通过病毒分离鉴定和基因检测首次发现虎流感

应用F81猫肾传代细胞，从高热、拒食和间有神经症状的死亡率病科中分离获得1株病毒，经形态学、理化学、生物学和血清学系统鉴定，证明为流感病毒。采用流感病毒核蛋白基因引物，对分离病毒及该虎病科进行RT－PCR扩增和序列分析，结果从分离病毒和虎病科中均扩增出与理论值大小相符的464bp基因片段；其序列与A、B、C3型流感病毒相比较，同源性分别为84．9％、35．0％、24．8％。由此说明，所分离病毒为A型流感病毒，命名为/蘧／哈尔滨（中国）／01／2002。用此分离病毒静脉接种于3月龄家猫3号，均出现与病虎相似的临床症状，其中1号耐过，2只死亡。死亡猫剖检变化与死虎相似，主要是呈肺炎病变，并可从病科中回收到所接种病毒。从此分离病毒为HA抗原，进行虎与猫血清的HI抗体检测，结果康复虎血清比其病初血清、康复猫血清比其接种前血清HI抗体均增高4倍以上，表明该病毒具有致病性，是引起该虎与试验猫发病甚至死亡的病原。     

扫描及负染电镜在兔脑炎原虫病诊断中的应用

12只临床表现头颈歪斜、昏睡、震颤、局部或全身轻瘫的可疑为兔脑炎原虫病的獭兔 ,对其肾脏皮髓交界和尿沉渣分别进行了扫描电镜和负染电镜观察 ,看到了大小为 1～ 1 .5× 1 .4～ 2 .5μm,形态呈卵圆形和杆状的兔脑炎原虫。阳性检出率为 1 0 0 %。说明扫描及负染电镜术是诊断兔脑炎原虫病的一种较为可靠的技术。     

河南省杨树材种出材率表的研编

用削度方程和单株带皮—去皮胸径的转换关系式两个模型对河南省杨树进行计算机理论造材．经精度检验，满足要求，方法可行，依此编制了河南省杨树材种出材率表。     

猪瘟病毒E0基因在CHO细胞中的表达

将EGFPE0融合基因插入哺乳动物细胞表达载体pCI-dhfr中,构建重组质粒pCI-EGFPE0。将重组质粒转染CHO(dhfr-),在荧光显微镜下观察到少数细胞呈现绿色荧光,表明转染成功。通过MTX加压筛选后,在荧光显微镜下观察到多数细胞呈现强绿色荧光,表明重组融合蛋白EGFPE0在CHO细胞中得到大量表达。高效表达重组融合蛋白细胞克隆的获得为进一步大量制备可溶性的猪瘟病毒E0糖蛋白、制备其单抗筛选抗原表位及研究E0蛋白的生物学功能奠定基础。     

软籽石榴新品种‘红玉石籽’

 ‘红玉石籽’是从‘玉石籽’营养系变异中选育出的石榴新品种。果皮全面着色, 平均单果质量、百粒质量、可溶性固形物含量和出汁率分别比对照品种‘玉石籽’提高33.4%、16.8%、2.7%和3.0%。软籽, 核硬度3.31 kg/ cm²。植株抗褐斑病、干腐病, 丰产, 皖中地区9月中下旬果实成熟。     

嫁接西瓜肥料试验初报

试验结果表明,在土壤有机质含量高达4.07%的单栋钢管大棚中,进行嫁接西瓜栽培时,与常规施肥量相比,在4个减肥处理中,以减量20%的处理增产优质效果最佳。     

类鼻疽的研究进展

本文就类鼻疽(melioidosis)的流行病学,诊断方法,免疫机理,发病机理及治疗方法 进行了综述。为重视该病的研究提出了建议。     

Effects of hypoxia and hypercapnia on expression of soluble guanylate cyclase in rat pulmonary artery

AIM:To investigate the expression of soluble guanylate cyclase protein and its mRNA in rat pulmonary artery after exposure to hypoxia and hypercapnia.METHODS:Male Sprague-Dawley rats were randomly split into 4 group, which were hypoxic hypercapnic (HH 1 week, HH 2 weeks, HH 4 weeks) group and control group, to copy pulmonary hypertensive animal model. The expression of sGCα₁ and β₁ subunits protein of medial and small pulmonary artery was performed by immunohistochemistry with a polycolonal antibody. In situ hybridization was performed on the rat lung tissue using sGC oligonuclear probe to assay the expression of sGCα₁subunit mRNA.RESULTS:The sGCα₁ and β₁ subunits protein and sGCα₁ subunit mRNA were faint staining in the pulmonary small and medium artery in HH1 week, HH 2 weeks and HH 4 weeks groups compared with control group (all P＜0.01).CONCLUSION:sGC subunit mRNA and protein expression in pulmonary small and medium artery were decreased after exposure to hypoxia and hypercapnia, which took part in the development of the pulmonary hypertension.     

Study on relationship between expression of PKCα in glomeruli and development of nephropathy in diabetic rats

AIM:To observe the dynamic changes of expression of PKCα, TGF-β₁ and α-SMA in glomeruli of diabetic rats induced by the alloxon and to invesitigate their roles in the diabetic nephropathy(DN).METHODS:Rats were randomly divided into four groups: normal control group (group A), diabetic group of one week (group B), diabetic group of one month (group C), diabetic group of two months (group D). Immunohistochemistry and Western blotting were used to detect the expression of PKCα, TGF-β₁ and α-SMA in renal tissue of all groups. Blood glucose, triglycerides, cholesterol, creatinine and urine protein were analysed by chemical methods. The morphological changes of renal tissue were checked through microscopy.RESULTS:The expression of PKCα and TGF-β₁ in renal tissue of diabetic groups were increased comparing with those of nomal control group(P<0.05). The mesangial cells expressed α-SMA in two months group. Chronologically the expression of PKCα, TGF-β₁ and α-SMA were positively correlative with each other and the impairment of kidney was also observed.CONCLUSIONS: During the DN process the expression of PKCα increased. PKCα raised GFR and the permeability of glomerular filtration membrane which enhanced urinary albumin excretion. PKCα also increased expression of TGF-β and therefore to induce the expression of α-SMA. The appearance of α-SMA was a marker of the phenotypic transform of renal cells.     

转Bt基因棉在安庆市大面积种植后害虫发生特点调查

根据调查,转Bt基因棉大面积种植后,棉铃虫、红铃虫发生减轻;棉叶螨发生加重,为害时间延长;甜菜夜蛾、斜纹夜蛾发生面积扩大,为害程度加重。据此初步提出了当地转Bt基因棉害虫防治中应注意的问题和管理对策。