草鱼出血病的电镜观察

草鱼出血病是由呼肠孤病毒感染所致的急性传染病，由于病鱼小血管受损引起全身性出血，并造成内脏器官和体壁肌肉组织局灶性病变。电镜观察表明病变细胞内各种细胞器的数量较正常细胞显著减少，形态结构也遭损坏。质膜的三层结构模糊不清，及至解体消失。与机体代谢密切相关的粗面内质网、滑面内质网、线粒体和高尔基复合体的数量减少及结构受损，使蛋白质、糖类和脂质合成及分解等一系列细胞代谢活动不能正常进行，加速了病鱼死亡。     

小型彩色扫描声纳的显示系统

近年来我省先后引进了数台WESMAR（威士玛)小型渔用扫描声纳，经试用有一定效果。这些声纳的最大特点是体积小、结构简单、显示图象直观、清晰，监能以不同颜色将目标的不同反射强度清晰地表示出来，无疑对探测、跟踪渔群带来了方便。本文根据这几年参加安装、维修工作搜集的一些资料，结合自己的体会就声纳的基本组成及其显示部份作一简单介绍。     

养殖欧洲鳗鲡狂游病病理变化的电镜观察

养殖欧洲鳗鲡狂游病病理变化的电镜观察ＥＬＥＣＴＲＯＮＭＩＣＲＯＳＣＯＰＩＣＯＢＳＥＲＶＡＴＩＯＮＯＮＰＡＴＨＯＬＯＧＩＣＣＨＡＮＧＥＳＯＦＩＲＲＩＴＡＢＬＥＳＷＩＭＤＩＳＥＡＳＥＡＳＳＯＣＩＡＴＥＤＷＩＴＨＣＵＬＴＵＲＥＤＥＵＲＯＰＥＮＥＥＬ樊海平徐...     

Effects of Various Degrees of Antennal Ablation on Mating and Oviposition Preferences of the Diamondback Moth,Plutella xylostella L.

Using scanning electron microscopy, we investigated the distribution of the trichoid, basiconic, and coeloconic sensilla on the antennae of the diamondback moth （DBM;Plutella xylostella）. The trichoid sensilla were the most abundant sensory organ, and the male moth antennae host signiifcantly more trichoid sensilla than female moth antennae. Conversely, basiconic and coeloconic sensilla were found more frequently on female than on male antennae. We performed experiments with various degrees of antennal ablation and demonstrated that DBM antennae played a key role in the control of mating and oviposition. We found that neither oviposition preference nor mating behaviors changed signiifcantly when less than 1/4 of both antennae were removed. However, there was a signiifcant behavioral change when the antennae were ablated by more than half. As the length of the antenna was shortened, the successful mating rate decreased and mating peak was delayed. An otherwise consistent host preference for oviposition was eliminated when both antennae were completely removed. Furthermore, we found that the number of trichoid sensilla was positively correlated with mating rate and oviposition preference. However, the numbers of basiconic and coeloconic sensilla were not correlated with mating rate and mating peak, but highly correlated with oviposition preference. Taken together, our results indicate that antennal sensory information plays a critical role in the mating and oviposition behaviors of this economically important pest.     

薄层扫描法测定栀子中栀子苷的含量

[目的]建立利用薄层扫描法测定栀子中栀子苷含量的方法。[方法]样品经超声提取,采用硅胶G高效薄层板条带点样,以氯仿-乙酸乙酯-无水甲醇（3：2：1,V／V／V）为展开剂上行展开,在240nm波长进行扫描检测。[结果]在0．240—0．640μg/ml范围内,栀子苷浓度与峰面积呈良好线性关系,相关系数r=0．99954,平均回收率为100．45％,RSD为1．69％。[结论]该方法简便、准确、重复性好,可用于赣南野生黄栀子中栀子苷的含量测定。     

ERIC-PCR指纹图谱及电镜技术在纳豆生产菌鉴定中的应用

纳豆是一种传统的日本食品,由纳豆菌在一定温度湿度条件下发酵大豆而来,新鲜纳豆表面金黄色,风味独特营养丰富。纳豆的主要发酵菌为纳豆菌,属芽孢杆菌属,称为纳豆枯草芽孢杆菌(Bacillus subtilis var.natto),是好氧的革兰氏阳性菌。利用扫描电子显微镜和ERIC-PCR指纹图谱技术对4株纳豆菌(高桥纳豆、MIZKAM 1、寿纳豆、MIZKAM无臭)进行了亲缘关系鉴定。结果显示,MIZKAM无臭与寿纳豆的亲缘关系较近,而高桥纳豆与其他品种亲缘关系较远。     

基于慧鱼模型的智能草坪修剪机设计

利用慧鱼创意组合模型,通过装置设计、软件编程和模块组装等环节,设计制作了智能草坪修剪机.智能草坪修剪机可根据草坪的大小以及实际形状,实现对草坪的自动化修剪.本文介绍了该装置主要元件和功能,重点分析了修剪轨迹扫描机构,通过程序控制可改变其运动路线,实现避开障碍物等功能.     

基于ARCGIS的林业基本图矢量化研究

扫描矢量化是获得矢量数据的重要方法.ArcScan结合ERDAS的栅格图像处理功能和ARCGIS的空间数据编辑、管理和分析功能,组成了一个完整林业基本图的矢量化解决方案,总结分析了ArcScan矢量化软件模块的特点和操作流程,具有效率高、数据质量高等特点,满足当前林业管理和生产活动需要.     

牛血液多形核白细胞吞噬功能检测方法的研究

以啤酒糖酵母（Ｓａｃｃｈａｒｏｍｙｃｅｓｃｅｒｅｖｉｓｉａｅ）为吞噬材料，用吖啶橙染色指示牛血液多形核白细胞的吞噬活性。该方法直观、简便、可靠，一项试验可同时获得吞噬百分率、杀伤百分率、吞噬指数和杀伤指数４项指标，从不同角度反映了机体免疫功能状态。此外，用透射电镜和扫描电镜观察了牛血液多形核白细胞吞噬经牛血清调理的啤酒糖酵母的情况。     

浅析三维激光扫描系统的自动化技术

在传统视觉测量系统的应用过程中,其测量精度通常会受到环境光背景噪声、表面散斑等因素的影响,标定方法的操作相对较为复杂、繁琐,而三维激光扫描系统有很大不同,笔者通过分析了三维激光扫描系统的标定自动化技术,之后研究了三维激光扫描系统的精度。