不同春小麦品种耐低磷性评价及种质筛选

筛选磷高效作物是充分利用土壤磷素和减少磷肥施用量的重要手段。本研究以162份春小麦种质资源为材料,对其苗期的株高、总根长、根表面积等8个指标的耐低磷系数进行分析,采用隶属函数法综合评价春小麦苗期的耐低磷特性,初步筛选耐低磷材料,并进一步进行成株期的耐低磷特性鉴定,筛选出耐低磷材料和磷敏感材料,分析其在低磷下酸性磷酸酶的活性变化。结果表明,低磷胁迫下春小麦材料苗期和成株期的各性状均受到不同程度的影响,并随着胁迫程度的增加,小麦生长受抑制程度增强。通过主成分分析将苗期8个指标转化成4个综合指标(累计贡献率为82.60%),将成株期的10个指标转化为3个综合指标(累计贡献率为83.23%);采用隶属函数法计算耐低磷综合评价值(D)值,对D值进行聚类分析,将苗期的162份春小麦种质资源划分为耐低磷型(10份)、较耐低磷型(26份)、低磷较敏感型(91份)、低磷敏感型(35份)4类。选取耐低磷型材料(5份)和低磷敏感型材料(4份),进一步进行成株期鉴定,最终筛选1份耐低磷材料wp-35和1份磷敏感材料wp-119。通过分析其酸性磷酸酶活性,发现在低磷胁迫下春小麦根系和叶片中的酸性磷酸酶活性均升高,且耐低磷材料的酸性磷酸酶活性高于磷敏感材料。本研究结果可为解析春小麦耐低磷特性、培育耐低磷品种提供种质资源和理论依据。     

辅导员在高职“后进生”转化中心理效应的运用

高职“后进生”不良行为转化一般要经历醒悟、转变、反复、稳定等几个过程,辅导员在不同的阶段运用不同的心理效应可以帮助高职“后进生”更好地转化,矫正其不良行为.     

一种改进的开关磁阻电机磁链模型

开关磁阻电机(SRM)特殊的结构使建立其磁链模型比较困难。根据SRM运行的周期特点,从数学角度用傅里叶级数模型对其进行解析,通过权系数将傅里叶级数模型与传统指数模型结合,得到一个新的改进模型。用提出的改进模型与指数函数模型、傅里叶级数模型分别对Ansoft Maxwell仿真得到的磁链数据进行拟合。对比解析结果,证明提出的改进模型具有更高的解析精度,适合解析磁链。     

基于EXCEL的作物灌水率图自动绘制与修正

在灌区的规划设计中,传统作物灌水率图的绘制一般都是手工方法,不仅效率低且修正起来很不方便,而且现有的灌水率图绘制软件一般存在着灌水率计算和灌水率图绘制相互独立、无法实时动态修正等不足.基此,综合利用EXCEL软件强大的数据处理、分析功能以及图形自动绘制功能,将灌水率计算与灌水率图绘制有机结合,从而实现灌水率图的快速绘制和动态调整.这种方法操作方便、简单,值得在工程设计部门推广使用.     

响应面优化丁烷-亚临界法制取桐油技术的研究

桐油是一种重要用途的干性油脂,不同工艺会对油品质量产生重要影响。为了克服传统工艺在制油中的缺陷,本研究采用丁烷-亚临界法工艺进行桐油的制备,并基于Design-Expert软件进行响应面优化实验,获得最佳工艺参数:萃取温度40.64℃,萃取时间45.38 min,原料颗粒度39.71目,在此条件下桐油得油率达到66.12%。采用气质联用仪分析了桐油脂肪酸组成为α-桐酸74.93%,亚油酸8.62%,油酸7.12%,棕榈酸2.72%,硬脂酸3.85%,通过分析所得油样的5项指标,可知该工艺所得油品满足国标要求。     

Study on Developmental Expression of ApoCⅡ Gene mRNA in Liver Tissues of Pigs

The aim of this study was to investigate the developmental patterns of ApoCⅡ gene mRNA in liver in Mashen and Large White pigs, and study the relationship between the expression level of ApoCⅡ and the lipid metabolism in pigs.The mRNA relative expressions of ApoCⅡ gene in liver at seven stages of 1,30,60,90,120,150,and 180-day old in Mashen and Large White pigs were determined by quantitative Real-time PCR.The results showed that the developmental trend of ApoCⅡ mRNA expression in liver between Mashen and Large White pigs was different.The ApoCⅡ mRNA abundance was decreased from birth to 60-day old,then increased at 90-day old,and decreased again after that in Mashen pig.However,the relative expression amount in Large White pig was gradually decreased from birth to 150-day old and increased again at 180-day old.Except for the ApoCⅡ mRNA expression amount at 1-day old,the differences of the expression amount at other stages in Mashen and Large White pigs were significant or extremely significant (P<0.05 or P<0.01).The ApoCⅡ mRNA expression in liver was affected by age and breed,and could play an important role in lipid metabolism in pigs.     

感应式高压静电喷头雾滴荷电效果影响因素分析

为研究如何在有限的充电电压下获得良好的雾滴荷电效果,首先建立了雾滴荷电过程的等效模型,对影响雾滴荷电效果的关键性参数进行理论推导;然后采取圆环形和仿形两种电极形式,取3个高压电极的关键性技术参数,设计4种不同的高压电极方案;最后配合2、2.5mm两种不同的绝缘层厚度,为雾锥角为80°的空心圆锥雾喷头TR 80-04设计了8种不同的高压静电罩,用网状目标法与法拉第筒法结合的荷质比测量系统对其荷电比进行测量,以荷质比评价其荷电效果的优劣。结果证明:理论分析与试验研究达到了良好的一致性,仿形电极的荷电效果明显优于圆环形电极,且电极宽度、电极中心到喷口的轴向距离与雾滴荷质比正相关,为高压静电罩的合理设计提供了可靠的理论和试验依据。     

葡萄霜霉病菌侵染抗病和感病品种过程的组织学观察

用苯胺蓝染色结合扫描电镜的方法,观察葡萄霜霉病菌(Plasmopara viticola)在葡萄感病品种‘红提’和抗病品种‘北玫’中的侵染过程,发现两品种从接菌到显症的过程基本一致,但孢子囊伸出气孔的时间、伸出孢囊梗气孔比例、孢囊梗数量及孢囊梗分支,‘红提’比‘北玫’多;菌丝在‘北玫’上生长速度慢,后期生长量也较少。‘红提’的潜育期为4 d,而‘北玫’的为5 d,‘红提’和‘北玫’的长菌丝、分支的长菌丝、疏松菌丝体出现的时间也不同。菌丝生长一段时间后,在‘北玫’上停留在疏松菌丝体阶段,而‘红提’96 h时叶片组织内长满致密的菌丝体。两品种在初生菌丝和长菌丝阶段产生吸器的频率差异显著。     

Preparation of Adjuvant Diluent for Live Vaccine against Classical Swine Fever and Evaluation of its Immune Effects

The aim of this study was to develop a novel oil-in-water (O/W) emulsion as adjuvant diluents (AD) for live vaccine against classical swine fever (CSF) that could effectively enhance the immune effect of vaccine.The AD was prepared by high-pressure homogenization technique.Formulations and preparation parameters were optimized with response surface design.Its stability, particle size, polydispersity (PDI) and Zeta potential were characterized.The humoral immune response and cellular immune response of the AD were evaluated with BALB/c mice by intramuscular injection.The particle size of the AD prepared by optimized formulation and parameters was 100.4 nm, PDI was 0.147, and Zeta potential was —28.7 mV.The experiment results showed that the AD had good stability.The AD was inoculated combined with live vaccine against CSF into BALB/c mice by intramuscular injection.The results showed that the live vaccine against CSF specific immune responses could be evoked in mice by co-inoculation with AD and vaccine.The cellular immune response levels in co-inoculated groups were significantly higher than control group (P<0.05), with obvious phenomena of higher levels of IFN-γ, IL-6 and IL-4 in serum.The result revealed that cellular immune capability significantly improved with the AD.The results strongly revealed that cellular immune capability significantly improved by introducing AD for effective immune-adjuvant for live vaccine against CSF.