Influence of allogeneic cornea on human peripheral blood T lymphocyte subtype in vitro

AIM: To observe the immunoregulation of allogeneic cornea on the human peripheral blood T lymphocytes in vitro. METHODS: After Co-culture of human peripheral blood lymphocytes and allogeneic cornea in vitro, T lymphocytes were labeled by monoclonal antibody, and analyzed by fluorescent activated cell sorter (FACS). RESULTS: CD25 expression on T lymphocytes in control was 25.2%, after stimulated by the allogeneic cornea or PDB, CD25 expression on T lymphocytes was 56.8% and 80.9%, respectively. After stimulated by the allogeneic cornea, CD25 expression on CD4+ or CD8+ T lymphocytes were 67.3% and 52.3%, respectively. CONCLUSION: Allageneic cornea stimulates CD25 expression on human peripheral blood T lymphocytes, and the CD25 expression on CD4+ T lymphocytes is more prominent than CD8+ T lymphocytes.     

Establishment and biological characteristics of a new human endometrial carcinoma

AIM: To establish a new human endometial carcinoma cell line for basic and clinical study. METHODS: The minced tumor tissues were inoculated subcutaneously into the back of nude mice. After forming solid tumor, the mice were killed and the transplant was taken out for primery culture. RESULTS: The established cell line was maintained by serial passages, named EAC. EAC cell line grew rapidly,steadily, and it had similar microscopic morphology to adenocarcinoma cell. Chromosome analysis reveal EAC chromosome counts ranging in hyperdiploid. The expression of ER, PR, P53, MDM2 were negative by SP immunohistochemistry. Tumorigenicity of EAC in nude mice was 100%. CONCLUSION: A new human endometrial carcinoma, EAC, is established successfully, which is helpful for the basic and clinical study of endometrial carcinoma.     

Expression of TNF-α and TGF-β in oral lichen planus patients

AIM: To investigate the expression of TNF-α， TGF-β in patients with oral lichen planus (OLP) and normal oral mucosa (NOM). METHODS: An immunohistochemical technique was performed to detect TNF-α, TGF-β1 and TGF-β2 expression in 22 cases with OLP and 10 normal controls. RESULTS: In lamina propria of OLP, the expression of TNF-α and TGF-β1 were increased, whereas TGF-β2 did changed significantly compared with control group. TNF-α positive signal were mostly found in macrophages, lymphocytes. TGF-β1 positive cell was present in macrophages, endothelial cells and fibrocytes. CONCLUSION: TNF-α and TGF-β1 play an important role in the development and maintenance of OLP local inflammation.     

鸡骨型白血病免疫琼扩与放射摄片诊断的比较

为了探索特异性免疫学诊断方法对鸡骨型白血病(OP)的检测效果。用双盲法分批采样同时进行羽髓琼脂扩散试验和胫骨X线摄片检查对比,共检834只鸡(433羽属天然自发OP鸡群,401羽属人工诱发实验鸡群)。结果羽髓琼扩试验的OP阳性检出率为3．48％(29／834：)。阳性病理符合率为75．86％(22／29)。阳性误诊(假阳性)率24．13％(7／29),阴性误诊(假阴性)率36．15％(291／805)。而X线摄片检查的OP阳性检出率为38．37％(320／834)。X线阳性完全可获病理组织学证实,不存在假阳性或假阴性。把禽白血病羽髓琼扩试验试用于检测鸡骨型白血病尚属初次报道,其阳性检出率低而误诊率高,尚不宜作OP检测手段。放射摄片诊断的阳性检出率高而误诊率低,可快速诊断,故适用于OP检疫。     

深圳草坪越冬保绿的施肥研究

研究比较了4种肥料在深圳3个草坪草上对促进草坪草的生长和越冬保绿效果。结果表明,施肥处理对台湾草、马尼拉草和假检草均有明显的促进生长和越冬保绿效果。其中,施用有机无机草坪肥和草坪液肥的应用效果较其他两种肥料更好。     

小灵猫定点排粪及即时取食的驯化研究

为探索小灵猫人工驯养繁殖技术,对小灵猫生态行为进行观察研究,结果表明,小灵猫的某些野性可通过人为引导加以改进,向有利于人工养殖的方面发展,并成功地引导其定点排粪及投食后即时取食。     

貉群暴发犬瘟热的诊断

为了对2006年7~9月间山东、河北的多个地区貉群发病情况进行诊断,根据临床症状,剖检变化,实验室诊断,确诊为犬瘟热病。     

18S rRNA／rDNA序列分析技术在瘤胃原虫分类学研究中的应用

作者指出了反刍动物瘤胃原虫分类学研究中的难点，介绍了原虫分类学中新兴的分子生物学指标(18S rRNA／rDNA序列同源性)，综述了18S rRNA／rDNA序列分析技术在瘤胃原虫分类研究中的应用。     

利用巢式PCR快速鉴定牛传染性鼻气管炎

为了快速检测牛传染性鼻气管炎,采用SDS-蛋白酶K法,提取病毒模板DNA。根据IBRV gB基因序列设计了1对特异引物,在其上下游引物的内侧又分别设计了1对引物,以这4条引物对IBRV模板进行扩增。结果成功扩增出预期目的片段,建立了巢式PCR检测方法。敏感性、特异性等检测试验结果表明该方法能特异检测IBR病毒。本方法具有快速、灵敏、特异的优点,适用于在牛及其遗传物质的进出口检疫中进行牛传染性鼻气管炎快速病原鉴定。     

猪用发酵床的研究与应用

新型微生物发酵床养猪技术是一种新兴的无公害养猪技术,它可有效地解决猪生产中的粪便处理和环境污染问题,改善猪的福利状况,利于猪只的生长和发育,其环保性和经济性明显优于集约化,规模化养猪方式。本文介绍了微生物发酵床的概念、分类和工艺流程,讨论了其技术要点和应用效果,并对其发展前景进行了展望。