甜菜组培快繁及植株再生的研究

以甜菜8个品系种子、幼苗的茎节、茎尖、叶片(带叶柄)、腋芽等作外植体,对甜菜快繁、植株再生以及再生植株的移栽等技术进行了研究.结果表明,不同外植体在MS+6-BA 0.8～1.5mg/L+NAA 0.2～0.3mg/L培养基上,诱导效果最佳,而且幼苗生长健壮.生根培养在MS+NAA 1.0～1.5mg/L或MS+NAA 1.0～1.2mg/L+IBA0.2mg/L上,生根率可达85%以上,且根系发达,再生植株的移栽成活率达90%以上.建立了甜菜组培快繁及植株再生的技术体系,同时该再生体系可作为基因转化的受体系统.     

河套灌区瓜类作物间作油用向日葵技术模式研究

通过对巴彦淖尔市黄河灌区瓜类(西瓜、蜜瓜、籽瓜)平作与开沟两种栽培模式下间作油用向日葵产量、产值的分析.结果表明,瓜类作物通过开沟模式结合间种油用向日葵是提高单位面积产值的有效手段,应进行大面积推广种植.     

新疆棉花高体细胞胚胎发生能力基因型的筛选

本研究以15个新疆主栽优良品种(系)以及两个对照品种YZ-1和Y668为研究对象,利用下胚轴做外植体进行胚胎发生能力的比较研究,筛选出了5个能够胚胎发生的基因型,即新陆早12,新陆早17,新陆早22,新陆早32和新陆早33,其中新陆早32和新陆早33,能在3个月左右分化,且分化率较高,可达90%左右,其它3个品种的分化效率为:新陆早17为46.9%,新陆早12为35.1%,新陆早22为25.3%,但这3个品种的分化时间都比较长,需5个月左右.同时对5个再生品种及两个对照品种的成胚效率进行了比较,由新陆早32和新陆早33诱导的胚性愈伤在胚状体数目及胚胎萌发效率都比较高,具备作为棉花优良遗传转化受体的基本特征,目前已经用于农杆菌介导的转基因试验.上述研究拓展了棉花组织培养及转基因的受体范围,为新疆棉区转基因育种研究工作的开展提供了重要基础材料.     

葡萄结果母枝粗度与新梢结实力的关系

研究了11个葡萄品种结果母枝粗度与新梢结实力的关系,结果表明,不同葡萄品种获得理想的新梢结实力,其结果母枝须有一适宜的粗度范围;结果母枝粗度与结果枝率、结果枝结果系数、新梢结果系数之间呈二次多项式回归关系;不同葡萄品种冬季修剪时结果母枝的选留应以不同粗度结果母枝的结实力为依据。     

蛭石压盖粮面安全储粮研究

粮面压盖储粮技术能有效地降低储粮温度的上升幅度,我库在13号仓采用蛭石进行粮面压盖试验,试验仓的表层粮温和最高温度比对照仓低5~7℃,能使粮堆长期保持低温状态,取得了很好的效果。粮堆的自身呼吸作用降氧,使粮堆处于低氧状态。采用膜下磷化氢低剂量(3 g/m)投药,使粮堆内长期保持有效磷化氢浓度,达到了“三低”储粮环境,起到了隔热保冷,防止害虫繁衍,减少对粮食的污染和延缓粮食品质陈化的作用。     

茂名大放鸡海域文昌鱼分布与种群特性研究

研究表明茂名大放鸡海域的文昌鱼主要分布在东经111°10′～111°17′,北纬21°19′～21°27′区域,分布面积达195．5km2,平均资源密度为51～86尾／m2,最高达到815尾／m2,文昌鱼分布密度中心概位在小放鸡西至西北侧附近;体长结构具明显的季节变动,秋冬季节是文昌鱼的主要补充季节;性腺成熟度和性别比例在不同季节和生长阶段具有明显的差异,春季性成熟个体占的比重最高达65％;随着体长的增加文昌鱼性腺成熟度也渐次增加,体长与性腺成熟度比例成三次曲面关系;其性比随着体长增长发生明显变化,体长为35～60mm时雌雄个体性比接近1,体长大干60mm时雌性个体数量明显小于雄性;文昌鱼全年的体长体重关系方程为W=0．0012L3.32;当体长达到50mm后文昌鱼资源群体尾数减少系数为0．55／cm。     

Effect of Potassium Nutrition of Different Varieties of Rice on the Redox Status in Microzone Rhizosphere Soils

Being divided into three groups-strong,moderate and weak-according to the different kinetic parameters (Fmax,km,Cmin) of potassium uptake by crops,21 cultivars of rice have been studied to find out the relationships between their potassium nutrition and the oxidation-reduction status in the rhizosphere soils. Results show that,with no application of K fertilizer,there were higher contents of active reducing substances and ferrous iron in rhizosphere soils planted with cultivars,such as Zhongguo 91,week in absorbing potassium than in soils cropped with cultivars,Shanyou 64,stronger in absorbing potassium.As a result of K application,however,these toxic substances were decreased appreciably in the soil,particularly in the root zone where weakly K-absorbing sultivars were growing,and the parameter of soil redox (pH pE) was increased,the most striking example of this being found in the rhizosphere soil where the more strongly K-absorbing sultivars were growing.On and close to the root surface in soils where rice plants were supplied with potassium fertilizer,rather more iron oxide had been accumulated compared with rice receiving no potash,and even greater amounts of red iron oxide precipitated on the rice root in neutral paddy soils.As shown by the concentration distribution of active reducing substances and ferrous iron in a microzone of the profile,the redox range of rice roots supplied with potassium may extend as far as several centimeters from the root surface.It can thus be seen that potassium nutrition exerts its effect first on the morphological properties of rice roots and their exudation of oxygen,then on the content of soluble oxygen and the count and species of oxygen-consuming microbes in the rhizosphere soil,and finally on the redox status of the soil.     

FISH技术在大白菜初级三体额外染色体鉴定中的应用

植物三体是非常重要的遗传材料,在基因、连锁群和分子标记的染色体定位及染色体工程育种等研究中有着十分重要的作用(程祝宽等,2000),但采用传统的核型分析方法     

Incorporating parameter uncertainty into prediction intervals for spatial data modeled via a parametric variogram

In spatial predictions, researchers usually treat the estimated theoretical variogram parameters as known without error and ignore the variability of the parameter estimators. Although the prediction is still unbiased, the prediction error is usually underestimated. Therefore, the coverage probability of the prediction interval usually is lower than the nominal probability. A simulation study is performed to show how the coverage probability for prediction relates to the true range and sill of an exponential variogram. This article proposes two parametric bootstrap methods to incorporate the variability of the corresponding parameter estimators. A simulation study is performed to evaluate the coverage probability of these proposed methods. Finally, we apply the parametric bootstrap methods to a real dataset and compare the results with those from naive (i.e., treating estimated parameters as known) and Bayesian methods.     

关于森林资源可持续经营的几点意见

根据黑龙江省森工林区森林资源现状,并结合该林区一些林业局改革森林资源经营培育措施的经验,探讨了林业可持续经营的出路和办法。