微卫星DNA标记在番茄亲子鉴定中的应用

采用242对微卫星DNA标记引物分析了29份番茄自交系的遗传多态性,从中筛选出扩增带型清晰、稳定的34对引物,34对引物的平均等位基因数为4.53,平均多态信息量(PIC)为0.514 1,累积鉴别力(CDP)达0.999 999.利用其中的11对SSR引物对杂交种‘浙杂210’、母本‘01-199-1-1-0’及7个...     

普通油茶种子4个发育时期的转录组分析

我国油茶产业迅速发展,种苗繁育及栽培技术不断进步,但油茶分子基础研究薄弱,决定油茶产量、茶油质量、抗性等指标的重要经济、生长性状的分子机理研究甚少,这必将阻碍油茶产业的可持续发展.本项目首次采用高通量测序技术solexa技术对普通油茶"长林4号"无性系种子发育的4个时期转录组进行测序,经组装分析获得80 310条uni...     

CCD radiation temperature measurement method for hot continuous casting billet surface

According to the present research status and existing problems of hot continuous billet surface temperature non contact measurement technology, a real time temperature measurement model, which combines two-color(R,G) and monochrome (B) of surface radiation image, is established. The surface image is collected through tricolor charge coupled device (CCD) sensor, at the same time, the temperature dynamic response range of CCD can be improved by controlling integrated time and setting different apertures. Through computer image process technology, multi-point temperature can be measured simultaneously, and 2-D temperature field distribution on continuous casting billet surface can be realized. It is be proved by theory and application that the model and method can provide a theory and technology support for realizing surface temperature field on-line measurement for continuous casting billet.     

A fuzzy logic multicasting algorithm for ad hoc networks

To increase the multicasting efficiency of Ad hoc networks, a fuzzy logic multicasting algorithm (FLMA) is proposed. FLMA adopts the fuzzy logic to tolerate the imprecise information caused by dynamic network topology. The two input variables of the fuzzy logic system are the relative degree of the additional coverage node number and the relative degree of the residual energy. The deferring time of rebroadcast is the output variable of the fuzzy logic system, which is used to optimize the priority of the nodes to rebroadcast. FLMA reduces the redundant retransmission and the chance of the contention and collision, while balances the energy consumption of the nodes. Simulation results reveal that the FLMA achieves better performance than BCAST in terms of the network lifetime, average end-to-end delay, the average number of drops per node and the throughput.     

基因型及外源因子对小麦花药培养一步成苗的影响

研究了不同基因型及几种外源因子在小麦花药培养一步成苗中的效应。结果表明 ,花药培养一步成苗的愈伤组织诱导率、绿苗分化率、白苗分化率及绿苗产量存在基因型间的差异。高蔗糖浓度有利于获得高频率的花药愈伤组织 ,但同时又提高了花药培养中白化苗频率。动力精 (KT)对花药愈伤组织诱导率影响的差异不大 ,但对绿苗分化率、绿苗产量及绿苗 /白苗 (G/ W)均有显著影响。在试验浓度范围内 ,随 KT浓度的增大 ,绿苗产量提高 ,绿苗分化率明显增加 ,而白苗分化率下降 ,因而 G/ W值明显增大。说明 KT主要是通过提高绿苗分化率和降低白苗分化率 ,增大 G/ W而提高花培一步成苗效率的。在与 KT的配合使用中 ,2 ,4 - D比 NAA更有利于提高小麦花药培养中的愈伤组织诱导率和苗分化频率     

肉牛混合日粮不同进食水平对尿中嘌呤衍生物排出量的影响

为研究肉牛不同进食水平对尿中嘌呤衍生物(PD)排出量的影响,试验选用4头当地黄牛按4×4拉丁方设计进行两期消化代谢试验,两个试验的日粮由精料和粗料由40:60(试验1)和60:40(试验2)组成,试验日粮分别按"自由采食量"的100%、80%、60%和40%饲喂4种相同组分不同进食量的日粮,用全收粪法和全收尿法分别测定有机物(OM)的消化率和尿中PD排出量,试验结果表明,日粮进食水平对全消化道OM表观消化率没有明显影响(P>0.05),尿中PD排出量随进食水平提高而增加(P<0.05),而尿中肌酸酐的排出量没有明显差异(P>0.05),尿中PD排出量可以用来估测瘤胃微生物蛋白质产生量。     

小分子植物病毒抑制物质研究进展

论述了小分子病毒抑制物的结构及对病毒的抑制机理 ,讨论了植物病毒抑制剂研究中存在的问题及研究前景     

肉鸽营养需要与饲料研究进展

概述了近年来我国肉鸽的能量、蛋白质等营养需要与饲料研究进展，提出了建立攻关协作网络和研究、制定、颁布肉鸽饲养标准的建议，期望对肉鸽的科研、生产及发展有所帮助。     

Model Establishment for Simulating Soil Organic Carbon Dynamics

Assuming that decomposition of organic matter in soils follows the first-order kinetics reaction,a computer model was developed to simulate soil organic matter dynamics. Organic matter in soils is divided up into two parts that include incorporated organic carbon from crop residues or other organic fertilizer and soil intrinsic carbon. The incorporated organic carbon was assumed to consist of two components, labile-C and resistant-C. The model was represented by a differential equation of dCi/dt = Ki× fT × fw × fs × Ci ( i = l,r, S ) and an integral equation of Cit = Cio × EXP ( Ki X fT X fw X fs X t ). Effect of soil parameters of temperature, moisture and texture on the decomposition was functioned by the fT, fw and fs, respectively.Data from laboratory incubation experiments were used to determine the first-order decay rate Ki and the fraction of labile-C of crop residues by employing a nonlinear method. The values of K for the components of labile-C and resistant-C and the soil intrinsic carbon were evaluated to be 0. 025,0. 080 × 10-2 and 0. 065 ×10-3d-1, respectively. The labile-C fraction of wheat straw, wheat roots, rice straw and rice roots were0.50, 0.25, 0.40 and 0.20, respectively. These values are related to the initial residue carbon-to-nitrogen ratio ( C/N) and lignin content.     

环太湖典型丘陵区不同土地利用下土壤磷素随地表径流迁移特征

在环太湖丘陵地区选择4种有代表性的土地利用类型进行野外原位试验，研究了自然降雨条件下磷素随地表径流的迁移特征。结果表明，各场降雨水相总磷的“次降雨径流平均浓度”呈对数正态分布。悬浮颗粒态磷是水相磷素迁移的主要形态，占水相总磷的76%～89%，其浓度主要由表土总磷含量及地表径流量决定;而溶解态磷（DP）主要与表土中的有效磷含量以及径流与表土相互作用深度有关。溶解态无机磷（DIP）占溶解态磷的57%～85%，竹林的DIP浓度最高。菜地径流的有机磷含量（DOP）明显高于另外3种土地利用。不同土地利用下溶解态和悬浮态磷的迁移通量分别为23～92.7mg·m^-2·a^-1和113.4～364.3mg·m^-2·a^-1，与地表径流量表现出较强的相关性。