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51.
ZHOU Qi LI Shao-qiang LIANG Li-jian PENG Bao-gang ZHEN Yu-yang SU Cai-kun 《园艺学报》2006,22(7):1348-1352
AIM:To detect the mRNA expression of tissue factor (TF), urokinase-type plasminogen activator (u-PA) and urokinase-type plasminogen activator receptor (u-PAR) in the samples of hepatocellular carcinoma (HCC) and adjacent tissue, and to elucidate their association with clinical significance. METHODS:The mRNA levels of TF, u-PA and u-PAR in 27 human HCC tissue samples with their adjacent tissue samples and 27 normal liver tissues were detected by RT-PCR. The relationship between the mRNA expression and their clinic-pathological data were also analyzed. RESULTS:The expressive rate of TF, uPA and uPAR in HCC tissue samples was 62.96% (17/27), 70.37% (19/27), 77.78% (21/27), respectively and relative expression intensity of TF, uPA and uPAR were 0.567±0.268, 0.964±0.458 and 0.784±0.322, respectively, which were significantly higher than those in adjacent tissue group and normal liver group (P<0.05). TF, uPA and uPAR mRNA levels were all significantly higher in larger size and in the invasive and metastatic subgroup (P<0.05). There had positive relationship between the mRNA expressions of these three factors. CONCLUSION:The results indicate that TF, uPA and uPAR might play an important role in hepatic carcinogenesis, invasiveness and metastasis. 相似文献
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David White Weidong Chen 《European journal of plant pathology / European Foundation for Plant Pathology》2007,119(1):3-12
Ascochyta blight is a serious disease of cool-season grain legumes (chickpea, faba bean, lentil and pea) caused by fungal
species of the anamorphic genus Ascochyta and related genera. Despite extensive studies on the biology, ecology, epidemiology
and management of the disease, little is known about the pathogenic determinants of these pathogens. This research aims at
using Ascochyta rabiei as a model for the genus in investigating genetic factors of pathogenicity, with the ultimate goal of elucidating pathogenic
mechanisms. Three advances were made: (1) insertional mutants with altered pathogenicity were identified through in vivo screening,
and genomic regions adjacent to the insertion sites in selected mutants were determined; (2) a phage library of A. rabiei genomic DNA was constructed, and the library was estimated to provide complete coverage of the A. rabiei genome. This library was used successfully to recover clones with DNA adjacent to insertional mutation sites and to isolate
specific genes; (3) DNA probes specific for an acyl-CoA ligase (cps1) and a polyketide synthase gene (pks1) were developed and library clones containing the corresponding genomic regions were identified from the phage library.
These advances provide the foundation and necessary tools for experimentation of ectopic complementation assays and targeted
mutagenesis to elucidate the genetic mechanisms of pathogenicity of A. rabiei. 相似文献
55.
遮光对青榨械光合速率及叶绿素荧光参数的影响 总被引:3,自引:0,他引:3
研究了遮光15%、35%、50%和全光照处理对青榨槭新梢生长、叶片色素含量、净光合速率以及叶绿素荧光参数的影响。结果表明,随着遮光度增加,叶片色素含量增加,叶绿素a/b减小;叶片净光合速率(Pn)、初始荧光(Fo)、原初光能转化效率(Fv/Fm)和光化学猝灭系数(qP)增加,但非光化学猝灭系数(qN)降低。全光照和遮光15%处理条件下,Pn日变化呈双峰曲线,而35%和50%处理条件下呈单峰曲线;遮光35%和50%处理条件下Fv/Fm日变化幅度较小,15%和全光照处理条件下Fv/Fm日变化呈先上升后下降再上升趋势。50%遮光处理青榨槭幼苗Pn最高,新梢年生长量最大,可在育苗生产中应用。 相似文献
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罗布泊南部野双峰驼的分布与环境 总被引:5,自引:0,他引:5
在1995-1996年两年调查的基础上,我们又利用家驼队深入荒漠腹地,考察了罗布泊南部野双峰驼的分布和栖息环境,结果表明在罗布泊南部的湖岸区,阿尔金山北麓及山前丘陵地带均有野驼分布,库姆塔沙漠中部无野驼进入,但其西侧戈壁及丘间谷地为野驼南北迁徒的通道,冬季野驼主要集中活动在科什兰孜等罗布泊南岸及阿奇克谷地;夏季主要在山区北麓活动,所以应将该区域与上述分布区同归于一个分布区整体,即南北配置的景观复合 相似文献
59.
氟罗沙星对禽败血霉形体(Mycoplasma gallisepticum)病的药效研究 总被引:1,自引:0,他引:1
体外抑菌试验测得氟罗沙星(Fleroxacin)对禽败血霉形体(Mycoplasmagalisepticum)的最小抑菌浓度(MIC)为0.015mg/L。体内药效试验表明,氟罗沙星以25、50、100mg/L饮水给药和以5、10、15mg/kg肌注给药(1次/d),连续用药5d,对人工气囊接种禽败血霉形体培养液(0.2mL,约含108cfu)的雏鸡,保护率均为100%(30/30),而感染不给药组雏鸡存活率为93.3%(28/30);相对增重率分别为90.8%、91.5%、92.3%和91.5%、91.8%、92.9%,显著高于感染不给药组(73.2%);气囊损伤分分别为2.93、2.62、0.93和1.8、1.1、1.0,而感染不给药组为6.57,差异显著(P<0.05);血清玻板凝集试验阳性率分别为20%(2/10)、10%(1/10)、0(0/10)和30%(3/10)、10%(1/10)、10%(1/10),均极显著低于感染不给药组100%(10/10)(P<0.01)。氟罗沙星不同给药途径及不同剂量间药效差异不显著。 相似文献
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