栀子和玉兰花提取物对烤烟主要化学成分及致香物质含量影响

以湘烟5号为材料,通过盆栽试验,探讨不同浓度植物提取物对烤烟主要化学成分及致香物质含量的影响。结果表明,喷施栀子花和玉兰花汁液提取物有利于烟叶原料主要化学成分含量的改善,与对照相比,喷施处理的上部橘黄二级（B2F）和中部橘黄三级（C3F）烟叶还原糖含量分别高15.0%~21.1%和14.3%~17.7%,钾含量分别高5.7%~13.9%和7.4%~14.0%;与对照相比,喷施处理的烟叶的石油醚提取物、类胡萝卜素降解物、降解产物、茄酮、非酶棕色化反应产物、芳香族氨基酸裂解产物、新植二烯和中性致香物质总量,上部橘黄二级（B2F）烟叶分别高10.0%~20.9%、11.2%~20.9%、9.6%~20.2%、9.8%~21.1%、10.8%~22.5%、11.2%~23.0%、14.9%~24.7%和11.9%~22.1%,中部橘黄三级（C3F）烟叶分别高13.5%~24.1%、11.3%~21.0%、11.4%~20.0%、10.8%~18.7%、13.1%~21.2%、10.1%~19.1%、17.5%~27.0%和18.1%~27.6%;烟叶感官质量评价权重得分高于对照。栀子花和玉兰花汁液提取物对烟叶主要化学成分及致香物质含量的作用效果以施用量3.5~5.0g/株的处理最佳。     

Construction and evaluation of introgression lines and fine mapping of ehd8 from Jinghong common wild rice (Oryza rufipogon)

Heading date is one of the most important traits in rice and regulated by multiple genes. Common wild rice is the ancestor of Asian cultivated rice and harbours abundant genetic diversity. To use wild rice resource in rice breeding, a set of 154 introgression lines (ILs) covering 93% of the genome of Jinghong common wild rice was constructed in the background 'Teqing', using 208 simple sequence repeat markers evenly distributed on 12 chromosomes. Among the ILs, the line JIL64 displayed late heading independent of photoperiod. Genetic analysis using the two F₂ populations crossed ''Teqing'/JIL64 and JIL64/'Teqing' revealed that late flowering was controlled by a recessive gene on chromosome 8 (designated early heading date 8, ehd8), and ehd8 was fine mapped to the 50‐kb region flanked by markers RM22221 and 64Indel4. Sequencing and qRT‐PCR demonstrated that LOC_Os08g01410 and LOC_Os08g01420 were deleted in JIL64 and may be associated with the late heading of Jinghong common wild rice. These findings lay a practical foundation for characterizing ehd8, and the ILs help to mine genes from Jinghong common wild rice.     

完善内地主产棉区棉花补贴机制的思考与建议——以山东省为例

总结了棉花目标价格补贴政策实施以来山东植棉区的实施进展、成效与问题。从国家棉花产业安全和山东省经济社会发展角度，分析了山东植棉区棉花生产的重要地位和保持补贴政策稳定性的重要意义。以山东为例，提出了内地主产棉区棉花补贴政策改革建议。     

High water content silk protein-based hydrogels with tunable elasticity fabricated via a Ru(II) mediated photochemical cross-linking method

Silk is very promising in the field of biomaterials as a natural biomacromolecule. Silk protein can be made into various forms of materials, including hydrogels. However, silk protein-based hydrogels have not attracted much attention due to its weak mechanical properties. Here, we report high water content silk protein-based hydrogels with tunable elasticity which were fabricated through Ru(II) mediated photochemically cross-linking tyrosine residues in regenerated silk protein. The regenerated silk protein was characterized by Fourier transform infrared spectroscopy (FTIR). The gelation kinetics of the silk protein was studied by rheology measurements. The compressive mechanical properties of the silk protein-based hydrogels was investigated using compressive tests and dynamic mechanical analysis (DMA). Compressive modulus of the hydrogels reached 349±64 MPa at 15 % strain. The fabricated silk protein-based hydrogels were also characterized by Scanning electron microscopy (SEM), revealing an interconnected porous network structure, typical of hydrogels, with an average pore size of approximately 130 μm. Finally, biocompatibility of the silk protein-based hydrogels was demonstrated through cell culture studies using a human fibroblast cell line, HFL1. The reported silk protein-based hydrogels represent a promising candidate for biomaterial applications.     

棉花“保险+期货”模式助力南疆主产棉区脱贫攻坚

通过解析棉花“保险+期货”模式的运行机制、优势及存在的不足，并提出了发展优质棉“订单生产+保险+期货”模式和引入银行、基金等信贷机构的建议，期望对南疆主产棉区贫困棉农增收致富、助力脱贫攻坚提供有益探索。     

浅谈楼房养猪除臭技术设计与应用

楼房养猪相较于传统平房养猪,占用面积更小,可节省用地60%以上。楼房养猪同时方便集中管理,易于采光通风和保暖;但是楼房养猪也有明显的缺点,相较于传统的平房养猪,楼房养猪作为集约化、规模化、现代化养猪的集中表现模式,建造及运营维护成本相对较高,消毒及防疫工作较为困难。高楼环境易于通风的同时,猪舍的污染问题也更加突出。猪舍给空气带来的污染不仅会影响猪舍内外的空气质量,同时还会影响猪只的繁殖、生长和发育,并将会对养殖人员与附近居民的身体健康产生重要影响。所以,必须采取相应完善的系统科学措施,处理楼房养猪中的空气除臭问题,保证生猪生活环境的清洁。文章通过结合平房养猪以及楼房养猪的特点,浅谈一下楼房养猪的除臭技术的设计与应用。     

体细胞克隆荷斯坦奶牛出生后死亡犊牛内脏器官的组织病理学观察

为探究体细胞克隆荷斯坦奶牛出生后死亡的原因,对新生后死亡的克隆荷斯坦公牛和自然繁殖的荷斯坦公犊的主要组织器官进行比较。通过解剖和石蜡切片-HE(hematoxylin-eosin staining)染色技术,对主要的组织器官结构进行观察和分析。结果表明,新生后死亡的克隆荷斯坦公牛肺部结构清晰;肝脏的肝细胞明显肿大,出现脂肪轻度变性;肾小管上皮细胞出现变性;心肌的肌纤维间空隙增大;骨骼肌纤维间隙明显,空泡变性,这可能导致该克隆牛犊肌肉无力并功能不全;淋巴结皮髓质界限不分明,淋巴小结细胞较稀疏,生发中心不明显,淋巴窦细胞较少;脾脏内红细胞较少,这说明该克隆牛的造血功能可能不完善;胸腺的皮质部分和髓质部分界限不明显,嗜酸性胸腺小体不易辨认,可能发育不全。该克隆公牛免疫器官出现不同程度的发育不全现象较严重,这有可能是其出生后死亡率高的主要原因。     

羊支原体性肺炎两标准株对抗菌药物敏感性的研究

本实验测定了环丙沙星、氧氟沙星、单诺沙星、红霉素、罗红霉素、泰勒菌素、泰妙菌素、四环素等8种药物对羊肺炎支原体两个标准株Y98和Y-goat的体外抑菌浓度以及红霉素与氧氟沙星、泰勒菌素对Y-goat和四环素与氧氟沙星、泰勒菌素对Y98的联合药敏作用。对羊肺炎支原体两个标准株对抗菌药物的敏感性进行了分析。     

预应力薄腹梁质量事故的分析及鉴定

阐述了某工程由于施工原因，导致预应力混凝土工字形屋面梁强度不足的预制构件的检验鉴定过程和方法。采用回弹法推定混凝土实际强度；通过静载试验测试构件的实际挠度、抗裂和承载能力。分析了导致混凝土强度不足的原因。     

甜菜坏死黄脉病毒(BNYVV)75kDa通读蛋白基因及其54kDa片段的克隆与表达

 以甜菜坏死黄脉病毒(BNYVV)内蒙分离物的总RNA为模板,通过反转录和PCR扩增获得75kDa通读蛋白基因54kDa片段的目的片段。