搭架方式对杠板归药材产量与质量的影响

为探索杠板归规范化种植过程中搭架对其生育期、长势、产量及质量等的影响,对具有相同条件下的杠板归进行不搭架、搭散架、搭斜架、搭平架4种方式处理.观测记录不同处理方式的杠板归生育期和单株生长生物指标,检测不同处理方式的药材产量及有效成分.结果表明:不同搭架处理对杠板归生育期影响不明显,对药材槲皮素含量及产量有较大影响.杠板归药材种植中散架或平架为其理想的搭架方式.     

肾型传染性支气管炎病毒对鸡嘌呤代谢的影响研究

用T株肾型传染性支气管炎病毒(IBV)人工感染14日龄非免疫土种鸡建立感染模型,于攻毒前、攻毒后48 h、72 h、96 h、120 h 采集血样,测定血浆黄嘌呤氧化酶(xanthine oxidase, XOD)活性,探索鸡嘌呤代谢变化与肾型IBV致病机理之间的关系.结果表明,与攻毒前相比较, 攻毒后48 h和72 h血浆XOD活性明显增强, 差异显著(P<0.01,P<0.05).结果提示,XOD活性升高引起的嘌呤代谢紊乱可能是鸡肾型IB的病理机制之一.     

Effects of NK cells on the etiology of severe pre-eclampsia

AIM: To detect the effects of NK cells on the etiology of severe pre-eclampsia. METHODS: The peripheral blood and umbilical blood from severe pre-eclamptic patients and normal late pregnant women were collected. NK cells were seperated in percoll and counted by SAP. The proliferating ability of NK cells was measured by MTT and killing power of NK cells was determined by ［51Cr］ releasing test. The immunohistochemistry method was used to detect the quantity of NK cells and the expression of HLA-G in placenta of them. RESULTS: (1) Compared with that in normal late pregnant women, the counts of the NK cells in peripheral blood, umbilical blood and decidua of severe pre-eclamptic patients were significantly higher (all P<0.05). (2) Compared with that in normal late pregnant women, proliferating and killing ability of NK cells in peripheral blood and umbilical blood of severe pre-eclamptic patients were significantly higher. (3) The expression of HLA-G protein in placenta of severe pre-eclampsia group was lower than that in normal group (P<0.05). CONCLUSION: The quantity of NK cells in severe pre-eclamptic patients is increased with stronger biologic function. They may be related with the pathogenesis of severe pre-eclampsia.     

H5亚型禽流感病毒血凝素基因的克隆及表达

研究以RT-PCR方法从H5N1亚型禽流感病毒A/DKZJ/12/00(H5N1)中获得禽流感病毒HA部分基因,将目的基因定向克隆到原核表达载体pET-32a,将测序和酶切验证正确的阳性重组质粒转化大肠杆菌BL21,经IPTG诱导表达。结果表明该蛋白得到了可溶性的表达,表达蛋白的分子质量为25ku;Western-blot分析结果表明,该蛋白可以与禽流感H5阳性血清反应(禽流感的单克隆抗体所识别),检测HA的抗体时具有良好的免疫原性。     

H5N1亚型禽流感病毒神经氨酸酶基因的表达及纯化

用RT-PCR方法从H5N1亚型禽流感病毒（AIV）A／Duck／Zhejiang／12／00中获得NA基因，将目的基因定向克隆到原核表达载体pET-32a中，将序列测定和双酶切验证准确的阳性重组质粒转化大肠杆菌BL21，用IPTG诱导，经SDS-PAGE和Western-blot分析，结果显示，重组蛋白得到了可溶性表达，表达的蛋白质分子质量为33ku；该蛋白可以与禽流感H5N1亚型阳性血清反应，具有良好的免疫原性；ELISA检测结果表明，用此纯化蛋白作为包被抗原检测H5N1亚型AIV神经氨酸酶抗体具有良好的灵敏性。     

楼式高床羊舍对舍饲山羊的影响

在重庆市万州区研制、改造楼式羊舍,推广退耕还林地种草和舍养羊综合技术.并测定羊舍的小气候环境,舍养羊的草料消耗量、粪污排放量,比较研究山羊舍养羊与放牧饲养的生长、繁殖、疫病、养殖效益和对生态的影响,为三峡库区等地推广示范提供依据.     

禽流感病毒A/PFV/Restock/34(H7N1)HA基因在昆虫/杆状病毒系统中的表达

经RT-PCR扩增了禽流感病毒A/PFV／Restock／1／34（H7N1）1．7kbHA基因的cDNA，将其克隆到pMD18-T中并测序。在去除编码HA信号肽的核苷酸序列后，亚克隆到杆状病毒转移载体pBlueBacHis，筛选到重组质粒命名为pBlueBacHisH7HA，测序正确后与线性化的杆状病毒DNA（Bac-N-Blue^TMDNA）共转染sf9昆虫细胞，挑取蓝色蚀斑，经三轮蚀斑纯化，获得数株重组杆状病毒rpBlueHisH7HA。提取重组病毒DNA，经PCR证明目的基因片段已插入到杆状病毒基因组中，血凝实验、SDS-PAGE和Western blot实验结果表明HA基因在重组杆状病毒感染的HFive细胞中获得了表达。     

新城疫病毒Ⅰ系毒株通过Caspase途径诱导鸡胚成纤维细胞凋亡

应用不同Caspases抑制剂研究了新城疲病毒（NDV）Ⅰ系毒株诱导鸡胚成纤维细胞（CEF）凋亡的途径。结果，用20μmol／L的Z—VAD．fmk、Z—IETD．fmk和Z—LEHD．fmk分别处理CEF后，均能抑制NDVⅠ系毒株诱导的CEF凋亡；而用20μmol／L的Z—DEVD．fmk和Z—AEVD．fmk分别处理CEF,则不能抑制NDVⅠ系毒株诱导的CEF凋亡。由此表明，NDVⅠ系毒株能通过Caspase依赖性途径诱导CEF凋亡，其中Caspase-8和Caspase-9在凋亡中发挥重要作用，而Caspase-3和Caspase-10在凋亡中不发挥作用。     

大兴安岭高寒地区滑菇代料栽培技术

通过多年实践总结出一套适合本地的滑菇种植技术．包括菌种选择、培养料的制作、装袋、灭菌、接种、培养、出菇管理、采收全过程栽培技术．以期促进林区滑菇生产尽快发展．使大兴安岭地区成为滑菇夏季出菇基地．