科尔沁沙地耐旱乡土灌木树种的选择及其生长比较

在干旱年份，从科尔沁沙地干燥沙丘选择特别耐旱的灌木树种进行育苗试验。通过对1、2年生苗木生长状况分析：叶底珠（Securinega suffruticosa）、鼠李（Rhamnus porflorus）、桃叶卫矛（Evonyms bungeanus）、驼绒蒿（Eurotia arborescens）和杠柳（Periplocasepium）等适应性强，1年生苗木高度为对照树种胡枝子的49．6％～101．8％，2年生苗木为对照树种胡枝子的89．0％～97．9％，可以进一步进行沙地造林试验；东北木蓼生长缓慢，不宜继续进行造林试验。     

东方田鼠肝脏裂解物筛选日本血吸虫成虫噬菌体展示cDNA文库

东方田鼠对血吸虫具有天然抗性。为寻找东方田鼠抗血吸虫抗性相关靶基因，探索其分子机理，我们将日本血吸虫童虫与东方田鼠不同组织／器官裂解物共培养，比较它们对血吸虫童虫的杀伤效果，之后，以杀伤效果较好的肝脏裂解物为探针，以亲和淘洗方法筛选日本血吸虫成虫（44d）噬菌体展示cDNA文库。经三轮筛选后，随机挑取92个噬菌体克隆进行序列测定及生物信息学分析，获得了19个有效EST序列，其中15条EST序列与已知基因或表达序列标签同源，4个EST序列与已知基因或表达序列标签均无同源性，为新的表达序列标签。对19个有效EST编码蛋白的功能预测结果显示，其中6个为卵壳蛋白（Eggshell protein）相关基因，10个EST及其编码蛋白的功能与血吸虫基因表达调控及蛋白质加工修饰相关。研究结果提示，东方田鼠直接或间接地影响血吸虫基因表达，进而影响血吸虫的发育，可能是东方田鼠具有抗血吸虫特性的一个重要分子机理。     

增产菌对复种燕麦产草量影响的试验

采用拌种、喷洒和拌种+喷洒3种方法,对临夏县北塬灌区麦后复种燕麦草增产效果进行了初步研究。结果 表明,增产菌对麦后复种燕麦的增产效果极显著(p<0.01),最佳拌种量300 g/hm2,最佳喷洒量300 g/hm2,最佳处 理组合为300 g/hm2拌种+300 g/hm2喷洒。     

Logistic方程在微生物分批培养动力学中的应用

将生态学领域中的Iogistic方程引入微生物动力学的研究中，建立了3个分别基于Monod方程、Herbert方程和Logistic方程的关于培养基中的微生物浓度(χ)和底物浓度(s)的模型以形成对照．同时建立了基于Logistic方程的关于微生物浓度(χ)和时间(t)的模型．这些均采用实验数据拟合以证明其精确性．该工作有助于研究微生物生长特性，同时有助于进一步地研究可生化降解的有机物在水体的微生物作用下的氧化分解过程，建立新的河流、湖泊、水库等生物学水质模型．本研究中采用的数值方法是非线性最小二乘法、Interior-reflective Newton算法．在Lorynebacterium glutamicum分批培养和Pseudomonas sp．分批培养中，基于Logistic方程的χ-s模型拟合的相关系数分别是0.9940和0．9867，在Pseudomonas sp．分批培养中，z-t模型拟合的相关系数是0．9972。     

银杏丰产栽培技术

银杏为我国传统的名贵树种,其生长缓慢、结实晚,一般要20多a才能开花结实,并且产量低。阐述银杏的生物学特征、繁殖方式、栽培管理方法、修剪整形及病虫害防治等实用技术,旨在为扩大银杏园林生产、提高银杏的种植质量和产量提供有益的参考。     

提高丘陵岗地楸树造林成活率的技术措施

以2 a生楸树实生苗为试验材料,研究了腐殖酸液肥、生物菌肥、保水剂和生根粉技术对提高丘陵山区困难立地造林成活率的应用效果.结果表明:在栽植穴内施入腐殖酸液肥、施用抗旱保水剂或使用GGR生根粉溶液对根系进行浸泡处理,均能显著提高楸树在困难立地上的造林成活率;在栽植穴内填加生物菌肥,对造林成活率没有显著影响.试验中,每株施用2.5 kg腐殖酸液肥或施用200 g抗旱保水剂,造林成活率最高;此外,使用1000 mg/L的GGR1号生根粉溶液浸泡苗木根系2 min,造林后成活率最高.     

安徽中南部地区水生蔬菜资源与生产考察

对安徽中南部14个县、市的水生蔬菜资源进行考察，共收集水生蔬菜资源样本62份，其中野生资源18份，地方品种44 份；收集到一些有特色的地方品种如雪湖贡藕、桐城水芹、绩溪水芋、庐江芹芽等，并对安徽中南部地区水生蔬菜种植生产 情况进行了考察。根据考察结果，提出目前我国栽培的水芹应有两个种，除水芹〔Oenanthe javanica(BL.)DC.〕外,另一个 种为中华水芹(Oenanthe sinensis Dunn）。     

温点旅游城市乡村旅游发展策略研究

以咸阳为例，对乡村旅游如何促进温点旅游城市的旅游发展作了探讨。     

生长素和细胞分裂素对平邑甜茶组培苗PAs和NO含量的影响

 以平邑甜茶继代组培苗为试材，研究了NAA、IBA、ZT和6-BA作用下组培苗的生长、内源多胺（PAs）和一氧化氮（NO）含量的变化。结果表明，在MS培养基中添加0.02 ~ 1.00 mg·L^-1的NAA或IBA后，外植体鲜样质量增加率和蛋白质含量以及多胺含量和一氧化氮含量均明显增加，其中浓度在0.10 ~ 0.50 mg·L^-1 时增加最高；同样浓度下，NAA处理的外植体鲜样质量增加率和腐胺含量高于IBA。在0.08 ~ 4.00 mg·L^-1范围内，随着ZT或6-BA浓度的升高，外植体鲜样质量增加率、可溶性蛋白含量、多胺含量和一氧化氮含量均逐渐升高；同样浓度下，6-BA处理的外植体鲜样质量增率和腐胺含量高于ZT。生长素和细胞分裂素促进组培苗生长与促进内源PAs和NO的生成相伴随，组培苗生长动态与内源多胺和一氧化氮含量的变化趋势一致。     

Relationship between endothelial dysfunction and renin-angiotensin-aldosterone system under the condition of fatigue stress and effect of herbs to dredge collaterals

AIM: To investigate the mechanisms and relationship among endothelial dysfunction, renin-angiotensin-aldosterone system (RAAS) and family of interleukins under the condition of excessive fatigue, by using the rats with fatigue stress. METHODS: Healthy male Wistar rats were randomly divided into control group, homocysteine (HCY) group, fatigue stress group, renshen group, shuangshen group and tongxinluo group. Radioimmunoassay was carried out to detect plasma renin activity (PRA), angiotensin II (AngⅡ), aldosterone (ALD), endothelin (ET), thromboxane-2 (TXA2), prostaglandin I2 (PGI2) in plasma and interleukin-1β, 2, 6, 10 (IL -1β, IL -2, IL -6) in sera. ELISA was used to detect NE and IL -10. The content of nitric oxide (NO) in sera was also detected. The bioinformatical analysis was used to determine the relationship between RAAS and endothelial dysfunction. RESULTS: Compared with control group, the levels of ET-1 and TXA2 in fatigue stress group were significantly increased (P<0.01, P<0.05), but the content of PGI2 and NO was significantly decreased (P<0.01, P<0.01). Compared with control group, the renin activity in plasma of animals in fatigue stress group was significantly decreased (P<0.01), the AngⅡ, IL -1β, IL -6 level was significantly increased compared with the control group (P<0.01, P<0.01), and was significantly increased compared with the HCY group (P<0.05, P<0.01, P<0.01). The NE level showed the tendency of decrease in different degree. After the intervention of three kinds of herbs to dredge collaterals, the ET-1, AngⅡ, IL -1β, IL-6 level in plasma was decreased in different degree (P<0.05, P<0.01, P<0.01), and at the same time, the contents of NO and NE level were significantly increased (P<0.05). The ALD level in tongxinluo groups was apparently higher than that in control group and the fatigue stress group (P<0.05). The bioinformatics analysis showed that Ang II and ET, IL-1; PGI2 and ALD; NO and ALD composed of three subsystems and interrelated according to the principle of optimality of complex system, and gradual change regulation was also observed in fatigue stress group. However, in control group, HCY group and tongxinluo group, the same interrelation among subsystems was not existed. CONCLUSION: In a state of long-term excessive fatigue, vascular endothelial dysfunction may be induced, and is related with renin-angiotensin-aldosterone system imbalance and serious turbulence of the autonomic dysfunction. Herbs to dredge collaterals could improve it significantly. The results suggest that bearing excessive fatigue and pressure in long-term may be the potential risk factors to induce vascular endothelial dysfunction and further result in cardio-cerebro vascular diseases, Tongluo therapy may be one of the useful ways to prevent such diseases.