体细胞克隆荷斯坦奶牛出生后死亡犊牛内脏器官的组织病理学观察

为探究体细胞克隆荷斯坦奶牛出生后死亡的原因,对新生后死亡的克隆荷斯坦公牛和自然繁殖的荷斯坦公犊的主要组织器官进行比较。通过解剖和石蜡切片-HE(hematoxylin-eosin staining)染色技术,对主要的组织器官结构进行观察和分析。结果表明,新生后死亡的克隆荷斯坦公牛肺部结构清晰;肝脏的肝细胞明显肿大,出现脂肪轻度变性;肾小管上皮细胞出现变性;心肌的肌纤维间空隙增大;骨骼肌纤维间隙明显,空泡变性,这可能导致该克隆牛犊肌肉无力并功能不全;淋巴结皮髓质界限不分明,淋巴小结细胞较稀疏,生发中心不明显,淋巴窦细胞较少;脾脏内红细胞较少,这说明该克隆牛的造血功能可能不完善;胸腺的皮质部分和髓质部分界限不明显,嗜酸性胸腺小体不易辨认,可能发育不全。该克隆公牛免疫器官出现不同程度的发育不全现象较严重,这有可能是其出生后死亡率高的主要原因。

The Internal Organs of Dead Calf of Somatic Cloning Holstein Cows: Histopathological Observation

To investigate the death causes of cloned Holstein bulls, we compared major tissues and organs of the neonatal-death cloned holstein bull and natural propagation Holstein bull calf. We observed and analyzed the main tissues and organs by anatomy and HE (hematoxylin-eosin staining) staining. The results showed that the lung structure of the neonatal-death cloned Holstein bull was clear. Its liver cells were obviously swollen and the liver had slight degeneration. The renal tubular epithelial cells of the kidney had slight degeneration. The interstitial space between the myocardium fibers was enlarged. Skeletal muscle fiber gap was obvious, and vacuolar degeneration, which might lead to muscle weakness and insufficiency of the cloned bull. The boundaries of the lymphoid cortex and medulla were not distinct. Lymph node cells were sparse, germinal center was not obvious, and lymphatic sinus cells were less. There were fewer red blood cells in the spleen, which might indicate that the cloned bull had imperfect hematopoietic function. The boundary between the cortical and the medulla of the thymus was not obvious. The eosinophilic thymus gland was not easily identifiable and might be hypoplasia. The cloned Holstein bull’s immune organs had different degrees of hypoplasia, which might be the main reason for the high mortality after birth.