Segregation of 12 isozyme genes among doubled haploid lines derived from a japonica x indica cross of rice (Oryza sativa L.)

Summary The segregation of 12 heterozygous isozyme markers was analyzed among F₂ plants and 51 anther culture (AC)-derived lines obtained from the japonica × indica cross of rice, IRAT 177 × Apura. All the lines except two were homozygous products of recombination of the two parental phenotypes. Doubled haploid (DH) lines derived from plants regenerated from the same callus were identical, confirming previously obtained results in rice. Surprisingly, some lines derived from different calli were also identical, suggesting a phenomenon of early callus fragmentation. All these observations at the isozyme level were confirmed by field evaluation. Deviations of segregations from the expected 1 : 1 ratio were observed at 4 loci among the DH lines. Among these, two were also noted among the F₂ plants. The two other distortions, both in favor of the japonica allele, were observed specifically in the AC-derived materials.Although this concerns a small proportion of the genes under study, it suggests that the embryogenic microsporal population does not represent a random gametic array. On the other hand, evaluation of recombination between isozyme genes located on chromosome 6 appears consistent with F₂ data and data previously recorded on the other japonica × indica crosses. The potential use of isozymes in breeding doubled haploids derived from remote crosses in rice is discussed.Abbreviations MCPA = 2-methyl-4-chlorophenoxyacetic acid - IAA = indolacetic acid - AC plant or line = anther culture-derived plant or line - DH line = doubled haploid line     

Evaluation of a simple technique for characterizing rice root systems in relation to drought resistance

Summary Drought is a constraint to rice yields in much of Asia's rainfed rice crop. Although genotypic variation for root system characteristics is well documented in this semiaquatic species, little improvement of rice for drought resistance has been attempted. No simple method to evaluate root systems exists. In this paper we report the development and testing of a simple device to measure the pulling force required to uproot rice seedlings. The force required to pull rice seedlings from the paddy soil was correlated with root weight, root branching and thick root number. The capability to separate rice cultivars by this technique and relationship of the root pulling force to other drought screening methods are illustrated.     

空气温湿度对水稻灌浆及空壳率的影响研究

对1996—2005年10个年份的晚稻孕穗至成熟期的空气温度与相对湿度对水稻空壳率和灌浆影响进行了研究，结果表明：相对湿度对水稻的灌浆速度没有显著影响；灌浆期气温的升高对水稻灌浆速度的增加有显著影响；日最高温度大于或等于30℃的天数对水稻秕粒率的影响是不显著的；但孕穗期至抽穗开花期日最高温度大于或等于30℃的天数对水稻空壳率的影响是极显著的。最后对防御高温危害提出了建议。     

转玉米C4光合酶基因水稻株系中的光合C4微循环

用转PEPC、PPDK、NADP-ME、PEPC PPDK等酶基因的水稻株系及原种野生型(WT)为材料, 研究了不同基因型水稻叶片中的C4光合微循环及其功能.用外源OAA或MA饲喂叶切片或离体叶绿体后,光合放氧速率在野生型水稻中增加了50%,在NADP-ME-和PPDK-转基因水稻中增加了50%～54%,在PEPC-和 PEPC PPDK-转基因水稻中增加了100%～150%.证明原种水稻Kitaake叶片中具有一个原初的和有限的C4光合微循环,除PPDK基因、NADP-ME基因外,外源PEPC基因或PEPC PPDK双基因导入原种水稻Kitaake后,可大幅度提高C4光合微循环的运行.水稻中C4光合微循环的增强有降低光呼吸速率(Pr)、增加净光合速率(Pn)的作用,在光能利用上,可增加PSⅡ光化学效率(Fv/Fm)、光化学猝灭(qp)、降低非光化学猝灭(qN)的作用;这些结果为转C4光合酶基因水稻中建立C4微循环系统来提高光合作用效率的可能性提供了依据.     

关于水稻穗芽的生理学研究

利用重组自交系籼稻中-156/谷梅2号304份株系为材料，在田间条件下营造高温、高湿的小气候，以“穗穗芽率”及“粒穗芽率”为指标，筛选易发生穗芽与不易发生穗芽的极端材料，比较研究重组自交系亲本与各供试株系籽粒在穗芽发生期间的生理差异。结果表明，该重组自交系母本谷梅2号比父本中-156易穗芽，在正常成熟过程中籽粒内源GA₁含量水平高于中-156，ABA含量水平低于中-156；谷梅2号籽粒淀粉酶活性也高于中-156。花后15 d至完熟的籽粒灌浆期如遇高温、高湿条件，谷梅2号及易穗芽的株系籽粒内源GA1随雨日渐增，增幅高于中-156及不易穗芽的株系，同时其淀粉酶活性回升，在正常成熟过程中花后15 d后籽粒中淀粉酶活性与日渐降，但其幅度远高于父本中-156及不易穗芽的株系，这种变化可能是易穗芽的亲本与株系对高温、高湿敏感诱发穗上发芽的主要生理基础。     

精量播种条件下冬小麦的高产优质初探

为了研究冬小麦在小播量条件下的产量和品质表现，2001～2003年在北京安排了两个年度的秋播试验。参试品种有DS1号、临抗1号、冀麦30、鲁麦21和京411，京411是对照品种，播种量是22．5kg／hm^2。2001-2002年度，参试品种中临抗1号的最高产量达到6652．5kg／hm^2；2002-2003年度中，京411、鲁麦21、临抗1号、DS1号、冀麦30的平均产量分别达到8537．6，7403．7，7353．75，6836．25，6169．8kg／hm^2，品种的品质也较好。试验中，这些品种有正常的生育表现，较强的分蘖能力，旺盛的生理活动。在产量结构中，这些品种的单株穗数较多。     

水稻白叶枯病抗性基因的聚合及其遗传效应

以全生育期抗水稻白叶枯病的早粳Ｇ３８为抗源供体，以成株期抗性的晚粳品种秀水１１为轮回亲本，通过连续回交转育出全生育期抗白叶枯病的晚粳品系Ｄ６０１、Ｄ６０２和Ｄ６０３。遗传研究表明，３个晚粳品系都聚合了来自双亲的ｘａ－５和Ｘａ－３抗性基因，聚合的抗性基因可能具有抗性累加效应，其抗性水平和抗扩展能力强于双亲，抗谱宽于秀水１１。同时评价了３个晚粳稻抗病品系对我甸白叶枯病病原型的抗性及主要农艺性状。认为Ｄ     

福建早稻产量结构要素的气象影响研究

通过对福建早稻多年田间观测数据与气象资料的相关和回归分析,研究气象条件对早稻产量结构要素的影响,结果表明穗结实粒数是造成早稻产量年际变化的主要因素,幼穗分化到乳熟期的光温水条件对早稻产量起着主要作用。     

优质早籼稻产量性状分析及育种策略探讨

对115份优质早籼稻新品系的产量性状与产量的相关分析和通径分析结果表明：各产量性状均与产量呈正相关,除株高外,其余5个性状与产量的相关均达极显著或显著水平,产量性状之间存在着相互制约和相互依赖的关系;每穴穗数对产量的贡献最大（47.84%）,其次为每穗总粒数（21.86%）,再次为千粒重和结实率（分别为14.47%和9.46%）.根据分析结果,笔者认为优质早籼稻高产育种应主攻分蘖和提高分蘖成穗率,同时注意提高每穗粒数和千粒重.优质早籼稻高产（即产量达500～600kg/667m^2）育种的产量性状模式为单株有效穗8～10穗、每穗总粒数125～150粒、结实率80%以上、千粒重24～27g.     

稻壳粉一次性筷子制备的试验研究

以稻壳粉为基材,以羧甲基纤维素钠和黄原胶为黏合剂、色拉油为脱模剂,利用正交试验找出最佳比例的配方。结果表明,最佳比例的配方为稻壳粉154.7 g,羧甲基纤维素钠7.65 g,黄原胶7.65 g,检测证明一次性可降解的稻壳粉筷子综合性能良好。