河北二季作区马铃薯节水灌溉研究初报

为研究河北二季作区马铃薯整个生育期不同灌水量对其生长发育的影响,以该地主栽品种为材料,根据不同生育期需水规律,设定5个不同水量梯度的滴灌处理和1个常规沟灌处理(CK);调查分析了不同处理间物候期、重要农艺性状、病害发生情况和产量等相关指标。结果表明,处理3(采用滴灌模式,水量为110 m3)与常规沟灌相比,马铃薯生长势、总产量和商品薯产量无显著差异,生育期提前,病害较轻,商品薯率提高7.2%,节水35.41%,综合表现优于其他处理,为试验地区最佳灌水量。     

剪叶对春油菜源库关系的影响

试验以春油菜青油-46为供试材料,在花期进行剪叶处理,研究源的减少对库器官的分化和形成的影响,研究表明:油菜花期去叶减少了源的面积,影响了油菜光合产物的积累,使油菜株高降低,茎秆变细,分枝减少,角粒数下降,千粒重增加,角果大小、单位角果皮面积的生产力(PPA)、单位角果皮面积负担的籽粒数(SNPA)下降,生物产量和经济产量降低,对分枝产量的影响大于主花序,且去叶越多下降越大,其中去短柄叶的影响大于长柄叶。     

宁夏旱作农业区应用全膜双垄沟播技术的效应分析

摘要：通过对宁夏旱作农业区应用全膜双垄沟播技术效应、存在问题的分析，肯定了该项技术是旱作农业的一项突破性技术，针对其在生产应用中存在的技术问题，提出了提高认识、强化技术培训、提高技术到位率、加强技术创新力度、坚持地膜补贴惠民政策、建立旧膜回收机制、加强领导等对策，以加快全膜双垄沟播技术的应用速度，发挥更大的增产、增效作用。     

大口径长输管道用弯管的设计制造

在国内外缺少适用于大口径长输管道用的大曲率半径的弯管设计、制造规范的情况下，建议在符合现有规范的条件下设计时，应考虑曲率半径的选取尽可能小，以减小所需变制设备、芯模的规格尺寸和空间场地；当弯管和与之相连的直管材质不同时，应在壁厚计算公式中乘以两种不同材质的屈服强度比值；为节省投资和合理利用钢材，应慎重确定弯管制造的壁厚减薄量；为保证弯头质量，在选材上不宜选用螺旋焊缝钢管，而应选用高强度低合金钢管或     

Resistance to early blight of tomato with respect to various parameters of disease epidemics

Resistance to early blight in the tomato was assessed by examining various parameters of the progress of the disease. Artificial inoculation and the scoring technique were standardized. Test plants were inoculated with 125cfu/ml of a 12-day-old culture of a pathogenic isolate of Alternaria solani. Screening under artificial conditions was more informative than that under natural epidemic conditions. Tomato cultivars CLN-2071-C, CLN-2070-A, BSS-174, and DTH-7 with resistance expressed as slow blighting against four pathogenic isolates of A. solani, were selected for cultivation in disease-prone areas. Disease intensity increased with the age of plants under the same inoculum load. The area under the disease progress curve (AUDPC) was positively correlated with the percentage disease index and negatively with resistance. Calculation of the apparent infection rate (r) was more informative for natural epidemics than for artificial conditions. The sequential apparent infection rate between observation periods was better correlated with disease progress than was the total apparent infection rate between the first and last observations. A double sigmoidal disease progress curve during the same cropping season was characteristic of some varieties when fungal infection took place during the vegetative phase of crop growth.     

Double in situ hybridization for simultaneous detection and differentiation of porcine circovirus 1 and 2 in pigs with postweaning multisystemic wasting syndrome

Double in situ hybridization using a digoxigenin-labelled porcine circovirus 1 (PCV1) and biotinylated PCV2 probe, was developed for the simultaneous detection and differentiation of PCV1 and PCV2 in formalin-fixed, paraffin-embedded tissues from pigs with postweaning multisystemic wasting syndrome. The combination of an alkaline phosphatase conjugated antidigoxigenin system with alkaline phosphatase conjugated streptavidin-biotin system allowed identification of PCV1 and/or PCV2. No evidence of cross-reaction was observed. Positive cells exhibited a red or dark brown reaction product for PCV1 and PCV2, respectively. Both PCV DNAs were observed mainly in the cytoplasm but occasionally in the nucleus. Co-localization of hybridization signal for both PCV1 and PCV2 was present in macrophages and multinucleated giant cells of the lymph node and spleen. This double-labelling technique for the differentiation between PCV1 and PCV2 is suitable for pathogenesis studies and diagnostic applications.     

奶牛附红细胞体和伊氏锥虫二重PCR诊断方法的建立

为建立奶牛附红细胞体和伊氏锥虫两种病原诊断方法并探索二者之间在奶牛感染中的关系,本研究针对两病原分别设计两对特异性引物,建立了奶牛附红细胞体和伊氏锥虫感染的二重PCR诊断方法,其扩增片段大小分别为415bp和237bp。敏感性试验和特异性试验表明,附红细胞体和伊氏锥虫的DNA的最低检测量为0.154pg和0.105pg;与猪肺炎支原体、大肠杆菌、葡萄球菌、鸡艾美耳球虫、牛双芽巴贝斯虫无交叉反应。35份临床血样检测结果为:奶牛附红细胞体阳性率22.89%,伊氏锥虫阳性率8.89%,其中共感染率为2.89%。临床试验表明,该方法可用于奶牛附红细胞体和伊氏锥虫的诊断,特别适用于早期诊断。     

蘑菇培养料加入864菌液发酵与二次发酵的效果比较

通过加入８６４菌液发酵和二次发酵栽培蘑菇的试验,比较培养料中微生物群落、培养料成份及产菇量,说明加入８６４菌液发酵是代替二次发酵栽培蘑菇的较好方法。     

白菜型油菜双显性核不育896AB恢复系基因型的鉴定

以白菜型油菜双显性核不育896AB为材料，采用成对兄妹交和相应可育株自交，验证显性不育基因的遗传;用恢复系与全不育系测交，测交一代与临保系复交，验证显性恢复基因的抑制作用，并区分F2代育性分离为3:1和13:3的遗传类型。经4个年度的研究认为，育性是由一对显性不育基因MSMS和一对显性可育基因RfRf互作控制，且显性可育基     

基于倍四元数和Groebner基的6R机械手的位置反解

提出了一般6R机械手的位置反解的新算法,该算法是把齐次变换矩阵转以倍四元数形式表示,建立了倍四元数形式的串联6R机械手运动学方程,再通过线性消元和两次应用分次字典序Groebner基消去5个变元,得到一个一元16次方程.此方法没有增根,是一种对空间任意尺寸6R机械手进行建模和求解的新算法.其中求解Groebner基采用了计算机代数系统进行有理数运算,目前的求解速度尚不是很高,需进一步改进.