玉米秸秆对土壤微生物性质的影响

以黑钙土为供试土壤，初步研究了玉米秸秆对黑钙土微生物数量及种群丰富度、种群更替的影响。180d的培养试验结果表明：玉米秸秆对土壤微生物的数量、活性具有促进作用。其中，玉米秸秆对土壤真菌数量的影响大于对细菌数量的影响，细菌与真菌的数量比（B／F）由1.42降低到1.25。微生物种群丰富度下降，具有使土壤微生物区系由细菌型向真菌型转化的趋势。     

观光木群落物种多度分布的Weibull模型研究

物种多度分布是生物多样性研究的重要内容，本文首次提出物种多度分布的Weibull分布模型．观光术群落乔木层、灌木层及乔木+灌木物种多度分布实例拟合结果表明，三者均符合Weibull分布，因此，Weibull分布模型应用于物种多度分布研究是理想的，从而丰富了物种多度分布的分布理论，同时也为观光木的管理及保护提供理论依据．     

鸡粪菌渣好氧堆肥过程中氨氧化古菌及氨氧化细菌群落的动态变化

以amo A基因为标记,通过Real-Time PCR和限制性片段长度多态性(Restriction fragment length polymorphism,RFLP)法对鸡粪菌渣好氧堆肥过程中的氨氧化古菌(Ammonia-oxidizing archaea,AOA)和氨氧化细菌(Ammonia-oxidizing bacteria,AOB)进行了丰度及群落结构的分析。结果表明,在堆制初期、好氧发酵高温期及后熟期,AOB的amo A基因丰度均占主导优势,是AOA的38~992倍。进入好氧发酵高温期,AOA amo A基因丰度下降至发酵前的0.9%,AOB下降至17.6%,后熟期AOA与AOB的amo A基因丰度与好氧发酵高温期相当。在上述3个阶段AOA与AOB各自存在一个绝对优势菌群,分别为Cluster 3和Nitrosomonas europaea,其中Cluster 3克隆子数目分别占整个克隆文库的70.73%、54.28%、72.45%,Nitrosomonas europaea克隆子数目分别占整个克隆文库的78.44%、93.20%、94.27%。堆肥3个阶段AOA的多样性指数变化不大,Shannon-Wiener值维持在1.53~1.60,但群落结构发生明显演替,随着堆肥温度升高,堆肥前期的一些菌群(Cluster 4、Cluster 5、Cluster 6)逐渐消失,新的菌群Cluster 1出现并成为堆肥中后期的第二大优势菌群。AOB无论是多样性指数还是群落组成,都发生剧烈的变化。AOB在堆肥前期Shannon-Wiener指数值最大(1.47),种群数最多(6个基因簇,分别为Nitrosomonas europaea Cluster,Nitrosomonas halophila Cluster,Nitrosomonas communis Cluster,Nitrosomonas nitrosa Cluster,Nitrosospira briensis Cluster,Nitrosospira multiformis Cluster);进入高温发酵期,Shannon-Wiener下降至0.45,群落结构单一,只有Nitrosomonas europaea Cluster和Nitrosomonas halophila Cluster;进入后熟期,AOB多样性及种群数得到一定程度的回升。     

Long-term trends in the abundance of Mediterranean wetland vertebrates: From global recovery to localized declines

Biodiversity loss is unevenly distributed in space and time. Species have reached critically low population sizes in some areas, and remain abundant in others. Similarly, some species may benefit from successful conservation plans, while others still experience severe population depletions driven by negative impacts of human activities. Although several indicators have been proposed to measure the fate of biodiversity, they are generally only implemented globally so their relevance for regional assessment is still unclear. Here, we calculated the first regional trend in the Living Planet Index for the Mediterranean wetlands (Med LPI), an indicator that summarizes the fate of global biological diversity based on the temporal trends in abundance of vertebrate populations. The Med LPI was based on 1641 vertebrate populations of 311 species recorded in Mediterranean wetlands from 1970–2008, in 27 different countries. We investigated whether trends in the Med LPI differed between eastern and western Mediterranean countries, which have different socio-economic contexts. Finally, we assessed whether and how the trend in the Med LPI was robust to changes in the number and identity of species considered. We found that, at the Mediterranean scale, the Med LPI increased steeply, which could be taken at first sight, as a general recovery of wetland biodiversity in this biogeographical region. However, we found highly contrasting spatial trends within the Mediterranean region: the average trend was positive for western and negative for eastern countries. Moreover, we showed that depending on the method used to estimate the trend in Med LPI, it can be sensitive to the number and identity of the species considered. We suggest that understanding the regional discrepancies of the trend in biodiversity indicators as well as their robustness to the species represented in the index will enhance progress assessment towards global and regional conservation strategies.     

中国高等级旅游资源数量与旅游人数、旅游收入的关系研究

旅游资源是旅游业发展的基础,其数量与质量直接影响区域旅游业发展的水平与规模.但在旅游资源质量基本一致的情况下,其数量对旅游发展的影响如何的研究较少,故文中选取全国及31个省区(不包括香港、澳门与台湾)17种世界级、国家级旅游资源数量、旅游人数、旅游收入的数据,对旅游资源数量与旅游人数、旅游收入的关系进行了实证分析.结果...     

Fungal abundance and distribution as influenced by clearing and land use in a vertic soil of Argentina

The influence of native vegetation clearing and different further soil managements on fungal propagule population diversity was studied in the present work. In each of the 3 years (1998, 1999, and 2000), soil samples were collected at the depth of 0–7.5 cm from sites under native vegetation (V0); naturalized prairie, cleared in 1982 (P16); conventional tillage, cleared in 1972 (T26); and direct drilling, cleared in 1958 (D40). Fungal population size and relative abundance of fungal genera were studied by plate counts and further identification of isolates on potato dextrose agar. The undisturbed site and the other sites with increasing time elapsed since native vegetation clearing and different management history showed a distinctive distribution of fungal genera. There were significant differences (p<0.05) among the sites in the abundance of fungal genera analyzed in all the 3 years. Principal component analysis based on relative fungal genus abundance differentiated the sites with 75% variance explained by the first and second components. Diversity and abundance of isolated fungal genera were increased as density of Penicillium spp. decreased, suggesting a competitive effect of this fungal genus. The largest diversity was found in the site under no-till management. The different distribution and relative abundance of the fungal genera studied seemed to be influenced strongly by the management and the presence of surface residue in the no-tilled site.     

Correlation between earthworms and plant litter decomposition in a tropical wet forest of Puerto Rico

Earthworms are recognized to play an important role in the decomposition of organic materials. To test the use of earthworms as an indicator of plant litter decomposition, we examined the abundance and biomass of earthworms in relation to plant litter decomposition in a tropical wet forest of Puerto Rico. We collected earthworms at 0–0.1 m and 0.1–0.25 m soil depths from upland and riparian sites that represent the natural variation in soils and decomposition rates within the forest. Earthworms were hand-sorted and weighed for both fresh and dry biomass. Earthworms were dominated by the exotic endogeic species Pontoscolex corethrurus Müller; they were more abundant, and had higher biomasses in the upland than in riparian sites of the forest. Plant leaf litter decomposed faster in the upland than riparian sites. We found that earthworm abundance in the upper 0.1 m of the soil profile positively correlated with decomposition rate of plant leaf litter. Ground litter removal had no effect on the abundance or biomass of endogeic earthworms. Our data suggest that earthworms can be used to predict decomposition rates of plant litter in the tropical wet forest, and that the decomposition of aboveground plant litter has little influence on the abundance and biomass of endogeic earthworms.     

Isotopic fractionation during N2 fixation by four tropical legumes

To quantify the contribution of biological nitrogen fixation (BNF) to legume crops using the ¹⁵N natural abundance technique, it is necessary to determine the ¹⁵N abundance of the N derived from BNF—the B value. In this study, we used a technique to determine B whereby both legume and non-N₂-fixing reference plants were grown under the same conditions in two similar soils, one artificially labelled with ¹⁵N, and the other not. The proportion of N derived from BNF (%Ndfa) was determined from the plants grown in the ¹⁵N-labelled soil and it was assumed that the %Ndfa values of the legumes grown in the two soils were the same, hence the B value of the legumes could be calculated. The legumes used were velvet bean (Mucuna pruriens), sunnhemp (Crotalaria juncea), groundnut (Arachis hypogaea) and soybean (Glycine max) inoculated, or not, with different strains of rhizobium. The values of %Ndfa were all over 89%, and all the legumes grown in unlabelled soil showed negative δ¹⁵N values even though the plant-available N in this soil was found to be approximately +6.0‰. The B values for the shoot tissue (B_s) were calculated and ranged from approximately −1.4‰ for inoculated sunnhemp and groundnut to −2.4 and −4.5‰ for soybean inoculated with Bradyrhizobium japonicum strain CPAC 7 and Bradyrhizobium elkanii strain 29W, respectively. The B (B_wp) values for the whole plants including roots, nodules and the original seed N were still significantly different between the soybean plants inoculated with CPAC 7 (−1.33‰) and 29W (−2.25‰). In a parallel experiment conducted in monoxenic culture using the same soybean variety and Bradyrhizobium strains, the plants accumulated less N from BNF and the values were less negative, but still significantly different for soybean inoculated with the two different Bradyrhizobium strains. The results suggest that the technique utilized in this study to determine B with legume plants grown in soil in the open air, yields B values that are more appropriate for use under field conditions.     

中亚热带丝栗栲群落物种-个体关系的研究

对中亚热带丝栗栲群落物种 个体关系研究结果表明 ,剩余标准差 (RSE)、偏差绝对值平均值 (AAD)和相对偏差绝对值平均值 (AARD) 3个评价指标之间存在极强正秩相关 ,表明它们对物种 个体关系模型评价结果具很强一致性 ,乔木层 5种曲线拟合效果最佳为y =1.85 18x2 - 7.84 86x 14 .5 ,灌木层 5种曲线拟合效果最佳为y =12 4 84e0 .1572x;群落中乔木层和灌木层物种多度分布均遵从对数正态分布 ,表明该丝栗栲群落发展较成熟 ,稳定性和生物多样性较高     

重庆市土壤有机碳库的估算及其空间分布特征

基于重庆市第二次土壤普查的1411个土壤剖面数据，结合重庆市土壤图、土地利用现状图和行政区划图，在地理信息系统技术的支持下，对重庆市土壤有机碳密度及储量进行了估算、同时引入有机碳丰度指数这一指标，对有机碳在不同土壤、不同区域以及不同景观中的分布特征进行了分析。结果表明：重庆市20cm和100cm深度的土壤有机碳储量分别为0．27Tg和1．0Tg；20cm深度的土壤有机碳密度介于0．33～30．36kg／m^2之间．100cm深度的土壤有机碳密度介于1．27～72．69kg／m^2之间；重庆市土壤有机碳库在不同土壤、不同区域以及不同景观的分布具有高度的空间变异性，100cm深度的土壤、区域和景观有机碳丰度指数分别为0．58～1．95，0．55～1．39和0．46～1．58．与气候、植被、人类活动等因素密切相关。