猪胃肠道微生物菌群的研究现状及调控技术进展

动物正常的消化吸收与其胃肠道内的微生物菌群有着直接的联系,而胃肠道微生物菌群与宿主胃肠道环境和宿主所处的外界环境密切相关,形成统一的整体.论文就猪胃肠道微生物菌群的组成和分布、胃肠道微生物菌群的作用、影响猪胃肠道微生物菌群的因素、动物胃肠道微生物菌群的研究手段及猪胃肠道微生物菌群的调控等五个方面进行综述,对进一步研究猪...     

药用植物红大戟快速繁殖技术研究

对药用植物红大戟快速繁殖的研究进行了详细对比分析,指出在不同快速繁殖方法中存在的问题和不足,提出与快速繁殖关键技术相关的建议,为红大戟种质资源保护和规范化种植奠定理论基础。     

农村运输机械耗油量数学模型的研究

燃油消耗是农村运输机械作业的主要消耗项目,通过对农村运输机械复杂的运输作业过程的分析,提出了农村运输机械年平均运程及其回程满载、空载的等效关系模型,进而推出了综合反映运输条件特性和运输机械结构条件参数的农村运输机械耗油量数学模型,并经实例试验计算证实了模型的适用性.     

四川旱耕地生产、生态问题及水土流失综合治理研究

四川地域辽阔 ,地貌以山地和高原为主 ,农耕地分布范围广 ,旱耕地比重大 ,水土流失和与之相关的旱、洪灾害等生产、生态问题突出 ,既导致农作物产量低而不稳 ,又给长江中下游带来安全隐患 ,因此 ,四川在实施西部大开发战略中 ,除实施天然林保护工程外 ,旱耕地 ,特别是旱坡耕地 (2°～ 2 5°)的水土流失综合治理 ,既具有极其重要的战略位置 ,也是四川农业持续发展不能忽视的重大问题     

褐盔蜡蚧在葡萄上的发生规律及防治技术

褐盔蜡蚧在葡萄上危害葡萄枝条、叶片和果实。一般成、若虫固定后刺吸汁液,排出大量粘液,滋生黑色霉菌,污染果面和叶片,影响光合作用。该虫在甘肃武威地区1a发生2代,以幼虫和卵在枝条皮缝中越冬。施用10%吡虫啉可湿性粉剂1000倍液,虫口减退率可达99.5%,4.5%高效氯氰菊酯乳油1500倍液,虫口减退率为94.4%。     

TLC-UV分析测定南昆山白毛茶生物碱成分

以市售五个普通茶叶样品为对照，采用薄层色谱分离白毛茶的多种生物嘌呤碱．以乙酸乙酯：丙酮：氨水=4：3：1（V：V)为流动相，硅胶G为固定相，展开浸取后。用紫外分光光度法在273nm处测定其成分含量．结果表明：南昆山白毛茶具有特殊性。其生物碱种类及成分结构异于普通茶叶。含有丰富的可可碱．高达3.02～3.15％。比普通对照样高出11．7～13．2倍．总生物碱量为4．68～4．89％．     

Cystourethropexy to Correct Refractory Urinary Incontinence Due to Urethral Sphincter Mechanism Incompetence Preliminary Results in Ten Bitches

Cystourethropexy was performed in 10 bitches with refractory urinary incontinence due to urethral sphincter mechanism incompetence. All animals had an abnormally shaped vesico-urethral junction. The bladder neck was located in the pelvic canal in nine dogs (pelvic bladder). Surgery alone restored urinary continence in two cases and markedly improved incontinence in two cases. Surgery combined with medical therapy (phenylpropanolamine, 1.5 mg/kg orally once or twice a day, using a sustained-action preparation) restored urinary continence in four cases and markedly improved continence in one case. Surgery, with medical therapy, was completely ineffective in one case. No major complications were encountered in any dogs. In four cases, histopathologic evaluation of the dorsal bladder wall and neck revealed the presence of a single, perinuclear, clear vacuole in the cytoplasm of smooth muscle fibers. Electron microscopic examination showed that the vacuoles represented a dilatation of normal membrane organelles. Such abnormalities have the potential to alter the smooth muscle motility. This study indicates that cystourethropexy alone restored urinary continence only temporarily in the majority of the patients. The significance and frequency of the histopathologic findings requires further investigation.     

An experimental exploration of focal inflammatory response in cerebral ischemic foci in the rat

AIM: To study the pathological relationship of vascular cell adhesion molecule-1 (VCAM-1) expression and monocyte/macrophage infiltration with focal brain ischemia. METHODS: Immunohistochemical technique and focal brain ischemia/reperfusion model were used in the study in order to explore profiles and time-course of VCAM-1 expression and monocyte macrophage (ED2 positive cell) infiltration in ischemic rat brain. RESULTS: VCAM-1 was up-regulated in microvascular endothelial cells in ischemic cortex at 1h postischemia, and continuously expressed during the time of reperfusion. ED2 positive cells infiltrated into ischemic cortex at 1h iscehmia/ 2h reperfusion and then ED2 positive cells increased gradually with the time of reperfusion, ED2 positive cell infiltration showed apparently relationship with VCAM-1 expression, and both of them exhibited the some changes of time-dependence. CONCLUSION: Cerebral ischemia induced VCAM-1 expression and ED2 positive cell infiltration and VCAM-1 may regulate the recruitment of ED2 positive cells in the ischemic brain region. The results suggested that VCAM-1 and ED2 positive cells may be participated in the pathogenesis of cerebral ischemic injury.     

Effects of oxidized low density lipoprotein and vitamin E on the levels of IL-6,IL-8 and TNF-α in cultured human umbilical vein endothelial cells

AIM:To investigate effects of OX-LDL and VitE on the levels of IL-6,IL-8 and TNF-α in human umbilical vein endothelial cells(HUVEC).METHODS: Human umbilical vein endothelial cells were obtained by in vitro culture. HUVEC treated with or without Vit E was incubated with OX-LDL, and the levels of IL-6, IL-8 and TNF-α were determined by enzyme-linked immunosorbent assy technique. RESULTS:50 μg/L,100 μg/L, 200 μg/L OX-LDL induced the release of IL-6,IL-8 and TNF-α by HUVEC in a dose-dependent manner. Compared with the control group , the levels of IL-6 and IL-8 were significantly increased at 6-12 h of stimulation with OX-LDL . Maximal levels of IL-6 and IL-8 occurred after 24-36 h, reaching a plateau maintained for at least 48 h. TNF-α rose after 2-6 h in HUVEC, and reached a maximum after 12 h. In contrast to IL-6 and IL-8, TNF-α declined after 48 h. However, when VitE (50 mg/L,100 mg/L,200 mg/L)was added, it can significant inhibited the release of IL-6, IL-8 and TNF-α in a dose-dependent manner, and after 48 h these cytokines have no diference between OX-LDL+VitE groups and OX-LDL groups. CONCLUSION: OX-LDL can obviously stimulate the production of IL-6,IL-8 and TNF-α in vascular endothelial cells, which can significantly be inhibited by VitE in a short time.     

塔里木河干流河道生态需水量研究

基于有序聚类法,在对1957-2006年塔里木河干流径流序列受人类活动显著影响突变点判别的基础上,对干流新渠满、乌斯满和恰拉3个水文断面河道逐月最小生态需水量和适宜需水量进行计算。结果表明：① 近50 a来,人类活动对干流水资源影响程度不断增大,1976年前的径流量可以近似看作自然状态;② 新渠满、乌斯满和恰拉河道年最小需水量分别为17.68×10⁸ m³、10.02×10⁸ m³和3.29×10⁸ m³,年适宜需水量分别为35.65×10⁸ m³、18.23×10⁸ m³和7.00×10⁸ m³;③一般用水期（10月至翌年3月）保证新渠满、乌斯满和恰拉河道最小需水量5.33×10⁸ m³、3.77×10⁸ m³和1.32×10⁸ m³,用水敏感期（4-9月）保证适宜需水量28.99×10⁸ m³、13.70×10⁸ m³和5.09×10⁸ m³即可确保干流河道生态系统健康。