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ZHONG Xing-fu WANG Pei-kun YANG Yong-li QIN Li-li BI Yu-yu ZOU Guang-zhen PENG Hao WEI Ping 《中国畜牧兽医》2015,42(7):1647-1653
In order to investigate the infection status of avian leukosis virus (ALV) and avian reticuloendotheliosis virus (REV) in the major local breeds of Qinzhou,Guangxi,totally 953 samples of egg white,cloaca swab and serum of Ma duck,Shitou goose,Tiejiao-Ma chicken,turkey and pigeon were collected from the representing flocks and detected by the commercial ELISA kits.ALV was isolated for the ALV p27 positive samples by culturing on DF-1 cells,and gp85 gene was sequenced.The results showed that the detections of ALV were negative in the samples except those of Tiejiao-Ma chicken,while REV antibody was found positive in Ma duck,Tiejiao-Ma chicken and turkey.The nucleotide sequences of gp85 gene of two isolates shared 94.5% identity with each other,and shared 86.9% to 94.9% with reference strains.The amino acid sequences of gp85 gene of two isolates shared 91.5% identity with each other,and shared 84.0% to 91.6% with reference strains.There were many variable sites in the hyper variable region hr1 and hr2,and the vr2 and vr3 variable regions were relatively conservative.Phylogenetic tree analysis showed that the two isolates shared the highest homology with SCAU11-XG strain. 相似文献
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研究了不同阴香群落中幼苗营养元素的含量及其与土壤养分之间的关系。结果表明:阴香幼苗营养元素含量幅度分别为:N 15.3-4.1 g/kg;P 0.4-0.2 g/kg;K 4.9-2.3 g/kg;Ca 16.1-1.9 g/kg。阴香幼苗元素含量差异显著,各器官中以叶的营养元素含量最高。各元素中以Ca的变异程度最大,变异系数为61.12%,K的变异程度最低,变异系数为25.95%。阴香幼苗各元素之间相关性显著,但与土壤养分之间相关性不显著,这主要是由阴香的生物生态学特性所决定的。 相似文献
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稻草持续还田定位监测结果与分析 总被引:3,自引:0,他引:3
监测结果表明,稻草持续还田能提升土壤有机质、增进氮磷钾养分含量,改善土壤结构,提高土壤保水保肥性能,增强土壤微生物活性,优化农田生态环境,提高土壤综合生产能力,对推进耕地质量建设,促进农业可持续发展具有重要的意义。 相似文献
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在砂页岩母质发育的酸性赤红壤上种植甘蔗新台糖25号,进行测土配方施肥试验研究。结果表明:测土配方施肥对提高甘蔗产量、糖分含量具有明显的促进作用,与常规施肥和不施肥相比,产量分别增加6.53t/hm2和40.24t/hm2,增长7.5%和75.2%;糖分含量分别提高0.8%和1.8%;产糖量分别增加1629.2kg/hm2和6919.6kg/hm2,增长12.5%和89.8%;生产成本降低454元/hm2,净增效益分别增加2935元/hm2和8813元/hm2,增长11.2%;和43.3%,增产增收效果显著。 相似文献
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