大学图书馆网站建设的思考——以塔里木大学图书馆网站为例

本文分析了国内大学图书馆网站建设的环境及其重要性,总结了大学图书馆网站的特点与功能,提出了大学图书馆网站建设的设计思路。     

核桃叶片基因组DNA的4种提取方法比较

以核桃叶片为材料,比较了高盐低pH法、SDS法、改良CTAB法和适合酚类、多糖类物质较多的植物DNA提取法提取基因组DNA的效果;通过琼脂糖凝胶电泳、紫外分光光度计、RAPD、ISSR等方法进行PCR扩增和用EcoRI、PstI内切酶酶切对所提取的DNA样品进行检测,比较所得DNA的产量和质量.结果表明,4种提取方法从叶片中得到的DNA产量和质量有所不同,其中酚类、多糖类物质较多的植物DNA提取法产量最高,纯度适中.4种方法提取的DNA对以后的PCR和酶切均没有影响.     

杨树与溃疡病菌相互作用的生理病理化学研究——Ⅱ溃疡病菌毒素粗提物对寄主的影响初探

从含杨树树皮浸提液的改良Ｆｒｉｅｓ３号培养基培养的杨树溃疡病菌（Ｄｏｔｈｉｏｒｅｌａｇｒｅｇａｒｉａ）代谢产物中分离出毒素粗提物。对不同抗病性杨树材料处理结果发现，该粗提物能使叶子出现黑斑和萎蔫，并使材料细胞膜透性增大、脯氨酸含量增加，且对感病品种北京杨（Ｐｏｐｕｌｕｓ×ｂｅｉｊｉｎｇｅｎｓｉｓ）的影响大于抗病品种毛白杨（Ｐ．ｔｏｍｅｎｔｏｓａ）。这表明，溃疡病菌在活体外培养的代谢产物中含有毒素，初步定为８０％丙酮沉淀物     

Establishment of Plant Acceptor System for Hyperosmosis Transformation.

Calli and adventitious buds induced from different explants such as young stems and leaves of tomato variety Moneymaker,and mature embryos and young spikelets of rice variety TP309 were used to establish hyperosmotic transformation system at various osmotica treatments.The results revealed that the calli induced from tomato young leaves and rice young spikelets were the ideal transfomation acceptor.The cells fo calli were still vigorous when treated with 0.75 mol/L hyperosmotic solution for 4 hours.The differentiation rates of calli varied from 7.5% to 93.3% in different media.The bud differentiation was apparently inhibited by hyperosmotic treatments.0.75mol/L sucrose hypertonic solution and 0.2mol/L CaCl2 solution were favorable hyperosmoticum and hypoosmoticum respectively.     

Establishment and Identification of Cytoplasmic Male Sterility in Brassica napus by Intergeneric Somatic Hybridization

Exploitation of novel cytoplasmic male sterility(CMS)is a main approach for widening the cytoplasmic genetic background of hybrid oilseed rape and avoiding epidemic risk in oilseed rape production.In this study,symmetric somatic hybrids between Brassicanapus var.Zhongshuang4 and Sinapis arvensis(Yeyou18)were produced by protoplast fusion.Two of the six established hybrids were male sterile showing trace or no pollen release upon flowering with non-or slightly extended stamens.Using Zhongshuang4 as a recurrent parent to pollinate the male sterile plants,the ratio of male sterile plants increased with the number of backcrosses.As early as in BC3 generation,most of the sterile families had nearly 100%sterile plants.Up to BC4 generation,the male sterility became stable and no fertility segregation was observed.All F1 progenies from tested crosses using restorer and maintainer lines of Polima CMS were 100%sterile,indicating that the established CMS by somatic hybridization is different from Polima CMS.The origin of the cytoplasm and potential use of this hovel CMS in oilseed rape breeding were discussed.Key wotds:Oilseed rape,Protoplast fusion,Cytoplasmic male sterility,Sinapis arvensis     

板粟插穗愈伤组织及不定根的细胞组织学研究

板栗扦插不易生根,用HL—43生根剂处理扦插板栗嫩枝,进行愈伤组织及不定根形成的细胞组织学研究,结果证明:其插穗愈伤组织及不定根的形成,需经过诱导起动,分裂分化及器官形成三个阶段。不定根的形成可以产生在插入苗床的枝段各个部位,以基部1—3节最多,主要由维管形成层细胞或韧皮部薄壁细胞分裂产生。愈伤组织只在剪口处发生,并停留在此阶段,仅有少数愈伤组织形成不定根。     

国外图书馆先进经验与我国图书馆服务的可持续性发展

分析了国外图书馆在服务领域内的各种先进经验，针对我国图书馆服务工作的现状，提出了深化与可持续性发展的各项措施：开展我国图书馆工作中的特殊群体服务；实现图书馆联网，开展馆际互借；开展各种特色服务，拓展服务范围；咨询人员专业化，全面开展咨询工作,尤其是数字参考咨询服务，提升我国图书馆的咨询服务水平。

     

麦黑斑潜叶蝇幼虫空间分布及抽样技术研究

研究结果表明 :麦黑斑潜叶蝇幼虫在小麦田属于聚集分布 ,分布的基本成分是个体群 ,聚集度随着种群密度升高而增大 ;幼虫在不同虫口密度下最适抽样公式为 :N =t2 ( 2 .90 3 3 /m +0 .3 2 73 ) /D2 ;有虫株率与平均虫口密度关系式为 :m =-0 .0 62 6+0 .0 3 67p(r=0 .992 4 )。     

Linearly polarized emission from colloidal semiconductor quantum rods

Colloidal quantum rods of cadmium selenide (CdSe) exhibit linearly polarized emission. Empirical pseudopotential calculations predict that slightly elongated CdSe nanocrystals have polarized emission along the long axis, unlike spherical dots, which emit plane-polarized light. Single-molecule luminescence spectroscopy measurements on CdSe quantum rods with an aspect ratio between 1 and 30 confirm a sharp transition from nonpolarized to purely linearly polarized emission at an aspect ratio of 2. Linearly polarized luminescent chromophores are highly desirable in a variety of applications.