Uganda. It is principally transmitted by the groundnut aphid (Aphis craccivora Koch). The disease is known to cause total crop failure in cases where susceptible varieties are used. During any particular season, GRVD displays variations in incidence and severity in different agro-ecologies within the country, but the reasons for the varying disease patterns remain unclear. This study was aimed at establishing the factors influencing the occurrence of GRVD in Uganda. Trials were established for three seasons in four groundnut growing locations situated in different agro-ecologies in Uganda. Four groundnut genotypes were used as treatments in a randomized complete block design with four replications. Disease progress and aphid populations were assessed at 4, 8 and 12 weeks after planting. Data on environmental factors; particularly rainfall, temperature and wind speed were obtained from standard meteorological stations located at/near the study sites. Soil samples and yield data were also obtained in each season. The study revealed that disease incidence; severity and groundnut yields were significantly affected by season, location and genotype. The same applied to their three way interactions. Levels of disease infection were found to be majorly influenced by rainfall and wind speed. Disease incidence and severity were generally higher in conditions with less rainfall and low wind speeds. The Pearson's two tailed correlation between total rainfall and disease incidence for all trial sites was negative and highly significant (r = −0.280, P ≤ 0.01). The same was true for wind speed and disease incidence (r = −0.476, P ≤ 0.01). However, there was no conclusive trend between temperature and disease incidence with the Pearson's two tailed correlation showing significantly positive and negative trends depending on location.     

An improved method for extraction of sorghum polymeric protein complexes

A method for fractionating sorghum proteins using extraction solvents and techniques designed to obtain polymeric protein structures (especially disulfide linked) was developed. Extraction and separation conditions were optimized in terms of completeness of protein extraction, sample stability, and analytical resolution. After pre-extraction of albumins and globulins, a 3-step sequential procedure involving no reducing agents was applied to ground whole sorghum flour. The three fractions obtained represented proportionally different protein polymer contents and molecular weight distribution as evidenced by comparative size exclusion chromatography. Protein composition also varied among the extracts with differences in kafirin composition and non-kafirin proteins detected in the fractions by RP-HPLC and SDS-PAGE analysis. The ability to quantify and further characterize sorghum polymeric protein complexes will be useful for additional studies linking protein structures with functionality and digestibility and variations for these properties within diverse sorghum germplasm.     

基质分散萃取-高效液相色谱法对菠菜中噻虫胺残留的检测

研究确立了乙腈为分散萃取溶剂,以羧基化双壁碳纳米管(DWCNTs-COOH)和石墨化碳黑(GCB)为基质分散材料的菠菜(Spinacia oleracea L.)样品中噻虫胺残留基质分散萃取前处理方法,建立并优化了乙腈等度洗脱的HPLC残留样品外标定量方法。结果表明,优化色谱条件下,噻虫胺标准样品的色谱保留时间为6.258 min,在0.05~100 mg/L范围内与对应色谱峰积分面积线性响应良好,回归方程为y=4.948 2x-1.316 9(R~2=0.999 7),噻虫胺在菠菜样品中0.1、0.5和5.0 mg/kg 3个水平的添加回收率均在85%以上,各添加水平的相对标准偏差均小于7%,该色谱条件下仪器检出限为0.052 7μg/L,方法的最低检测量为0.015 mg/kg。表明该残留样本前处理方法和样品检测方法简便、高效、经济、可靠,可满足噻虫胺在菠菜中的残留定量检测要求。     

羊高频高效繁育调控技术应用现状和操作要点

阐述了羊高频高效繁育调控技术的国内外研究应用现状,并结合生产实践总结出羊高频高校繁育调控技术操作程序及要点,其内容包括：羊的同期发情、多胎技术、精液解冻及镜检、输精前处理、腹腔镜观察卵巢状况、腹腔镜子宫内输精、早期妊娠诊断、羔羊代乳粉、羔羊早期断奶等技术和方法。     

论西方音乐本质观的三种模式及其演变规律

在西方音乐美学史上，出现了三种音乐本质观的模式：即以柏拉图和亚里斯多德为代表的古典时期的模仿论，以黑格尔和李斯特为代表的浪漫时期的表现论和以汉斯立克等为代表的现代时期的形式论。每一种模式的出现都扎根于时代的思潮和哲学思想的转变，三种模式的演变，代表了人类对音乐本质特征的深刻认识，表征着人类对音乐的本质认识的深度和广度。对此研究对我们今天中国的音乐教育和音乐理论建设有一定的启示作用。     

环境管制政策的实施效果评价——以九龙江流域"限猪令"为例

环境管制一直是我国环境污染治理所采取的主要政策之一,对我国的环境保护起到了一定的积极作用,同时也存在一些弊端,致使其成效不甚显著甚至背离初始目标.在此情况下,如何评价环境管制政策绩效具有重要的理论和实际意义.本文以九龙江流域"限猪令"政策为例,选取政策目标实现度、主要环保指标实现、环保投入效益、公众参与及满意度四个方面...     

国家重点实验室专项经费绩效预算管理方式应用于其他财政专项管理的思考

绩效预算是我国部门预算管理改革的重要内容。国家重点实验室专项经费绩效预算安排机制，是财政科研经费绩效预算的典型示例。文章通过剖析国家重点实验室专项经费绩效考评管理机制，对国家重点实验室绩效预算机制推广应用于其他财政专项的范围、考评指标、考评工作方法、考评结果应用等方面提出了意见建议。     

砂光机导向辊动态性能的临界转速计算与分析

通过对砂光机导向辊动态性能的临界转速分别进行传统计算和有限元分析验证,分析两者之间的差别以及产生误差的原因,从而对导向辊的设计及合理使用提供理论依据。     

设施农业高效用水信息化发展及其技术体系探讨

发展设施农业用水信息化是提高水资源利用效率与效益,实现水资源优化配置的重要途径。在分析国内外设施农业用水信息化发展现状的基础上,结合信息化发展的需求,研究了设施农业高效用水信息化的技术体系,重点分析了基础数据的监测网络及共享平台、作物需水信息评价与检测、输配水系统的构成、功能及工作过程,旨在为提高设施农业用水信息化水平提供决策依据。     

以“德”抗“位”的回归——论孟子、赵岐和朱熹的君臣观

在君臣关系上，先秦孟子主张德高于位，东汉赵岐虽未能继承孟子衣钵以德抗位，却也守道不违，守住了人臣的基本原则。而至南宋朱熹在吸纳与反省宋代《孟子》议题的时候，再次开掘和彰显仁义道德本真，以德抗位的人臣之本也自东汉赵岐之后得以回归。