耐辐射球菌抑菌圈试验过程中出现的问题与分析

[目的]分析耐辐射球菌抑菌圈试验过程中出现的问题并提出解决方法。[方法]采用已发表的基因组序列作参考,对来自于耐辐射球菌的所有菌株均用TCY培养基或者TGY平板在30℃条件下进行培养,参照Debabrota等的方法进行抑菌茵圈试验,所用突变体源于以前的试验。[结果]分析认为,在做耐辐射球菌抑菌圈试验的过程中,首先需要注意的是严格灭菌,每次重新拿到超净工作台上的离心管和钢圈都要进行严格的灭菌处理。试验前对上清液要进行高速离心,以防止在上清液中混有菌体,从而造成结果不准确。最好在进行抑菌圈试验以前,先对上清液中是否含有菌体做一个前期试验,以免造成后面不必要的麻烦。[结论]该研究所提出的解决方法对做好以后的试验具有借鉴意义.     

气缸套变形与活塞环摩擦功关系的研究

为了降低发动机摩擦损失,提高机械效率,重点研究缸套变形对活塞环与气缸套摩擦副的影响。首先,对两种不同状态的缸套进行有限元分析,找出缸套变形规律;然后,根据这些规律计算出活塞环与缸套润滑表面的油膜压力和油膜厚度;最后,将计算结果与试验结果进行对比,验证了该方法的正确性。研究结果表明,缸套变形的椭圆度直接影响摩擦功和机油消耗率的大小。     

豇豆轮纹病病原鉴定及其室内药剂筛选

从海南三亚豇豆设施棚内采集具典型轮纹病病斑的豇豆叶片,采用组织分离法分离到1株致病菌,根据病害症状特点、病原菌形态特征、培养性状、ITS序列分析,鉴定豇豆轮纹病的病原菌为多主棒孢霉[Corynespora cassiicola (Berk. & Curst.) Wei];利用生长速率法,对12种药剂进行室内筛选,结果表明：10%苯醚甲环唑(WP,可湿性粉剂)和50%异菌脲(WP)对病原菌具有强烈抑制作用,其EC50分别为2.709 46和9.093 27 mg/L,而75%百菌清(WP)和50%多菌灵(W     

环鄱阳湖地区生态旅游开发研究

分析了环鄱阳湖地区生态旅游开发的优势以及鄱阳湖旅游业的发展现状,并在此基础上提出了环鄱阳湖地区生态旅游开发的对策。     

Automatic counting of annual rings on Pinus sylvestris end faces in sawmill industry

One of the essential requirements of current agricultural practice is the need to evolve towards crop techniques that make better use of productive factors behaving in sustainable and environmentally respectful ways. Pest control is one of the most important problems to take into account, due to the significant production losses that pests may cause. The Integrated Production for olive crop defines a set of rules that has to be followed in order to ensure a production of higher quality, and promotes an environmentally respectful model of Olive cultivation. This work presents SAIFA (spanish acronym for Sistema de Alerta e Información Fitosanitaria Andaluz - Andalusian Phitosanitary Information and Alert System) a web-based information system which allows monitoring the Integrated Production for the olive crop in Andalusia, Spain. SAIFA has been built as a tool to assist agricultural technicians to comply with the quality standards of Integrated Production, and to help decision makers to choose the actions to be performed in the crop. Also, it assists coordinators to choose an Integrated Production strategy applicable for the whole region with the main objective of ensuring the safety of crops and reporting the current phitosanitary state to the authorities.     

Factors influencing the association between active ingredient and adjuvant in the leaf deposit of adjuvant-containing suspoemulsion formulations

BACKGROUND: For an oil adjuvant to enhance uptake of a particulate active ingredient (AI), it is hypothesised that closer association between the two should result in higher uptake. Accordingly, factors important for the spray deposit size on grapevine leaves have been investigated for a series of model suspoemulsion formulations containing colloidal crystalline AI or fluorescent pigment particles and an emulsion of an oil adjuvant with different degrees of wetting and different spray volumes. RESULTS: Low spray volumes (<100 L ha(-1)) produced small deposits with high particle-adjuvant association. Complementary uptake studies showed increased uptake with decreasing deposit size, in agreement with the above hypothesis. Higher spray volumes produced larger deposits that consisted of annuli formed by pinning of the contact line by particles. Low surfactant concentrations favoured particles in the annulus and adjuvant separated in the centre. Intermediate surfactant concentrations produced annuli containing both particles and adjuvant, while with high surfactant concentrations the deposits were large with few annuli.CONCLUSIONS: Small deposits result in high AI-adjuvant association. With larger deposits, annulus structures allow for enhanced AI-adjuvant association (5-20 times greater). The formation of annuli appears to be important in enhancing the biodelivery of particulate AIs in adjuvant-containing suspoemulsion formulations at intermediate spray volumes.     

Selenium supplementation changes glutathione peroxidase activity and thyroid hormone production in Senegalese sole (Solea senegalensis) larvae

Dietary selenium (Se) influences the glutathione peroxidase (GPx) activity and the thyroid hormone system in vertebrates, but these relations are poorly described in fish. The present study aimed to evaluate the effect of Se supplementation on Senegalese sole larvae, focusing on GPx activity and thyroid status. Two groups of larvae were fed on either control (C) or Se‐enriched live feed, from 2 days after hatch (DAH) until 34 DAH. Senegalese sole larvae fed Se‐enriched live feed significantly increased their whole‐body Se, compared to larvae from C group. The activity of GPx was higher in larvae from Se group, suggesting that the levels of this mineral were suboptimal in the control diet. TH levels were also higher in larvae fed Se‐enriched live feed, suggesting that Se may have a positive influence on the TH production in early life stages of marine fish larvae.     

不同贮藏温度对玉露香梨采后生理和贮藏品质的影响

以玉露香梨为试材,分别研究了三种贮藏温度（-1．5℃、0℃和7～－2℃）对果实采后生理和贮藏品质的影响。结果表明,-1．5℃近冰温贮藏比O℃贮藏能更好地降低果实腐烂率,减少果心褐变的发生,推迟呼吸高峰的到来时间,延缓硬度、可滴定酸含量下降,减缓果肉相对电导率升高．减少丙二醛生成和果心中酚类物质含量．维持较高的超氧化物歧化酶活性．但对过氧化物酶和多酚氧化酶活性影响不明显。-1．5o（=和O℃冷藏与土窑洞贮藏（7～－2℃）相比．除对果实可溶性固形物含量（SSC）的影响差异不显著外．其他采后生理与品质指标均显著优于后者。说明适宜的低温可以提高玉露香梨贮藏品质,延缓衰老。九成熟的玉露香梨在7－．2℃土窑洞条件下贮期为120d左右,O℃及近冰温－1．5℃下贮期可达240d左右．但近冰温贮藏的果实品质明显优于0℃冷藏。     

IMAGING DIAGNOSIS—USE OF RADIOGRAPHY,COMPUTED TOMOGRAPHY,AND ENDOSCOPY IN THE DIAGNOSIS OF ESOPHAGEAL PERFORATION BY AN EXTRA‐LUMINAL TRACHEAL RING PROSTHESIS IN A GOLDEN RETRIEVER

A 6‐year‐old neutered male, Golden retriever presented with regurgitation and excitement‐induced coughing. Two years prior, extra‐thoracic tracheal collapse was diagnosed and extra‐luminal tracheal ring prostheses were placed. Radiographs at presentation showed mineralized and undulant cervical tracheal cartilages. A dorsally broad‐based soft tissue opacity bulged into the caudal cervical tracheal lumen. Esophageal dysmotility and barium accumulation were identified at this level on contrast esophography. Computed tomography showed migration of a prosthetic tracheal ring, resulting in perforation of the esophagus. Hair, foreign material, and dynamic tracheal narrowing were identified on esophagoscopy and tracheoscopy, respectively. Esophagostomy and foreign body removal were performed.     

不同工艺参数下苜蓿草粉环模制粒机流场的模拟与验证

环模制粒成型技术以其高效率、高成型率、强适应性等优点广泛应用于生物质能源和饲料产业等领域。该文以苜蓿草粉为原料,应用POLYFLOW软件对环模制粒机挤压区流场进行数值模拟,研究了喂料量、环模转速和物料含水率的变化对流场压力、速度、剪切速率和黏度分布特性的影响规律,并以试验验证,旨在为生产工艺的有效控制提供参考依据。结果表明:在结构参数等条件一定的情况下,增大喂料量,流场压力升高,挤压成型区域扩大,且物料以较快的速度作层流运动,流场剪切速率降低,黏度增大,出模压力和成型密度较大;减小环模转速,流场压力和挤压成型区增大,流动速度减小,剪切速率降低,黏度较大,但出模压力和成型密度降低;物料含水率降低使流场压力、黏度、出模压力和成型密度增加。通过比较得出:当喂料量为6 t/h、环模线速度为6.5 m/s和物料含水率为15%时所形成的流场有利于苜蓿草粉的制粒成型。