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21.
不同叶龄蘖、穗氮肥组合对粳稻产量及氮素利用的影响 总被引:4,自引:0,他引:4
WANG Yan YI Jun GAO Ji-Ping ZHANG Li-Na YANG Ji-Fen ZHAO Yan-Ze XIN Wei ZHEN Xiao-Xi ZHANG Wen-Zhong 《作物学报》2020,46(1):102-116
以主茎叶片数不同的粳稻品种吉粳88 (14片)、沈农265 (15片)和沈农1401 (16片)为试材,采用大田筒栽方式,在总施氮量225kghm–2及轻简施肥(基肥、蘖肥、穗肥)模式基础上,设置基蘖肥∶穗肥6∶4和8∶2两种施肥比例,并分设不同源、库叶龄期施氮组合即不同叶龄蘖、穗肥精确施氮组合。分析了不同源库期氮肥运筹模式对水稻农艺性状、产量及氮素利用特性的影响。结果表明:(1)在有效穗数、分化颖花数、产量和氮素利用率方面,吉粳88、沈农265、沈农1401不同氮肥运筹下最佳蘖、穗肥叶龄组合均为6∶4显著高于8∶2。(2)不同氮肥运筹下,吉粳88在8叶(叶龄指数57.1%)、沈农265在9叶(叶龄指数60.0%)、沈农1401在10叶(叶龄指数62.5%)时,即叶龄指数在60%左右时,施用蘖肥效果最佳,最终穗数最多,对保蘖起主要作用;吉粳88在11叶(叶龄指数78.6%)、沈农265在12叶(叶龄指数80.0%)、沈农1401在13叶(叶龄指数81.3%)时,即叶龄指数在80%左右时,施用穗肥效果最佳,最终穗粒数最多,对促花起主要作用。(3)吉粳88-6∶4 (8, 11),沈农265-6∶4 (9, 12),沈农1401-6∶4 (10, 13) 3组处理,在产量、氮素积累量、氮素吸收利用率、农学利用率及偏生产力等方面,显著高于同品种不同叶龄蘖、穗氮肥组合中的其他处理。因此,适当延迟蘖肥施用叶龄期(叶龄指数60%左右)、提前穗肥施用叶龄期(叶龄指数80%左右)同时增加穗肥施用比例,既可以显著提高氮素积累量、氮素吸收利用率、农学利用率及偏生产力,又能显著促进成穗率的提高和颖花数的分化,达到保蘖促花的双重作用,实现优源、扩库、充实的目标,从而获得高产。 相似文献
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用宏基因组学方法研究绿肥对水稻根际微生物磷循环功能基因的影响 总被引:1,自引:0,他引:1
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异色瓢虫成虫对豌豆修尾蚜的捕食作用 总被引:1,自引:0,他引:1
初步研究了异色瓢虫(Harmonia axyridis Pallas)的雌、雄成虫对豌豆修尾蚜(Megoura japonica Matsumura)的捕食作用,采用Holling-Ⅱ型方程功能反应模型对异色瓢虫雌、雄成虫捕食豌豆修尾蚜的效果进行拟合。结果表明,异色瓢虫雌、雄成虫的功能反应均符合Holling-Ⅱ模型。而在一定空间和相同比例猎物条件下,种内干扰会对异色瓢虫成虫捕食豌豆修尾蚜产生影响,此试验中干扰系数为1.09。 相似文献
25.
不同产地红茶主要品质成分分析 总被引:3,自引:0,他引:3
通过收集国内外不同地区具有代表性的红茶茶样,对其进行感官审评与理化成分分析。结果表明,感官方面,国外红茶样整体水平优于国内红茶样品。内含品质成分方面,云南大叶种红茶水浸出物含量较高;氨基酸含量则均在3%~5%之间,差异并不明显;国外红茶样茶多酚平均含量高出国内红茶样2.66%;咖啡碱含量在2.35%~5.45%之间,其中产于斯里兰卡的两个红茶样高于其他地区红茶;国外红茶样茶黄素含量整体较高,茶红素以肯尼亚红碎茶含量最高,国内红茶样则是茶褐素含量普遍较高。 相似文献
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AIM: In order to observe the myocardial differentiation capacity of the dedifferentiated fat (DFAT) cells treated with vitamin C in vitro. METHODS: DFAT cells were dedifferentiated from the mature rat adipocytes with ceiling adherent culture. The DFAT cells of passage 3 were used in the study. Vitamin C and/or neonatal rat heart tissue lysate were added into the culture medium to induce myocardial differentiation for 3 weeks. The cell morphology was observed under microscope. The myocardial-specific markers, such as cTnT, GATA-4 and NKx2.5, were examined by the methods of immunofluorescence, PCR and Western blot. RESULTS: Mature rat adipocytes dedifferentiated into fibroblast-like DFAT cells after ceiling adherent culture. The DFAT cells spontaneously differentiated into cardiomyocyte-like cells under normal culture condition with a low incidence. After treated with neonatal rat heart cell lysate, the DFAT cells became cardiomyocyte-like cells that had bigger size, longer shape and myotubule-structure. The expression of cTnT, GATA-4 and NKx2.5 was remarkably increased at both mRNA and protein levels as compared with the normal cultured DFAT cells. The expression of cTnT, GATA-4 and NKx2.5 was further increased in DFAT cells after treating with vitamin C. No spontaneous beating cell was observed. CONCLUSION: Vitamin C enhances the differentiation of DFAT cells into cardiomyocyte-like cells. 相似文献
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By the infection of Brucella virulent strain and attenuated strain in mice macrophage RAW264.7,the assay was aimed to explore the relationship between NF-κB signaling pathways and Brucella virulent strain and attenuated strain in intracellular survival.Use different MOI Brucella (2308,RB51,16M and M5) to infect mice macrophage RAW264.7,after 0,4,8 and 24 h infected,cracking cell and collecting supernatant,we detected the effect of Brucella on activation of NF-κB signaling pathway by Western blotting.Different concentrations of NF-κB signaling pathway inhibitor were incubated with mice macrophage RAW264.7,with different multiplicities of infection (MOI) of Brucella infecting cells,ELISA kits to detect the expressions of TNF-α,IL-1β and IL-6 cytokine;At the same time,count the number of intracellular bacteria of CFU.The results showed that rough cattle Brucella strains RB51 could strongly activate NF-κB signaling pathway,smooth cattle Brucella strains 2308 was weak in the activation;At the same time,the activation of NF-κB signaling pathway was concentration dependent.When the MOI was 80,infection time was 8 h,NF-κB activation degrees of rough cattle Brucella strains RB51 and smooth cattle Brucella strains 2308 were the strongest,and this pathway was involved in producing TNF-α and IL-6;NF-κB signaling pathway inhibitor BAY11-7082 affected Brucella intracellular survival.So rough cattle Brucella strains RB51 intracellular survival and NF-κB signaling pathway activity were closely related.The results laid the foundation for the further study of Brucella intracellular pathogenesis,also provided scientific basis for the research of new drugs to Brucella,and prevention and treatment of brucellosis. 相似文献
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