绿洲区苜蓿与鸭茅混播饲草的青贮发酵品质研究

以苜蓿(Medicago sativa)与鸭茅(Dactylis glomerata)混播草地牧草为材料,按照90%苜蓿+10%鸭茅、70%苜蓿+30%鸭茅、50%苜蓿+50%鸭茅的比例进行青贮,通过分析对比发酵品质、营养成分、感官青贮效果等,以期优化最佳混合青贮的豆∶禾比例。试验结果表明:苜蓿和鸭茅混合青贮后,气味、质地、色泽感官评定均为优良,且pH呈下降趋势,青贮效果优于苜蓿和鸭茅的单独青贮。混合青贮降低了苜蓿青贮中氨态氮含量的36.4%,鸭茅青贮中乙酸、丙酸的含量明显下降;提高了苜蓿青贮中乳酸的含量,有效抑制了丁酸的产生,苜蓿+鸭茅饲草混贮比例为7∶3和5∶5处理结果未检测出丁酸。3个混合青贮组合的粗蛋白含量高出鸭茅单贮31.9%,酸性洗涤纤维低于鸭茅青贮12.6%。在青贮发酵过程中,苜蓿+鸭茅以7∶3比例混合青贮,有利于提高青贮饲料的发酵品质。     

不同类农作物秸秆在肉羊瘤胃中的降解特性研究

本试验旨在研究不同类农作物秸秆在肉羊瘤胃中的降解规律,以3头安装有永久性瘤胃瘘管的杜×寒肉用绵公羊为试验动物,以青贮玉米秸为对照材料,采用半体内尼龙袋法对不同产地的6类19个秸秆样品干物质(DM)和中性洗涤纤维(NDF)的降解率和降解参数进行测定。结果表明:秸秆DM和NDF的瘤胃降解规律相近,48 h后趋于稳定,72 h消失率和有效降解率(ED)最高的为藤蔓类的地瓜秧和花生秧,接近青贮玉米秸,玉米秸、大豆秸和稻草秸较低,小麦秸最低(P0.05);DM和NDF有效降解率与粗蛋白质(CP)、NDF和酸性洗涤纤维(ADF)的相关性较强(P0.01),拟合方程分别为DMD=0.9900+0.3270 CP-0.8099 NDF-0.1880 ADF(R~2=0.8860,P0.0001)、NDFD=0.5111+0.3833 CP-0.1519 NDF-0.3963 ADF(R~2=0.7585,P0.0001)。地瓜秧和花生秧在瘤胃中降解快,可作为肉羊较好的饲料来源,而玉米秸、大豆秸、稻草秸和小麦秸在瘤胃中降解效果较差。     

TNF-α基因在F18大肠杆菌抗性型和敏感型断奶仔猪间的差异表达分析

本研究运用Real-time PCR方法分析TNF-α基因在苏太猪F18大肠杆菌抗性型和敏感型断奶仔猪群体中11个组织中的表达谱,以及在抗性型和敏感型个体之间的差异表达,以探讨TNF-α基因在断奶仔猪抗F18大肠杆菌感染中的作用。结果表明:TNF-α基因在抗性型和敏感型个体所检测的11个组织中均有表达,其中在脾脏、肺、肾脏、胸腺和淋巴结组织中表达量较高,在十二指肠和空肠组织中呈中度表达;TNF-α基因在F18大肠杆菌抗性型个体各个组织中的表达量普遍高于敏感型个体,且脾脏、肾脏、胸腺和十二指肠组织中TNF-α基因在抗性型个体中的表达量显著高于敏感型个体(P0.05)。由此推测,TNF-α基因的较高表达可能有利于提升仔猪对F18大肠杆菌的抵抗能力,在F18大肠杆菌侵袭后引起的一系列免疫应答过程中发挥着重要的作用。     

鸡柔嫩艾美尔球虫病抗性主成分分析评估模型的建立

为建立鸡活体可测球虫病抗性综合评估模型,探索鸡球虫病抗性选育新方法,以京海黄鸡为试验材料,根据国内外文献选择15个球虫抗性评价指标,通过比较鸡柔嫩艾美尔球虫感染组和非感染组抗性指标的差异,进一步筛选活体可测指标,并进行主成分分析,通过各主成分和综合主成分与所有抗性指标的相关分析,筛选最优抗性综合评估模型,并进行模型有效性筛选。在所选的14个活体可测抗性指标中,有6个指标在感染和非感染组间存在显著或极显著的差异(P0.05或P0.01),他们分别是感染后第3—5天体增重、感染后第8天血浆中CAT、GSH-Px、MDA、SOD和IFN-γ的浓度;主成分分析建立了6个单主成分和累计贡献率大于80%的综合主成分的抗性评估模型,各抗性评估模型计算的综合选择指数与15个抗性指标相关分析的结果表明,第一主成分模型和7个抗性指标相关极显著(P0.01),2个相关显著(P0.05),特别是与盲肠病变记分相关极显著(P0.01),其余6个主成分评估模型只与15个抗性指标中的2~3个相关显著或极显著(P0.05或P0.01),且与盲肠病变记分相关均不显著(P0.05);经检验第一主成分模型选择指数值的分布为正偏态分布,排序后其大小与盲肠病变表现一致。因此,第一主成分模型fi1=-0.64Zxi1+0.31Zxi2+0.80Zxi3-0.05Zxi4-0.08Zxi5+0.59Zxi6可以作为鸡柔嫩艾美尔球虫抗性选择的最佳综合选择指数用于鸡的抗病育种。     

Phylogenetic analysis of Newcastle disease viruses isolated from wild birds in the Poyang Lake region of China

Newcastle disease virus (NDV) causes a highly contagious viral disease in poultry and wild birds, and it can cause significant economic loss worldwide. Eight viral strains were isolated by inoculating embryonated chicken eggs from the Poyang Lake region of China with swab samples. All eight of the NDV isolates were identified as class I genotype 3 strains, but they diverged notablely from class II viruses. Further analysis revealed that all eight NDV isolates were lentogenic strains containing the ¹¹²ERQER↓L¹¹⁷ motif at the F protein cleavage site. The strains were highly identical and were more species specific (chicken and waterfowl) than site specific (Nanchang and Duchang regions). The close phylogenetic proximity of these isolates indicates that viral transmission may happen between poultry and wild birds. Our study demonstrates that lentogenic class I NDVs exist in clinically healthy wild waterfowl and poultry within the Poyang Lake region. Active surveillance of these viruses to determine their evolution and origin is one of the most realistic strategies for preventing and controlling NDV outbreaks.     

Relationship between three novel SNPs of BRCA1 and canine mammary tumors

The BRCA1 gene plays an important role in the development of human breast cancer, and recent research indicated that genetic variations of BRCA1 are also related to canine mammary tumors (CMTs). Here, using rapid amplification of cDNA ends (RACE), we cloned the 5′- and 3′-UTRs of BRCA1. By direct sequencing of the flanking sequences of the 5′- and 3′-UTRs of BRCA1, three previously unreported single-nucleotide polymorphisms (SNPs) were identified, two (−1228T >C, −1173C >T) in the putative promoter regions and one non-synonymous SNP (63449G >A) in exon 23. Compared with 16 normal samples, the sequences from 34 CMTs suggested that SNP (−1173C >T) was associated with the development of CMTs (odds ratio (OR)=2.57, 95% confidence interval (CI): 1.07–6.15).     

农杆菌介导的朝鲜碱茅PuP5CS基因转化紫花苜蓿的研究

为提高紫花苜蓿(Medicago sativa)的抗逆性,利用在朝鲜碱茅(Puccinellia chinampoensis)已克隆的Pu P5CS基因成功构建了p CAMBIA3300-Pu P5CS表达载体,以子叶为外植体,通过农杆菌介导共培养法转化紫花苜蓿"公农5号"(M.sativa cv.Gongnong No.5),并以2.0 mg·L-1的草铵膦进行筛选,抗性愈伤组织诱导率为22.4%,经草铵膦筛选的植株进行PCR检测和RT-PCR检测。结果显示,共获得11株转化植株,表明Pu P5CS基因已转入T0紫花苜蓿植株,且能够在RNA水平上正常表达。     

Construction and Identification of PRRSV ORF5 Gene Prokaryotic Expression Vector

The present study was designed to construct recombinant plasmids,which could express porcine reproductive and respiratory syndrome virus (PRRSV) ORF5 gene.RNA was extracted from spleen and lung samples of the suspected pigs which were infected with PRRSV.According to PRRSV ORF5 gene,a pair of primers was designed for RT-PCR amplification.The ORF5 target gene was cloned into pMD19-T vector and then the recombinant pMD19-ORF5 was achieved.According to the sequencing results and the characteristics of expression vectors,a pair of primers with NcoⅠand XbaⅠenzyme cleavage sites was designed.Target fragment dORF5 was amplified and then connected to pProEXHTb and pNZ8149 vectors,respectively.And recombinant HTb-dORF5/DE3 and pNZ8149-dORF5/NZ3900 was induced by IPTG and Nisin,respectively,and analyzed by SDS-PAGE and Western blotting.Recombinant HTb-dORF5/DE3 induced by 1.5 mmol/L IPTG was expressed in the highest quantity.There were specific band at about 22 ku with reactionogenicity when it was tested by SDS-PAGE and Western blotting.Recombinant pNZ8149-dORF5/NZ3900 induced by 20 ng/mL Nisin was expressed in the highest quantity.There were specific band at about 19 ku with reactionogenicity when it was tested by SDS-PAGE and Western blotting.The IFA result showed specific green fluorescence.This study successfully constructed recombinant plasmids HTb-dORF5 and pNZ8149-dORF5 and expressed,the result laid a solid foundation for further development of PRRS vaccines.     

基于粪便纤维组织法探究异地驯养藏羚的食性

基于粪便纤维组织法探讨异地(铁卜加草原站)驯养藏羚的食性。结果表明,在基地生长的50种植物中,伊凡苔草、火绒草、川青早熟禾、洽草等是藏羚最喜爱食用植物,所占比例依次为18%、13%、11%和8%,其中伊凡苔草的茎、火绒草的叶、菥冥的果和迷果芹的花是其喜食部位。在基地驯养藏羚的食物组成中,各科所占比例依次为:禾本科(43%)莎草科(25%)菊科(14%)十字花科(7%)豆科(3%)。与可可西里地区类似,铁卜加地区藏羚以单子叶植物为主要食物。     

封育对伊犁绢蒿荒漠草地土壤养分的影响

以新疆伊犁绢蒿属荒漠草地为对象,研究了不同封育年限对草地土壤有机质、土壤全氮、全磷、全钾、碱解氮、速效磷、速效钾的影响。结果表明:随封育时间的延续,土壤有机质、全氮及碱解氮在表层基本呈先降后升趋势,且在封育9年均达到最高值;速效钾与速效磷均呈先降后升再降趋势,且在封育6年达到最大值;全钾与全磷无明显变化趋势。土壤有机质、全氮及速效养分呈现表聚现象,且土壤有机质与全量及速效养分相关性显著。