Effects of dietary Bacillus coagulans and yeast hydrolysate supplementation on growth performance,immune response and intestinal barrier function in weaned piglets

The present study investigated the effects of Bacillus coagulans and yeast hydrolysate supplementation on growth performance, immune response and intestinal barrier function of weaned piglets. Twenty-four weaned piglets with an average body weight (BW) of 6.89 ± 0.15 kg were divided into four diets for 28 days. The treatments were basal diet (control), basal diet supplemented with antibiotic (20 mg/kg colistin sulphate and 40 mg/kg bacitracin zinc, AT), probiotics (400 mg/kg Bacillus coagulans ≥5 × 109 CFU/g, BC) or yeast hydrolysate (5000 mg/kg yeast hydrolysate, YH). Average daily gain (ADG) and average daily feed intake (ADFI) were improved by AT and YH diets (p < 0.05), while BC diet only increased ADG (p < 0.05). The complement 3 (C3), lysozyme (LZM) and tumour necrosis factor-α (TNF-α) concentrations in serum were increased in BC diet (p < 0.05). Feeding AT and YH caused the increase of jejunal villus height (p < 0.05), and a higher ratio of villus height/crypt depth was observed in AT, BC and YH groups (p < 0.05). The mRNA expression of zonula occludens-1 (ZO-1) in jejunal mucosa was up-regulated by AT, BC and YH diets (p < 0.05). Dietary AT, BC or YH inclusion decreased the interleukin-1β (IL-1β) concentration and TNF-α mRNA expression (p < 0.05), and YH supplementation even down-regulated toll-like receptor 4 (TLR4) and CD14 expressions (p < 0.05). In summary, the dietary administration of BC or YH both improves growth performance through promoting the intestinal barrier function, indicating both of them can serve as potential alternatives to antibiotics growth promoters for the piglet production.     

猪伪狂犬病的流行病学、临床症状及防控措施

猪伪狂犬病由伪狂犬病毒引起,临床上以妊娠母猪流产、产死胎,新生仔猪出现共济失调、神经症状,并造成仔猪大量死亡为特征。自伪狂犬病毒毒株变异以来,病猪数量不断增加,严重威胁了养猪业健康发展,并造成巨大的经济损失。文章将从流行病学、临床症状、病理变化、鉴别诊断、防控措施等几个方面对猪伪狂犬病进行概述,希望能够对猪伪狂犬病的防控、净化提供帮助。     

Effects of soybean raffinose on growth performance,digestibility,humoral immunity and intestinal morphology of growing pigs

There are appreciable does of raffinose in soybean, but the impacts of raffinose on pigs are poorly investigated. We used 2 experiments to investigate the influence of soybean raffinose on growth performance, digestibility, humoral immunity and intestinal morphology of growing pigs. In Exp. 1, a total of 30 crossbred (Duroc × Landrace × Yorkshire) barrows (21.93 ± 0.43 kg) were randomly divided into 3 groups, and were fed with the control diet, the control diets supplemented with 0.2% and 0.5% raffinose, respectively, for 21 d. Results showed that the addition of 0.2% or 0.5% raffinose reduced (P < 0.05) average daily feed intake (ADFI), average daily gain (ADG) and nutrient digestibility, and dietary 0.5% raffinose increased the ratio of feed to gain (P < 0.05). For serum indexes, dietary 0.5% raffinose decreased growth hormone and increased glucagon-like peptide-2, immunoglobulin G, tumor necrosis factor-α (TNF-α) and interleukin-6 concentration (P < 0.05). In Exp. 2, a total of 24 crossbred barrows (38.41 ± 0.45 kg) were randomly divided into 3 groups, and were fed with the control diet (ad libitum), the raffinose diet (0.5% raffinose, ad libitum), and the control diet in the same amount as the raffinose group (feed-pair group) for 14 d, respectively. Compared with the control diet, dietary 0.5% raffinose decreased ADFI (P < 0.05). Intriguingly, the raffinose group had lower ADG than the feed-pair group, lower nutrient digestibility, lower amylase activity in duodenum, lower amylase, lipase and trypsin activities in jejunum and higher TNF-α concentration in serum compared with the other 2 groups, and a higher ratio of villus height to crypt depth compared with the control group (P < 0.05). These results showed that soybean raffinose could reduce feed voluntary intake and body gain while improving intestinal morphology without a significant negative influence on immunity. Taken together, dietary raffinose could decrease growth performance by reducing both feed intake and nutrient digestibility while inducing humoral immune response of growing pigs.     

Effects of a Dual‐Frequency Frequency‐Sweeping Ultrasound Treatment on the Properties and Structure of the Zein Protein

We investigated the effects of a dual‐frequency frequency‐sweeping ultrasound (DFFSU) treatment on the functional properties and structure of zein. The solubility of ultrasound‐treated zein proteins increased slightly but significantly as the treatment time increased. The results showed that the DFFSU treatment had an obvious influence on the mean particle size and the size distribution. A significant (P < 0.05) increase in the size of the particles with respect to time was observed after a sonication time of more than 20 min in zein solutions. Differential scanning calorimetry results showed that sonication alters the thermal behavior of zein. Circular dichroism spectra showed a small increase in the percentage of ordered structure elements within the protein molecule. After 60 min of ultrasonication, the percentage of α‐helix structures increased by 0.9%, whereas the percentage of β‐sheets and β‐turns decreased by 0.5%. Microstructural analyses by scanning electron microscopy showed that several microholes appeared in the zein following ultrasonic pretreatment. Under the conditions investigated in this study, DFFSU treatment was found to affect the studied functional properties of the zein protein. This technology could be used to obtain improved functional properties in some protein samples.     

2BMC―4/8型棉花双行错位精量穴播机的设计与试验

基于实现黄河流域麦棉连作,针对前茬小麦秸秆量大,麦棉一年两作农时紧张的问题,以及夏棉种植密度大需改善单株夏棉生长空间等要求,设计2BMC―4/8型棉花双行错位精量穴播机。该机能一次实现全幅旋耕整地、侧深施肥、种床镇压、精量穴播、覆土镇压等功能。其不但能实现66+10cm的种植模式,而且能够实现双行种子的交错排列,在保证实现机采棉的前提下,实现高密度夏棉的最优化空间布置。创新设计双轮整平、镇压装置和一体双行错位排种器。田间试验表明,该播种机作业性能达到设计要求。     

寒地包衣玉米种子压缩特性试验研究

以寒地包衣玉米种子龙丹47为研究对象,对其物料特性进行了测量,得到三轴尺寸长9.287mm、宽7.64 mm、厚5.9 8 mm,休止角3 2.2 0°,自流角2 5.4 0°,千粒质量3 6 9.5 g。利用CTM2 0 5 0微机控制电子万能材料试验机,得到龙丹47玉米种子在平放、立放、侧放条件下的压缩特性曲线,经拟合曲线符合PEAL-READ模型,并获得了最大压缩载荷(平放379.5N、立放124.3N、侧放161.926N)、弹性模量(平放161.528 MPa、立放45.66MPa、侧放1 0 2.8 9 3 MPa)和抗压强度(平放1 0.8 4 4 MPa,立放3.5 5 2 MPa、侧放4.6 2 6 MPa)。结果显示:籽粒平放时的压缩载荷明显高于侧放和立放时的载荷,且平放压缩载荷是立放压缩载荷的2.6倍,是侧放压缩载荷的2.7倍;而侧放与立放的压缩载荷差异不大,表明籽粒在侧放和立放时更易受压缩外力的影响。     

Nanosize of zinc oxide and the effects on zinc digestibility,growth performances,immune response and serum parameters of weanling piglets

The aim of this study was to investigate the effect of dietary supplementation of nanosize zinc on zinc digestibility, growth performances, immune response and serum parameters of weanling piglets. Ninety‐six LYD weanling piglets were assigned to control, zinc oxide (ZnO), organic‐Zn (Zn‐methionine) and nanosize ZnO (nano‐Zn) groups with four replicates. The zinc was at the 120 mg/kg level in the treatment group's diet, while the control group's was 80 mg/kg Zn. The experiment results indicated that the nano‐Zn and organic‐Zn groups had significantly higher Zn digestibility compared to the ZnO and control groups. For the immune response traits, the IgG level and goat red blood cells (GRBC) antibody titer were nano‐Zn and organic‐Zn>ZnO>control; in the phytohemagglutinin (PHA) challenge test result, nano‐Zn>organic‐Zn>ZnO>control; in regard to the γ‐globulin level, nano‐Zn and organic‐Zn>ZnO and control, with significant difference between groups. In the serum parameters aspect, serum Zn concentration in nano‐Zn and organic‐Zn groups were higher than in the ZnO and control groups, serum growth hormone concentration was increased in the nano‐Zn group than in the other groups. In conclusion, nanosize zinc oxide for dietary supplementation can increase zinc digestibility, serum growth hormone levels and carbonic anhydrase activity and enhance the immune response of weanling piglets.     

桃在低温贮藏过程中品质变化研究

为比较不同类型桃在低温条件下的贮藏特性,以水蜜桃‘美香’和蟠桃‘银河’为试材,研究了低温贮藏过程中果皮颜色、果实硬度、可溶性糖和有机酸含量等的变化。结果表明:低温贮藏期间桃果皮颜色的变化在不同品种间表现有较大差异,‘银河’在贮藏过程中果皮颜色略微加深;2个品种果实硬度总体下降比较明显;不同品种果实可溶性糖含量的变化比较复杂,其中果实可溶性糖总量波动较小,蔗糖含量在贮藏后期明显下降;有机酸总量方面,‘美香’和‘银河’总体都呈现前期下降,至贮藏后期小幅上升的变化趋势,其中柠檬酸的含量在贮藏后期出现明显下降。     

动物防疫领域政府购买公共服务相关术语混合使用情况辨析

自1994年深圳市罗湖区在环境卫生领域率先引入政府购买公共服务以来,我国政府购买公共服务经历局部性试点与探索、试点推进、制度化推广、全面推开与创新等4个阶段。2005年以来,我国对动物防疫领域政府购买公共服务进行了探索,但有关术语及其涵义一直未界定,更未形成共识,导致术语混用较为严重。术语混用体现了人们认知不同,看法不一,给动物防疫领域政府购买公共服务理论研究和实践探索造成了混乱。鉴于此,本文尝试梳理术语混用的背景、混用情况并予以辨析,以探讨动物防疫领域政府购买公共服务相关术语的正确使用。     

Synthetic Application for Analysis Methods of the Planar Mechanism Overconstraints

Overconstraints exist widely in planar closed chain mechanisms and mechanical systems result in a series of detrimental influences on behavior of machines, and attention is given to them by machine designer. Some analysis methods of overconstraints in planar closed chain mechanisms are presented, and more study due to the faultiness of these methods. In accordance with faultiness of two common kind of methods, formulas method and loop analysis method of degrees of freedom, and by combining these two methods, an useful mechanism overconstraints analysis method which is convenient for computer aided overconstraints analysis is gained via research on the two methods thoroughly and systematically, and some new views about mechanism overconstraints analysis are presented.