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51.
Madoka ICHIKAWA-SEKI Azirwan GUSWANTO Puttik ALLAMANDA Euis Siti MARIAMAH Putut Eko WIBOWO Yoshifumi NISHIKAWA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2016,78(1):121-123
Neospora caninum can cause fetal abortion and neonatal mortality in
cattle, and is a cause of economic concern worldwide. This study aimed to determine the
prevalence of Neospora caninum-specific antibodies in cattle from Western
Java, Indonesia. Serum samples from 991 cattle from 21 locations were tested for
antibodies to N. caninum by using an enzyme-linked immunosorbent assay
(ELISA) on the basis of recombinant NcSAG1. The overall seroprevalence was 16.6%, ranging
from 0 to 87.5% in the sampled locations. The results of this study indicate latent
infection rates of sampled animals were different in each location. Further studies are
necessary to elucidate the relationship between N. caninum infection and
abortion in cattle, and to identify risk factors for infection in high-prevalence
environments. 相似文献
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A cDNA encoding the Babesia bovis 12D3 antigen homologue was obtained by immunoscreening the expression library prepared from Babesia gibsoni merozoite mRNA. The complete nucleotide sequence of the gene was 1406 bp. Computer analysis suggested that the sequence contains an open reading frame of 1052 bp encoding an expected protein with a molecular weight of 36kDa. Based on homology analysis, this putative protein was designated as the B. gibsoni 12D3 antigen (Bg12D3). The Bg12D3 gene was expressed in the Escherichia coli BL21 strain, and the chronically infected dog serum reacted with the recombinant protein. The antiserum against the recombinant Bg12D3 protein can recognize a 38-kDa native protein, which is consistent with its expected size. Moreover, the purified recombinant proteins were used as the antigen to detect the antibody response in an experimentally infected dog by the enzyme-linked immunosorbent assay (ELISA). Our results indicated that the Bg12D3 protein was recognized by the host immune system and that it induced an antibody response in chronic B. gibsoni infection. These results allowed us to identify a new member of the 12D3 antigens and its characteristic immune response in canine B. gibsoni infection. 相似文献
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Analysis of the nitrogen nutrition of soybean plants with deep placement of coated urea and lime nitrogen 总被引:1,自引:0,他引:1
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Kazuyuki SUZUKI Nao KONDO Kaede TAKAGI Akitoyo NISHIKAWA Yoshiki MURAKAMI Marina OTSUKA Kenji TSUKANO Keiko IKEDA Hisashi FUNAKURA Ichiro YASUTOMI Satoshi KAWAMOTO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(5):767
Point-of-care (POC) devices that veterinary practitioners can use to easily and rapidly measure blood ionized calcium (iCa) levels in cows immediately after withdrawing a blood sample on the dairy farm are needed. Aims of present studies was to compare the commercially available ion-selective electrode handheld iCa meter (bovine blood iCa checker) with the benchtop blood gas analyzer GEM premier 3500 and handheld analyzer i-STAT 1. Sixty-two paired-point whole blood samples were obtained from three cows with hypocalcemia experimentally induced by Na2-EDTA infusion. Whole blood samples were also obtained from the 36 cows kept on a farm in field conditions. The results using the bovine blood iCa checker correlated with those using the GEM premier 3500 and i-STAT 1. Bovine blood iCa checker was “compatible” with the GEM premier 3500 and i-STAT 1 because the frequency of differences between the measurements within ± 20% of the mean were 100% (65/65, >75%) and 90.8% (59/65, >75%), respectively. In the field trial, the blood iCa concentration measured by the bovine blood Ca checker was significantly positively correlated with that measured by the i-STAT 1 portable analyzer. Bovine blood iCa checker was “compatible” with the i-STAT 1 because the frequency of differences between the measurements within ± 20% of the mean was 100% (36/36, >75%). Results from these findings, the bovine blood iCa checker may be applied as a simplified system to measure the iCa concentration in bovine whole blood. 相似文献
59.
Wenxi CHEN Tetsu NEMOTO Toshio KOBAYASHI Toshiyuki SAITO Etsuko KASUYA Yoshifumi HONDA 《Animal Science Journal》2002,73(6):545-551
A method of recording the fetal electrocardiogram (ECG) and heart rate (HR) is described. An abdominal lead and a thoracic lead were used to measure the fetal ECG and maternal ECG, respectively. The maternal component in the abdominal lead measurement was estimated by a digital adaptive filter using the thoracic lead measurement as a reference. By suppressing the estimated maternal components in the abdominal lead, the fetal ECG could be detected. The beat‐by‐beat fetal QRS complex peaks were determined by a digital matched filter from the fetal ECG enhanced in this way. The method was trialed in 10 pregnant Holstein cows at gestational periods ranging from 136 to 224 days. The results show that this method can extract the fetal ECG and determine the fetal HR from the raw noisy measurement data. The results suggest that the method would have applicability in monitoring fetal ECG as well as providing a non‐invasive, continuous HR profile during gestation, which will enable better understanding of the development of cattle fetuses before delivery. 相似文献
60.
Yu L Zhang S Liang W Jin C Jia L Luo Y Li Y Cao S Yamagishi J Nishikawa Y Kawano S Fujisaki K Xuan X 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(11):1509-1512
An epidemiological survey on a Theileria parasite infection of cattle in Northeast China was carried out using allele-specific PCR and DNA sequence analysis of the major piroplasm surface protein (MPSP) gene. The results showed that 14 of 104 blood samples were positive for Theileria by PCR. Among the positive cases, co-infection with various combinations of C- and I-type parasites was detected in 12 samples; no B- and Thai-type parasites were detected by allele-specific PCR. Phylogenetic analysis based on the MPSP gene sequences revealed that Theileria parasites with the MPSP types 1, 2, and 4 were distributed in Northeast China. 相似文献