播期密度对不同小麦品种群体茎数及产量的影响

参照四水平五因素正交L16(45)设计,研究品种、播期与密度对小麦总茎数和产量及产量构成因素的影响。结果表明,冬前,密度对小麦基本苗影响最大,播期对分蘖数和总茎数影响最大;春季,品种对单株茎数、总茎数和成穗数影响最大。品种对产量三要素影响显著,播期对穗数和千粒质量影响显著,对穗粒数影响不显著,密度对千粒质量影响显著,对穗数和穗粒数影响均不显著。播期对小麦产量影响最大,其次为品种,密度影响最小。适宜的播期在10月4日至10月10日,随着播期后移,小麦产量明显下降,中麦895是本试验中获得高产的最优品种。合理的种植密度为210万～270万/hm2,小麦处于最佳品种、播期、密度组合时,小麦冬前、春季群体总茎数最多,小麦获得最高产,产量达9 000kg/hm2。     

森林资源管理与监测地理信息系统的功能模块设计

介绍了一种新的森林资源数据和图面资料的数字化、信息化管理方法。系统以35技术为支撑，可以实现小班数据和林相图的同步更新等多种功能，从而避免了传统森林资源管理方法中存在的一些问题，使得森林资源信息管理更加符合现代化发展的需要。     

Construction of recombinant adenovirus expressing BDNF and its expression in expanded rat mesenchymal stem cells in vitro

AIM:To construct recombinant adenovirus vector containing brain derived neurotrophic factor, (BDNF) gene using bacterial homogenous recombination, and investigate the expression in expanded rat mesenchymal stem cells (rMSC) in vitro.METHODS:BDNF gene and proBDNF gene were subcloned into adenovirus shuttle plasmid pAdTrack-CMV containing enhanced green fluorescent protein gene (EGFP) expression cassette, forming shuttle vector of pAdTrack-BDNF, and pAdTrack-proBDNF, and co-transformed into BJ5183 bacterial cells with adenovirus backbone vector pAdEasy-1 using chemical transformation. After the recombinant adenovirus vector was obtained, the identified recombinant adenovirus plasmid DNA was digested with Pac I and transfected to 293 cells to package recombinant adenovirus particles. rMSC were infected by recombinant adenovirus and EGFP expression was detected using fluorescent microscope. Infection efficiency was assessed by flow cytometrics. Western blotting identified expression of Ad -proBDNF and Ad-BDNF in rMSC. rMSC infected with Ad -proBDNF and Ad-BDNF were induced to differentiate into neuron-like cells. rMSC infected with Ad -proBDNF and Ad-BDNF were injected into nude mice and assessd in vivo.RESULTS:We successfully constructed the recombinant adenovirus Ad -proBDNF and Ad-BDNF that expressed in expanded rMSC in vitro.CONCLUSION:Recombinant adenovirus high-effectively mediates Ad -proBDNF and Ad-BDNF expression in expanded rMSC in vitro and in vivo.     

中国吉林省和韩国野生大豆的遗传多样性及遗传关系分析

利用9对多样性较高的SSR引物分析了来自中国吉林省(36份)和韩国(40份)野生大豆材料的遗传多样性,结果表明:在全部76份材料中,共检测到172个等位基因,每对引物平均获得19.1个.其中,在韩国的野生大豆资源中,每对引物检测到等位基因数11-18个,平均13.7个.中国吉林省的野生大豆资源中,每对引物检测到等位基因数5～16个,平均12.3个.不论中国吉林省还是韩同的野生大豆都具有较高的多态性信息含世(PIC),分别为0.821和0.868,两者遗传多样性并没有明显差异,聚类分析结果表明可将中国吉林省与韩闻的野生大豆分为两大类群.     

中国动物保健品现状、问题及对策之探讨——由欧盟对我国动物产品残留检查引发的思考

欧盟于 1 999年 1 0月就进口我国动物产品再次来我国进行检查 ,主要目的是调查我国养殖业动物用药及动物源食品的药物残留问题。本文借此对我国动物药品使用管理等方面的现状、问题及对策加以探讨。     

黑斑口虾蛄人工育苗技术研究

通过黑斑口虾蛄人工育苗试验。初步研究其生态习性、繁殖重演学及其幼体发育的特性。认为水温、环境稳定性及光周期是影响其抱卵孵化成功与否的主要因素,水温、光照、底质或人工洞穴、饵料是影响幼体谱态率及成活率高低的关键因子。黑斑口虾蛄的伪蚤状幼体生长过程可分为３个阶段：卵黄营养阶段、浮游变态率及成活率高低的关键因子。黑斑口虾蛄的伪蚤状幼体生长过程可分为３个阶段：卵黄营养阶段、浮游阶段及底栖阶段,仔虾蛄比底栖     

Aluminum tolerance associated with enhancement of plasma membrane H+-ATPase in the root apex of soybean

Seventeen soybean cultivars were screened to discern differences in aluminum (Al) sensitivity. The Sowon (Al-tolerant) and Poongsan (Al-sensitive) cultivars were selected for further study by simple growth measurement. Aluminum-induced root growth inhibition was significantly higher in the Poongsan cultivar than in the Sowon cultivar, although the differences depended on the Al concentration (0, 25, 50, 75 or 100 μmol L^–1) and the amount of exposure (0, 3, 6, 12 or 24 h). Damage occurred preferentially in the root apex. High-sensitivity growth measurements using India ink implicated the central elongation zone located 2–3 mm from the root apex. The Al content was lower 0–5 mm from the root apices in the Sowon cultivar than in the apices of the Poongsan cultivar when exposed to 50 μmol L^–1 Al for 12 h. Furthermore, the citric acid exudation rate was more than twofold higher in the Sowon cultivar. Protein production of plasma membrane (PM) H⁺-ATPase from the root apices (0–5 mm) was upregulated in the presence of Al for 24 h in both cultivars. This activity, however, decreased in both cultivars treated with Al and the Poongsan cultivar was more severely affected. We propose that Al-induced growth inhibition is correlated with changes in PM H⁺-ATPase activity, which is linked to the exudation of citric acid in the root apex.     

产磷酸吡哆醛转移酶微生物菌株的筛选

筛选产磷酸吡哆醛转移酶微生物菌株,应用微生物产生的磷酸吡哆醛转移酶转化生产磷酸吡哆醛,以焦磷酸为磷酸基团供体磷酸化吡哆醛合成磷酸吡哆醛,采用HPLC法检测反应液,紫外吸收波长388nm,出峰时间为1.75min。得到1株转化效率较高的菌株C2-300,其转化效率达到64μg/mL。     

2种家养动物朊蛋白基因的扩增与分析

采用PCR方法扩增了云南矮马和北京油鸡的PrP^C基因，并进行序列测定，结合已发表种属朊病毒基因序列，运用分子生物学软件进行同源性分析。结果表明，云南矮马PrP^C基因与已报道的马PrP^C基因比较，其同源性达99％，氨基酸同源性达100％。与牛PrP^C基因比较，同源性达89％，氨基酸同源性达91％。北京油鸡PrP^C基因与鸡的已知序列比较，同源性达99％；与鸭、鸽、鹌鹑的已知序列比较，同源性94％～97％。其翻译的氨基酸序列与鸡的已知序列比较，同源性达99％，与鸭、鸽、鹌鹑比较，氨基酸同源性88％～99％。从本试验结果来看，云南矮马PrP^C基因与牛的PrP^C基因同源性较远，因此感染牛源朊病毒的风险较小，禽类与哺乳动物PrP^C氨基酸属于2个不同的进化分支，因此哺乳动物海绵状脑病不易传染给禽类或引起朊蛋白构型上的改变。这些结果可为海绵状脑病种间屏障补充详细的数据，也为制定相关预防控制措施提供了一些理论依据。     

温度、光照与盐度对雨生红球藻诱变株虾青素累积的调控

以MAV培养基为基本培养基，以雨生红球藻（Haematococcus pluvialis）诱变株1号为研究材料，进行物理因子对雨生红球藻虾青素累积的调控实验。温度梯度为15℃、20℃、25℃、30℃和35℃；光照梯度为黑暗、1000lx、2000lx、4000lx．6000lx和8000lx；盐度设0、4、8、12、16和20。进行单因子的胁迫实验，测定各组的虾青素含量和藻密度，结果显示，雨生红球藻生长的最适条件为温度15-20℃，光照1000-2000lx，盐度0。虾青素累积量最高时的温度为25～30℃，光照为8000lx，盐度为4～8。进一步进行盐度、光照的两因素胁迫正交实验，并检验两者的交互作用。其中盐度设4、8、12三个水平，光照强度设6000lx、8000lx、10000lx三个水平。结果光照为8000lx、盐度为8时获得最高的虾青素含量，其交互作用非常显著。