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ZHANG Qiong WAN Chang-wu YU Yan-ni XIA-Bing LIU Jiang-jin ZHANG Qiao-jun LI Zhu WANG Cheng-fei DAI Jia-lin WANG Jie 《园艺学报》2021,36(12):2133-2138
AIM To explore the possible mechanism of cathepsin C (CTSC) and tumor necrosis factor-α (TNF-α) in coronary heart disease (CHD) by detecting the protein expression of CTSC and TNF-α in human coronary artery tissue. METHODS The coronary artery tissues from 52 cases of CHD and 25 cases of accidental death without any heart disease in the Forensic Judicial Expertise Center of Guizhou Medical University from October 2018 to December 2019 were collected as CHD group and control group, respectively. The coronary artery stenosis and intimal plaque formation were examined by histopathology, the protein expression of CTSC and TNF-α was determined by Western blot, and the intracellular expression of CTSC and TNF-α was analyzed by immunohistochemical staining. RESULTS The results of HE staining showed that the intima of coronary artery in control group was smooth, and no thickening or stenosis was observed. In CHD group, the intima thickened irregularly, atherosclerotic plaques formed, the intima became thinner and the lumen showed eccentric stenosis in varying degrees (P <0.05). The results of Western blot showed that the expression of CTSC and TNF-α in CHD group was significantly higher than that in control group (P <0.05). Immunohistochemical staining showed that both CTSC and TNF-α were expressed in the cytoplasm of foam cells, and their positive expression was significantly higher than that in control group (P <0.05). The results of Pearson moment correlation analysis showed that there was positive correlation between the expression of CTSC and TNF-α in CHD (r2 =0.743, P <0.05). CONCLUSION The up-regulated expression of CTSC in coronary artery tissue may promote the expression of TNF-α and affect the occurrence and development of CHD. 相似文献
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《The Journal of Applied Poultry Research》2006,15(1):48-57
A study was conducted with male chicks of a commercial broiler strain to evaluate the effects of different dosage levels of a commercial α-galactosidase enzyme. Diets were formulated based on corn and soybean meal to meet the nutrient standards of top poultry companies. The positive control diet was formulated with no adjustment in the ME content of the soybean meal associated with enzyme supplementation. The negative control diet was formulated assuming a 10% improvement in the ME of the soybean meal. The negative control diet was supplemented with 0, 1.5, 3.0, 4.5, or 6.0 g of enzyme per kilogram of soybean meal to provide for 0, 45, 90, 135, or 180 galactosidase units (GALU)/kg of soybean meal. Each of the test diets was fed in mash form to 8 replicate pens of 30 birds. Body weight, feed efficiency, mortality, and calorie conversion were determined at 14, 35, and 42 d of age. Body weight, feed efficiency, and mortality were not significantly (P < 0.05) affected by dietary treatment. Birds fed the negative control with no enzyme supplement were numerically reduced in body weight or feed efficiency as compared with the control diet; however, addition of the enzyme was without benefit. When unadjusted energy values were analyzed, birds fed the negative control diet were more efficient in calorie conversion, but this finding was unrelated to enzyme addition, suggesting that diet composition was responsible for the differences in calorie utilization. When adjusted energy values were compared, birds fed the negative control tended to have higher (less efficient) calorie conversion than those fed the positive control with little or no indication of improvement from the addition of the α-galactosidase enzyme. Results of this study show no benefit from the addition of the enzyme used in this trial. 相似文献
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乙酰水杨酸调控猕猴桃果实后熟软化进程中的Ad-EXP1基因表达 总被引:1,自引:0,他引:1
以布鲁诺猕猴桃(Actinidia deliciosa cv.Bruno)成熟果实为材料,根据植物α-expansin(EXP)基因的氨基酸保守序列设计简并引物,利用RT-PCR方法结合3’RACE扩增得到1个长为957bp的cDNA片段(Ad-EXP1)。该基因片段编码211个氨基酸,与其它植物该基因核苷酸同源性为59%~85%,氨基酸同源性为73%~91%。Northern杂交结果表明,Ad-EXP1基因在采收当天的果实中表达很弱,随着果实成熟衰老进程加快趋于增强;乙酰水杨酸(ASA)处理延缓果实后熟软化和乙烯生成,也显著抑制Ad-EXP1基因表达。鉴于Ad-EXP1表达丰度与乙烯生成、果实软化程度的一致性,推测该基因在猕猴桃果实后熟软化进程中起着重要作用。 相似文献
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家蚕微孢子虫(浙江株)α-微管蛋白基因部分片段的克隆及系统发育分析 总被引:1,自引:4,他引:1
基于微孢子虫分类学地位的争议,通过克隆家蚕微孢子虫(Nosema bombycis)的α-Tubulin基因,利用BioEditor将核苷酸序列翻译成氨基酸序列,并从NCBI中收集不同物种的α-Tubulin基因,用CLUSTALX(1.81)、Mega2以及在线生物信息学软件CLUSTALW(1.83)分析序列并构建系统发育树。结果显示:家蚕微孢子虫及其它微孢子虫与真菌聚为一类,且微孢子虫以一个独立群与接合菌(zygomycetes)的噬虫霉属(Entomophaga)、耳霉属(Conidiobolus)关系最近,与子囊菌(ascomycetes)、担子菌(basidiomycetes)、壶菌(chytrids)及其它接合菌互为姐妹群。 相似文献
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为探究热加工方式对牛乳过敏原αs1-酪蛋白构象和抗原性的影响,利用间接竞争酶联免疫吸附测定方法、
免疫印迹方法分析不同加热条件下αs1-酪蛋白免疫原性的变化,进而通过8-苯胺-1-萘磺酸荧光探针和圆二色谱分
析其二级结构变化,初步揭示热处理调控过敏原αs1-酪蛋白抗原性的机制。结果表明:在80 ℃、60 min,90 ℃、
10 min,90 ℃、60 min条件下热处理后,αs1-酪蛋白中α-螺旋结构含量显著低于未加热αs1-酪蛋白,在70 ℃、
20 min,80 ℃、20 min,90 ℃、20 min条件下热处理后,αs1-酪蛋白中无规卷曲含量显著增加,70~100 ℃加热
20 min条件下表面荧光强度最强,其他温度-时间条件下二级结构含量变化不显著;αs1-酪蛋白构象的变化导致αs1-酪
蛋白的抗原性显著降低,间接竞争酶联免疫吸附测定显示,在70~100 ℃加热20 min条件下,αs1-酪蛋白的抗原残留
量均较高,而免疫印迹方法显示不同温度-时间条件下αs1-酪蛋白仍具有免疫反应特性,建议进一步通过动物实验揭
示热处理调控αs1-酪蛋白抗原性的机制。 相似文献
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