卧龙湖省级自然保护区建设规划

通过对卧龙湖自然保护区现状和卧龙湖干涸原因进行分析,探讨卧龙湖自然保护区生态修复规划,旨在为卧龙湖自然保护区整体建设提供科学依据和参考。为沈阳市康平县地区经济和社会可持续发展创造良好环境。     

A study on the mechanism of doxorubicin promoting IL-1β and collagen type I secretion in cardiac fibroblasts

AIM To observe the effect of adriamycin/doxorubicin （DOX） on the production of inflammatory cytokines and collagen in cardiac fibroblasts and its mechanism. METHODS Neonatal SD rat cardiac fibroblasts were isolated， cultured， and identified by immunofluorescence staining with monoclonal antibodies against vimentin observed under a confocal laser-scanning microscope. The Cell Counting Kit-8 assay was used to detect the toxicity of DOX on cardiac fibroblasts， and flow cytometry with annexin V-FITC/PI double staining was used to detect apoptosis. ELISA was used to detect the release of inflammatory factors in the supernatant of cultured cells. Immunofluorescence labeling assay was used to detected α-smooth muscle actin （α-SMA） expression and mitochondrial reactive oxygen species （mROS） in the cells. Western blot was used to detect the expression of nucleotide-binding oligomerization domain-like receptor protein 3 （NLRP3） inflammasome-related proteins in cardiac fibroblasts. RESULTS （1） Compared with the control group， DOX inhibited the proliferation of cardiac fibroblasts （P<0.05）， but had no significant effect on apoptosis （P>0.05）. （2） Treatment with DOX promotes the release of proinflammatory factors interleukin-1β （IL-1β） and IL-6 in cardiac fibroblasts （P<0.05）. （3） The expression of α-SMA， collagen type I and transforming growth factor-β in DOX treatment group increased significantly compared with control group （P<0.05）. （4） Compared with the control group， the levels of mROS， cellular NLRP3 and cleaved caspase-1 in cardiac fibroblasts increased significantly after DOX treatment. CONCLUSION Doxorubicin promotes cardiac fibroblasts to secrete IL-1β and collagen type I by promoting mROS production and activating NLRP3 inflammasome.     

动物生态营养学在崛起

传统的动物营养学用静态的思维、机械的方法和孤立割裂的方式来研究复杂机体的物质和能量代谢的特性,只重视单纯的物理化学特性,忽略家畜整体功能及其与环境的相互作用.动物生态营养学以生态学和生态经济学理论为基础,应用系统科学的思维原则和研究方法,把环境-动物-产品作为一个整体,研究动物在多维环境条件下,对各营养要素的动态需求量及其相互关系,在精确数量化的基础上,用计算机模拟物质、能量流的动态转化、平衡及其调控模式,以求达到用尽可能少的饲料(草)资源,在尽可能短的周期内,生产出尽可能多的优质畜产品,获得尽可能大的经济效益,达到(或维持)尽可能最佳的生态平衡.     

东方红-YZDZ80C液压自行式移动电站

介绍东方红-YZDZ80C液压自行式移动电站的主要技术参数和主要部件结构性能特点。     

微卫星DNA标记在家兔遗传育种中的应用

微卫星DNA是一类由1～6个核苷酸为重复单元而形成的串联重复序列,由于它具有含量丰富、多态性高、共显性等优点.而广泛应用于鉴定亲缘关系、构建遗传图谱、数量性状位点(QTL)定位、遗传多样性估计、标记辅助选择等方面.本文就微卫星标记在家兔遗传育种中的应用进行概述.     

红芪愈伤组织中黄酮代谢途径的探讨

在红氏供试材料中苯丙氨酸转氨酶活性在分化愈伤组织中最高，但游离苯氨丙酸含量在分化愈伤组织中并无明显降低，说明游离氨基酸的积累主要来自蛋白质的分解。黄酮含量的变化以褐化愈伤组织中含量最高，因此我们推测：黄酮合成的最后几步，可能发生在褐化愈伤组织中，从可溶性蛋白质的ＳＤＳ－ＰＡＧＥ平板电泳结果表明，分化愈伤组织与褐化愈伤组织相比蛋白质谱带发生了明显的变化，这可能与控制黄酮合成的有关酶的活性变化有关。     

Antitumor effect of heat-killed lactobacilli adhered to cervical carcinoma cells

AIM: To observe the immune property of cervical carcinoma and antitumour effect of heat-killed lactobacilli adhered to HeLa cells. METHODS: Flow cytometry was used to analyze the expression of immune molecules on HeLa cells after adhered with heat-killed lactobacilli. MTT was used to analyze the cytotoxity of lymphocytes on cervical carcinoma cells. RESULTS: Adherence of heat-killed lactobacilli to HeLa cells did not affect the expression of MHC-I molecules (P>0.05), but enhanced the expression of CD80, CD86 significantly (P<0.05) in HeLa cells. Adherence of heat-killed lactobacilli to HeLa cells also enhanced the cytotoxity of NK cells. Compared to control, the cytotoxity of CTL to HeLa carcinoma cells was significantly enhanced by the adherence of heat-killed lactobacilli to HeLa cells (P<0.05). CONCLUSION: Heat-killed lactobacilli strengthen the antitumor effect on HeLa cells.     

基于C／S模式的网上书店管理系统设计

本文了给出基于C／S模式的网上书店管理系统的方案设计和数据库设计模式。     

河岸渗滤系统除污功效的研究进展

河岸渗滤系统对污水具有净化功能.介绍了河岸渗滤系统的除污功效,为实现城市污水资源化提供依据.     

图书馆复印机的作用与使用维护

介绍了复印机在现代图书馆工作中的重要作用,通过对复印机常见故障的分析,提出了解决方法,指出日常维护和保养可以保证复印机的完好工作状态,提高复印服务水平,延长使用寿命,达到节能减耗的目的.