试论森林的经济成熟

根据生产函数分析的理论和方法,分别以序列林价和现行木材市场销售价为基础,讨论了确定森林经济成熟龄的方法。     

蝇蛆几丁糖保健功能的评价

以化学方法制备的蝇蛆脱乙酰几丁糖,按不同剂量喂饲试验动物雄性大鼠,检测其对试验动物的降血脂作用,结果表明：蝇蛆几丁糖能显著降低大鼠血清总胆固醇（ＴＣ）和甘油三酯（ＴＧ）水平。以蝇明几丁糖不同剂量喂饲昆明小鼠,检测其对小鼠抗突变和促进小肠运动作用的影响,结果表明：蝇蛆几丁糖可明显降低致突物环磷酰胺对小鼠骨髓嗜多染红细胞的微核发生率和丝裂霉素Ｃ对小鼠睾丸染色体畸变发生率,对ＴＡ９８的抗突变试验结果为阳     

日本血吸虫中国大陆株22.6kD膜相关蛋白基因的克隆与表达

以日本血吸虫 mRNA 为模板,用 RT-PCR 法快速克隆到一大小约600bp 的 DNA 片段,DNA 序列分析证实,所扩增到的 DNA 片段中含有日本血吸虫22.6kD 膜相关蛋白(Sj-22.6(Ch))基因,将该基因重组到表达型质粒 pGEX-4T 中,表达的 GST 融合蛋白分子量约48kD,用谷胱甘肽琼脂塘凝胶亲和层析柱纯化的重组蛋白不仅纯度好,而且得率高,纯化产量可达40mg·L~(-1)培养物,免疫试验结果表明该重组蛋白具有良好的抗原性,为其在血吸虫病抗感染中的免疫作用研究创造了条件.     

技术进步对新疆水利工程经济效益增长的贡献

介绍了线性生产函数估算技术进步的方法,并运用该方法计算了技术进步对新疆水利工程经济效益增长的贡献份额。     

花茶最佳素坯原料的选择

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Expression and functional analysis of the Follistatin-like 3 (FSTL3) gene in the sheep ovary during the oestrous cycle

Follistatin-like 3 (FSTL3) is a regulator of cellular apoptosis and was previously identified via RNA-Seq to be associated with follicular development in mammalian ovaries. However, the mechanism underlying the FSTL3 regulation of oestrus in sheep remained poorly understood. In this study, the oestrogen (E2) and progesterone (P4) concentrations in blood were detected, and the expression level and functional analysis of FSTL3 in the ovary were studied during the different reproductive stage in Aohan fine wool sheep (seasonal breeding breed in China). The concentrations of E2 and P4 at the anestrus were significantly lower compared to dioestrus, proestrus and oestrus stages. Higher expression levels of FSTL3 were observed in the sheep ovary, hypothalamus, and thyroid. During different reproductive stages, higher expression levels were found during the stages of dioestrus and proestrus, while lower levels were found during the oestrus and anestrus stages. Functional analysis of FSTL3 was performed in primary granulosa cells (GCs) of sheep. The concentration of E2 increased significantly after RNAi interference of FSTL3, while the P4 level decreased. FSTL3 can decrease P4 levels, which might be involved in mediating oestrous cycle in sheep.     

不同紫花苜蓿几个抗旱指标的灰色关联分析

运用灰色关联分析法,对5项抗旱指标与5个紫花苜蓿(Medicago Sativa)品种抗旱性的相关性进行了分析。关联度分析结果表明:各指标与苜蓿品种抗旱性的关联序为:游离脯氨酸含量>SOD>POD>相对含水量>叶片水势。各指标对苜蓿抗旱性的影响,以游离脯氨酸含量最大,其次为SOD及POD;再次相对含水量;而叶片水势的影响最小。     

旅游网络地理信息系统设计与开发初步探索

网络作为新的旅游信息平台，为旅游业的发展提供了新的契机，旅游网络地理信息系统的开发就成了形势的紧迫需要。首先，介绍了旅游网络地理信息系统开发的目的、对象，设定了系统开发的软硬件开发环境。然后详细阐述了开发旅游网络地理信息系统的结构设计思路、系统流程和系统数据库设计思想，最后说明了旅游网络地理信息系统实现的六大功能。对省、市各级地方和景区旅游网络信息系统的开发提供了有益的见解。     

Inactivation of the Wnt/β-catenin signaling contributes to the epithelial barrier dysfunction induced by sodium oxalate in canine renal epithelial cells

High oxalate consumption has been recognized as a risk factor for renal calcium oxalate stones in companion animals (dogs and cats). However, the cellular signaling involved in oxalate-induced dysfunction in renal tubular epithelial cells remains not fully elucidated. In this study, Mardin–Darby canine kidney (MDCK) cells, an epithelial cell line derived from canine kidney tubule, were tested for cell proliferation activity and barrier function after being exposed to sodium oxalate (NaOx). Further, the involvement of Wnt/β-catenin in NaOx-induced renal epithelial barrier dysfunction was evaluated. MDCK cells treated with NaOx exhibited reduction in cell proliferation and migration. Besides, NaOx exposure led to a decrease in transepithelial electrical resistance and an increase in paracellular permeability. The deleterious effects of NaOx on epithelial barrier function were related to the suppressed abundance of tight junction proteins including zonula occludens, occludin, and claudin-1. Of note, protein levels of β-catenin and phosphorylated (p)-β-catenin (Ser552) in MDCK cells were repressed by NaOx, indicating inhibitory effects on Wnt/β-catenin signaling. An inhibition of glycogen synthase kinase-3β (GSK-3β) by SB216763 enhanced the abundance of β-catenin and p-β-catenin (Ser552), and protected against epithelial barrier dysfunction in NaOx-treated MDCK cells. The results revealed a critical role of Wnt/β-catenin signaling in the epithelial barrier function of MDCK cells. Activation of Wnt/β-catenin signaling might be a potential therapeutic target for the treatment of oxalate-linked renal stones.     

大修汽车可靠性分析中故障分布规律的研究

从分布函数拟合的基本原理入手,把回归分析方法和优化技术有机结合起来,提出了一种由观测数据拟合其分布函数的优化方法,并以威布尔分布为例,说明其具体过程,通过试验数据应用表明,该方法正确可行。