三七NAC基因家族全基因组鉴定与表达分析

为明确三七Panax notoginseng NAC转录因子基因家族的分布、功能和结构,通过生物信息学分析法进行鉴定,对其理化特性、染色体位置和进化特征进行分析,并根据RNA-seq数据分析其家族成员的时空表达模式和受黑斑病菌Alternaria panax诱导后的表达情况。结果显示,三七中共有98个NAC基因家族成员,其编码蛋白质长度介于104～882个氨基酸之间,分子量在11.78～100.20 kD之间,等电点在4.12～9.75之间。这98个NAC基因家族不均匀地分布在三七的12条染色体上,其中1号染色体分布最多（16个）,而11号染色体上分布最少（1个）。三七NAC基因启动子区域存在与光响应、生长素响应、赤霉素响应及茉莉酸甲酯响应等相关的多种顺式作用元件。NAC基因在三七不同组织及根部不同发育时期均有表达,在受到黑斑病菌侵染的叶片中NAC部分基因家族成员显著上调表达。表明NAC基因家族在三七的生长发育和响应黑斑病菌侵染过程中具有重要作用。     

生物多样性与地形及土壤因素对坡面产流产沙的耦合影响

生物多样性关系到生态系统的功能,而坡度和土壤因素是影响坡面产流产沙的重要因子。本研究基于黄土丘陵区生物多样性,并引入坡度、降雨前土壤含水量和土壤容重,探究其与坡面产流产沙总量的耦合关系。通过野外人工降雨试验,记录草本、灌木和乔木群落下的产流时间和产流产沙总量,并开展土壤植被调查。结果表明,与草本和乔木群落相比,灌木群落在延长产流开始时间和降低产沙总量上表现更优;植被群落功能离散度指数(FDiv)对产沙总量影响最大,Simpson指数(D)对产流总量影响最大,坡度仍然是不可忽视的第二重要影响因素;产流总量与坡度、土壤容重、D和功能丰富度指数(FRic)的回归关系优于产流总量分别与其的回归关系;产沙总量与坡度和FDiv指数的回归关系优于产沙总量分别与其的回归关系。植被保护与坡度改善将是该区水土保持规划工作的重点。     

Wavelet Application in Algorithms of Data Mining

Wavelets have been widely applied in such research areas as image processing, computer vision, network management and data mining. Wavelets have many favorable properties, which are hierarchical and multiresolution decomposition structure, linear time and space complexity of the transformations. The paper presents wavelet application on the algorithms of data mining, in cluding as clustering, classification, distributed data mining, similarity search, approximate query processing, visualization and so on. Finally, the paper concludes by discussing the impact of wavelets on data mining research and outlining potential future research directions and applications.     

Stability Analysis on Excavated High Slope of Mozong River Power Station in Wulong

The subject matter of this paper is to analyze the stability of excavated high slope. On the base of typical engineering case in Wulong, Chongqing, the geological conditions and potential failure pattern of the high slope are discussed. Then, the limit equilibrium method and the stereographic projection method are used to synthetically analyze the stability of excavated high slope. As a result, it indicates that the stability of the high slope in insufficient and some retaining structures ought to be adopted for the high slope as soon as possible.     

Inspiration to Reuse of Old Industry Building with SOHO

This paper analyzes the SOHO and the reuse of old industry building, and deeply explores the creation and application of SOHO mode in the reuse of old industry building, furthermore, it points out the four inspirations to the reuse of old industry building, at the end, a general judgment of the development logic of SOHO is given.     

Empirical Analysis of the Demand of Rail Freight Transport in China on Basis of VAR Model

With the rapid development of road and other transport modes in China, the railway freight transport is confronted with great challenge. So, the relations of rail freight transport, development of national economy and extension of railway become focuses. This paper makes a cointegreting VAR model according to the data from Chinese Statistical Year Book. On the basis of the model of the long-run and short-run equilibrium of rail freight demand are analyzed, The result shows that the rail freight transport in China has a stable and low elastic coefficient of income and a high but unstable elastic coefficient of supply in long-run. In short dynamics, the national economy is the first factor affecting rail freight demand and in the second term the innovation reaches a top point. Extension of railway has little effect on rail freight transport. The innovations from rail freight transport and railway extension has little impulse on national economy. Rail freight is the important factor affecting extension of rail-way with a third-term top, but GDP has a little impulse on it. Further analysis on the result and suggestion on policy are made.     

CO2-O2混合喷吹对铁液中C、V元素氧化的影响

为了验证用CO2替代部分O2进行转炉氧化提钒的可行性,在实验室进行了CO2-O2混合喷吹提钒模拟实验。结果显示C的氧化量与C的氧化速率随CO2含量的增加而升高;V的氧化量随CO2含量的增加而降低,其中CO2含量为20%与纯O2对C、V的氧化量接近;纯O2喷吹时C的氧化量为34.56%,V的氧化量为96.85%;CO2含量为20%混合气体喷吹时C的氧化量为36.83%,V的氧化量为93.29%;以V的相对氧化量与C的相对氧化量的比值(ΔV/ΔC)来衡量提钒保碳能力,CO2含量为20%的最终ΔV/ΔC为5.96,CO2含量,40%、60%、80%的最终ΔV/ΔC均小于3.8;反应前期,V的氧化速率随CO2含量的增加而升高,反应后期V的氧化速率随CO2含量的增加而降低。     

11种兰属植物DNA的提取及RAPD-PCR实验体系的建立与优化

采用改进的CTAB-DNA提取方法，从11种兰属植物的嫩叶中提取总DNA。所得的DNA样品的A260／A280值在1．7～1．9之间，琼脂糖凝胶上主带清晰，较少降解，样品纯度高，DNA量大。另外，对影响RAPD-PCR的Mg^2＋、dNTPs、Taq酶、引物浓度等因素进行了优化。确定优化的反应体系为：75ng模板DNA，1×Buffer，2．5mmol／LMg^2＋，0．15mmol／LdNTPs，0．75UTaq酶，引物浓度0．4μmol／L，反应总体积为25山。该体系在20个供试兰属实验材料中获得较好的扩增结果。     

低能离子注入后小麦苗期损伤效应研究

以鉴54、豫农118及豫麦18号为材料,研究了不同剂量率、剂量离子注入对小麦幼苗生长的影响。结果表明,随着剂量率和剂量的增加小麦幼苗损伤程度逐渐加剧,具体表现在苗高降低、第一叶长变短。剂量率间差异显著,品种间有辐射敏感性差异。另外苗期还发现了一些变异,主要表现为主叶脉失绿和形态畸形。离子束诱变的合适剂量在6×1017ions/cm2以上。