水资源污染负荷强度的灰色有效性预测模型及其应用

水资源污染负荷强度预测是水污染防治的关键环节。基于灰色系统理论,构建了水资源污染负荷强度的GM(1,1)模型、Verhulst模型和SCGM(1,1)c模型,并利用预测有效度计算各单预测模型的权重,进而建立水资源污染负荷强度的灰色GM-Verhulst-SCGM组合预测模型,在此基础上,选取2004-2013年期间工业单位产值化学需氧量排放量历史数据进行模型拟合,利用其2014-2016年数据进行模型检验。研究发现,灰色组合预测模型呈现出更低的预测误差,符合水资源污染负荷强度高精度预测需求;而通过对水资源污染负荷强度实证预测发现,其负荷强度整体上呈逐步削弱的态势,但可能会出现其高速下降向稳步趋缓转变的速率"拐点",预示着水污染防治将由"浅水区"向"深水区"的转变。     

我国玉米期现货市场的风险传递效应研究

以2016～2017年我国玉米期现货市场为基础,采用双变量EC-EGARCH模型对我国玉米期货市场和现货市场之间的价格引导关系及风险传递效应进行研究。结果表明,我国玉米期货与现货价格之间具有长期均衡关系,期货价格对市场信息的反应速度较快,对现货市场具有引导作用。将协整残差项作为解释变量引入均值方程和条件方差方程,对期货价格与现货价格均有明显的调节作用,更加准确地刻画了二者之间的关系,且协整残差项对期货市场的波动具有负向影响,对现货市场的影响不显著。期货对现货具有单向波动溢出效应,印证了玉米期货对现货市场的引导作用。     

菌根真菌对3种兰花幼苗生长作用研究

菌根真菌在兰科植物生活史中具有重要作用。为获得促进兰科植物生长的共生真菌,阐明菌根真菌对兰科植物的作用,本研究对从野生建兰中分离获得的3株共生真菌进行形态学和系统进化分析,并将3株真菌与建兰、硬叶兰和铁皮石斛幼苗在不同浓度燕麦琼脂培养基(OMA)条件下共培养,进行比较研究。结果表明,3株真菌分属于胶膜菌属(Tsc)、角担菌属(Cej3)和蜡壳菌属(Sec),且菌丝和菌落特征存在明显差异;系统进化分析发现,Tsc菌株与Cej3菌株亲缘关系较近,与Sec菌株亲缘关系较远;与兰科植物共培养结果表明,Tsc菌株可在全部OMA浓度条件下与3种兰科植物幼苗建立共生关系,且定殖率均为100%;Cej3菌株和Sec菌株与3种兰科植物在不同OMA浓度条件下定殖时间及定殖率存在差异,且具有一定的专一性;与对照组相比,与真菌共生的兰科植物幼苗的平均鲜重、干重、苗高、根长增长率均明显提高。本研究结果为深入研究兰科植物与真菌共生的分子机制奠定了一定的理论基础,并为兰花种质资源的保护提供了参考。     

小流域非点源污染模拟与生态修复影响评价

[目的]基于各流域场次降雨径流过程和非点源污染负荷模拟分析,评价小流域生态建设对密云水库流域水文过程和污染防控效果影响,为水源地保护、流域山水林田湖一体化修复和生态环境建设提供科学依据。[方法]以北京市重要饮用水源地密云水库上游蛇鱼川小流域为研究对象,利用EcoHAT-LCM模型和传统大尺度非点源污染模型相结合的模式进行模拟分析,并设置情景,分析评价小流域生态建设效果。[结果]随着场次雨量的增加,吸附态N,P负荷占污染负荷总量的比重在逐渐增加,溶解态N,P负荷占污染负荷总量的比重在逐渐减少。小流域内居民点具有小城镇级别的垃圾处理率和垃圾入网率时,农村生活N,P污染负荷可以削减28%;减少经济林的现有施肥量,农业生产活动的N,P污染负荷能够削减43.9%和48.9%。[结论]研究区域不同频次暴雨对吸附态N,P负荷的影响大于对溶解态N,P负荷的影响。生态修复措施对N,P污染负荷削减作用显著。     

Historical bank-breachings of the lower Yellow River as influenced by drainage basin factors

The Yellow River is famous for sediment-related disasters in history. The bank-breaching frequency of the lower Yellow River has been adopted as a proxy index to study the historical sediment-related disasters, and then it has been related to various indices describing the natural and human factors of the Yellow River basin. Consequently, some correlations have been established.Climate has profound influences on the bank-breaching frequency of the lower Yellow River. Before the Sui and Tang Dynasties (681–907 AD), natural vegetation in the middle Yellow basin was not yet significantly affected by man. With the increase in the humid index, growth of vegetation became greater and the bank-breaching frequency of lower Yellow River declined. After the 8th Century, however, the relation between bank-breaching frequency and the humid index became inverse, i.e., with the increase in the humid index, bank-breaching frequency also increased. This is because, to a great degree, the natural vegetation had been destroyed since the 10th Century.The bank-breaching frequency can be positively correlated to the frequencies of both large flood and drought disasters. The positive correlation between bank-breaching frequency and frequency of large droughts is because the Yellow River channel decreased in size during dry periods; when large floods came, bank-breaching was more likely to occur.Human activities are the major factor influencing sediment-related disasters in the lower Yellow River. Historically, the increase in population led to a higher bank-breaching frequency, and the periods of low population corresponded to the periods with low frequency of bank-breaching. The increase in the bank-breaching frequency of the lower Yellow River occurred in association with the northward shift of the agriculture–animal husbandry transitional zone, and when the agriculture–animal husbandry transitional zone shifted southward, the bank-breaching frequency declined.     

Estimation of irrigation requirements for drip-irrigated maize in a sub-humid climate

Drip-irrigation is increasingly applied in maize (Zea mays L.) production in sub-humid region. It is critical to quantify irrigation requirements during different growth stages under diverse climatic conditions. In this study, the Hybrid-Maize model was calibrated and applied in a sub-humid Heilongjiang Province in Northeast China to estimate irrigation requirements for drip-irrigated maize during different crop physiological development stages and under diverse agro-climatic conditions. Using dimensionless scales, the whole growing season of maize was divided into diverse development stages from planting to maturity. Drip-irrigation dates and irrigation amounts in each irrigation event were simulated and summarized in 30-year simulation from 1981 to 2010. The maize harvest area of Heilongjiang Province was divided into 10 agro-climatic zones based on growing degree days, arid index, and temperature seasonality. The simulated results indicated that seasonal irrigation requirements and water stress during different growth stages were highly related to initial soil water content and distribution of seasonal precipitation. In the experimental site, the average irrigation amounts and times ranged from 48 to 150 mm with initial soil water content decreasing from 100 to 20% of the maximum soil available water. Additionally, the earliest drip-irrigation event might occur during 3- to 8-leaf stage. The water stress could occur at any growth stages of maize, even in wet years with abundant total seasonal rainfall but poor distribution. And over 50% of grain yield loss could be caused by extended water stress during the kernel setting window and grain filling period. It is estimated that more than 94% of the maize harvested area in Heilongjiang Province needs to be irrigated although the yield increase varied (0 to 109%) in diverse agro-climatic zones. Consequently, at least 14% of more maize production could be achieved through drip-irrigation systems in Heilongjiang Province compared to rainfed conditions.     

Validation of an extraction method for the quantification of soluble free and insoluble bound phenolic compounds in wheat by HPLC-DAD

Wheat is one of the three most widely grown grain crops and the most consumed in Europe. Wheat contains different phenolic compounds, mainly phenolic acids and flavonoids that are not evenly distributed in the wheat, being most abundant in the bran. These phenolic compounds are antioxidants against free radicals and other reactive oxygen species. Therefore, over the last few decades, there is a great interest to study and quantify these compounds present in wheat.This work proposes a HPLC-DAD method that allows a good separation of different phenolic compounds occurring as soluble free and insoluble bound compounds in wheat brand and flour. The extraction and the chromatographic conditions allowed the characterization of forty-two phenolic compounds, among phenolic acids (HBA, HCA, DFA and TFA), phenolic aldehydes, phenolic alcohols and flavonoids (flavones). This method is simple, sensitive, reproducible and accurate, and only a HPLC-DAD equipment is required. Thirty-four soluble and insoluble phenolic compounds can be quantified in wheat flour and brand. Hydroxytyrosol, a potent antioxidant compound, has been identified and quantified in wheat for the first time.     

昕水河流域径流变化及其对气候和人类活动的响应

开展流域尺度气候变化和人类活动对径流的影响研究是科学制定流域综合管理规划,并实现流域可持续管理的基础。以黄土高原丘陵沟壑区的典型流域——昕水河流域为研究对象,结合流域多年(1958—2015年)气象水文数据,采用M-K趋势检验法分析年降水、年潜在蒸发散和年径流量的变化趋势,运用双累积曲线法分析年径流量序列的突变年份,并根据生态水文分析法与水量平衡原理定量解析流域尺度气候变化和人类活动对年径流的影响。结果表明:1958-2015年,流域年径流量变化呈显著下降趋势(Z=-5.84,p<0.0001),而降水(Z=-0.72,p=0.31)和潜在蒸散发(Z=-0.5,p=0.88)变化趋势不显著。双累积曲线法表明径流量突变点为1974年和2000年,其中,1975—2000年间影响径流减少的主要因素是气候变化,气候变化导致径流量减少的贡献率为73.14%,土地利用方式仅为26.86%;而2001—2015年土地利用变化是影响径流减少的主要原因,其贡献率高达103.81%,可见土地利用变化已成为昕水河流域径流量变化的主要驱动因素。在今后的流域治理中,需合理制定土地利用方案,实现昕水河流域水土资源协调发展。     

内蒙古甜菜制糖产业发展探析

内蒙古自治区从1955年包头糖厂建成投产开始,已成为我国重要的甜菜制糖产业基地。近年来内蒙古甜菜制糖产业快速发展,甜菜制糖企业呈现集团化、多元化、专业化的格局,共有13家糖厂在生产,日加工甜菜能力达5.35万吨,食糖年产能70万吨。内蒙古具有较强的甜菜生产技术优势,甜菜种植的比较收益突出,通过甜菜制糖企业合理布局,建立甜菜种植保护区,依靠科学技术,规模化种植提高了甜菜生产机械化水平,合理轮作,避免甜菜重迎茬,建立环境友好型甜菜生产方式,形成稳定的甜菜生产能力,为制糖企业提供充足原料。采用先进的制糖工艺技术,降低糖分工艺损失,加快新产品开发,延伸甜菜制糖产业链,以效益为中心,推动甜菜制糖行业可持续发展。2018/2019榨季内蒙古食糖产量达到历史高位的65万吨,占国内甜菜糖总产量的49.04%,成为我国最大的甜菜糖产区。     

Effect of decitabine on resistance of K562/A02 cells to adriamycin

AIM: To investigate the effect of decitabine (DAC) on the resistance of human chronic myeloid leukemia cell line K562/A02 to adriamycin (ADR), and to explore the possible mechanism. METHODS: The K562/A02 cell line and its parental cell line K562 were treated with different concentrations of ADR or DAC alone, or in combination. The cytotoxic effects of these 2 agents were determined by CCK-8 assay. The degree of DNA methylation was evaluated by Sequenom MassARRAY system and colorimetric method. The cell cycle distribution and the apoptotic rate were determined by flow cytometry. RESULTS: K562/A02 cells were more significantly resistant to ADR than K562 cells.The half maximal inhibitory concentration of ADR for 24 h of the K562/A02 cells was about 50 times higher than that of the K562 cells. To DAC, in the concentration range of 0.5~8 μmol/L, K562/A02 cells were more sensitive than K562 cells. As compared with the same concentrations (4.31 μmol/L and 17.24 μmol/L) of ADR alone, the combination with 1 μmol/L DAC significantly improved the sensitivity of K562/A02 cells to ADR. Both DAC and ADR affected the cell cycle progression and apoptotic rate of K562/A02 cells. DAC (1 μmol/L) treatment mainly showed S phase arrest and increased early apoptotic rate for 24 h, and G₂/M phase arrest and increased late apoptosis and necrosis for 48 h in a time-related manner. ADR treatment showed G₂/M phase arrest and increased late apoptosis and necrosis in a concentration-dependent manner. In combination with 1 μmol/L DAC, the effect of ADR on the cell cycle distribution was further enhanced, showing more obvious G₂/M phase arrest, but no significant difference of the apoptotic rate was observed. The degree of methylation in the genome had no significant difference between the 2 cells, and it before and after DAC treatment had no significant change. CONCLUSION: DAC enhances the sensitivity of K562/A02 cells to ADR, showing drug resistance-reversing potential. The mechanism may be related to regulating cell cycle progression and promoting apoptosis and necrosis of K562/A02 cells.