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Mycobacteriosis due to infection of Mycobacterium marinum is a common disease in pond-cultured Chinese soft shell turtles, especially in those surviving beyond their first year. The infected turtles independently showed either heterophilic or histiocytic granulomas in various organs such as the spleen, liver, lungs, intestine, kidneys, stomach and pancreas. The heterophilic granuloma contained many acid-fast unbranching bacilli intracellularly in macrophages and extracellularly in the necrotic center. The histiocytic granuloma had only a few bacteria, mainly in the cytoplasm of Langhan's giant cells. The organisms were rarely observed in the advanced lesions of both types. Based on PCR assays for partial hsp65 gene of Mycobacterium spp., all of our strains were identified as M. marinum which can be divided into two groups. The strains of the first group induced heterophilic granulomas and had very high nucleotide sequence identities (99.8%-100%) to the reference strains of M. marinum (AF456471) and M. pseudoshottsii (AY550226). Those strains of the second group caused histiocytic granulomas and also showed very high identities (99.8%-100%) to the reference strains of M. marinum ATCC 927 (AF456470) and M. shottsii (AY550225). However, when we compared the partial sequence of the hsp65 gene from group one and two strains the identities between the two groups range from 98.8% to 99.3%, therefore we can not assert that these two belong to the same species. 相似文献
993.
1 我省河鲀鱼养殖和销售概况 我省的河鲀鱼养殖事业始于20世纪90年代初,经过十几年的发展,到目前为止,年间总产量已达到1 500t,占全国的50%以上. 相似文献
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C.M. Tu 《Soil biology & biochemistry》1982,14(1):57-61
Three soils, sandy loam, clay loam, and muck were subjected to different physical treatments, reinoculated with fresh soil and the effect of these treatments on the numbers of microorganisms and soil enzyme activities was studied. Soils were subjected to air-drying, freeze-drying, freezing, dry ice-freezing, autoclaving and oven drying. The results indicated that the microbial populations increased with some of the physical treatments after 2 or 7 days incubation, while, some of the treatments decreased the populations. Air-drying the clay loam inhibited urease and phosphatase activities. None of the treatments inhibited dehydrogenase activity in either the clay loam or the muck. However, a stimulatory effect after 4 days was evident in the muck with heat treatments. Heat treatments inhibited phosphatase activity in all soils and urease activity throughout the experiment in loams, whereas after 14 days, there was a rapid recovery of urease activity in the muck soil. Autoclaving resulted in a pronounced increase in C2H2 reduction in the three types of soils. Heating appears to have an effect in an organic soil where the formation of 2,3,5-triphenyltetrazolium formazan (formazan) and C2H4 were significantly increased. 相似文献
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提高大豆渣膳食纤维中可溶性成分的方法研究 总被引:2,自引:2,他引:2
以大豆渣为原料,采用微生物发酵、微射流均质机高压均质处理和高温蒸煮的方法来提高大豆膳食纤维中可溶性成分含量,研究不同发酵时间、不同处理压力和蒸煮温度以及蒸煮时间对提高豆渣可溶性膳食纤维(SDF)含量的影响。结果表明:利用发酵法可提高可溶性膳食纤维的含量达15%之多,并且在发酵的前6天,SDF含量增长很快,6 d后SDF的含量变化较少;而高压均质处理法提高可溶性膳食纤维含量的幅度在10%~28%之间,并随着处理压力的升高而增大;高温蒸煮法能提高可溶性膳食纤维的含量20%,121℃,20 min为较理想的蒸煮条件。 相似文献
1000.
运用实证研究、对比分析和相关分析等方法分析土地利用结构变化与碳收支的关系;探讨如何通过优化土地利用结构来实现碳收支的平衡。结果表明:净释碳量与各种土地利用类型的相关系数都很高,且与居民点及工矿用地、交通用地、水利设施用地、园地、林地成正相关,与耕地、牧草地、其它农用地、未利用地和其它土地成负相关。因此,增强碳汇功能必须优化森林结构,提高林地净初生产力,加强耕地保护力度,充分发挥耕地的碳汇作用;通过控制居民点及工矿用地规模来优化产业结构和产业布局,通过合理规划交通用地来优化能源利用率,以减弱碳排功能。 相似文献