国家经济安全审计实施路径研究

国家经济安全审计是指审计机关依赖有效的审计制度对被审单位财政、财务收支和经济活动的真实性、合法性和效益性进行审计的基础上，针对经济安全的关键领域，识别、评估、预警和报告危害经济安全的风险，确保财产权益安全完整的一种审计模式。该模式奠定了审计监督在国家经济安全体系中的基础性地位。创新审计模式及审计策略、变革国家审计体制、构建以审计监督为基础的新型国家经济安全体系是国家经济安全审计的三大实施路径。     

赣南林业可持续发展的探讨

可持续发展的理念是一种科学的发展观,注重经济发展的同时,也应注重生态环境和资源的可持续利用。在中国社会发展的过程中,林业建设发挥着重要的作用,对于生态环境和经济发展有着巨大的贡献。赣南是个山多地少的农业大市,保护和合理开发赣南的森林资源既能造福子孙后代,又能促进赣南经济社会协调发展。立足赣南本地的林业现状,阐述林业可持续发展对策。     

杨树抗黑斑病相关基因表达谱分析

利用黑斑病的高抗无性系美洲黑杨I-69及黑斑病的高感无性系欧美杨I-45为材料建立的2个cDNA文库,随机挑取cDNA克隆进行5'端EST序列测序,共获得有效序列20 023条.序列经聚类分析和拼接后,共获得10 816个Unigene,其中包括3 734个Contig,7 082个独立的Singleton.被注释的8 853个具有同源性匹配序列基因中,按照GO的分子功能、生物过程和细胞组分3个不同分类角度进行分类.在具有功能注释的8 853个Unigene中,选出330个与完成全基因组测序的毛果杨序列进行BLAST分析,结果发现有177个抗病相关候选基因出现在282个Unigene中,其中135个分布于杨树的18个不同连锁群上,其他42个基因位于还没定位的scaffolds上.所测定的这些EST序列为后期在基因组水平上研究杨树黑斑病的水平抗性遗传机制及进一步的相关基因发现奠定基础.

Abstract：

In an attempt to elucidate the molecular mechanism for resistance of black spot disease in poplar,gene expression profiles in leaves of Populus deltoides'Lus'(I-69/55)and P.euramericana'I-45/51',which were inoculated with the pathogen Marssonina brunnea f.sp.brunnea,were analyzed based on expressed sequence tags (ESTs).A total of 20 023 valid ESTs from the 5'terminal ends derived from corresponding cDNA libraries of the two poplar species were sequenced.Cluster analysis of the 20 023 sequences yielded 10 816 tentative unigenes,including 3 734 contigs and 7 082 singletons.All tentative unigenes were classified by Gene Ontology functional categories.To find resistance-associated candidate genes and locate them on poplar genome,330 unigenes was chosen from 8 853 annotated tentative unigenes,and their BLAST alignment was conducted with Populus trichocarpa assembly,1 77 related candidate resistant genes were found,and they presented in 282 unigenes.Among these genes,there were 1 35 genes located on 18 different linkage groups of poplar genome,and 42 genes located on the different scaffolds.This study supplied a resource of candidate genes for further exploring the genetic mechanism for the host horizontal resistance to the pathogen Marssonina brunnea at the whole genome range,and provided important information for further gene discovery.     

Preparation of Adjuvant Diluent for Live Vaccine against Classical Swine Fever and Evaluation of its Immune Effects

The aim of this study was to develop a novel oil-in-water (O/W) emulsion as adjuvant diluents (AD) for live vaccine against classical swine fever (CSF) that could effectively enhance the immune effect of vaccine.The AD was prepared by high-pressure homogenization technique.Formulations and preparation parameters were optimized with response surface design.Its stability, particle size, polydispersity (PDI) and Zeta potential were characterized.The humoral immune response and cellular immune response of the AD were evaluated with BALB/c mice by intramuscular injection.The particle size of the AD prepared by optimized formulation and parameters was 100.4 nm, PDI was 0.147, and Zeta potential was —28.7 mV.The experiment results showed that the AD had good stability.The AD was inoculated combined with live vaccine against CSF into BALB/c mice by intramuscular injection.The results showed that the live vaccine against CSF specific immune responses could be evoked in mice by co-inoculation with AD and vaccine.The cellular immune response levels in co-inoculated groups were significantly higher than control group (P<0.05), with obvious phenomena of higher levels of IFN-γ, IL-6 and IL-4 in serum.The result revealed that cellular immune capability significantly improved with the AD.The results strongly revealed that cellular immune capability significantly improved by introducing AD for effective immune-adjuvant for live vaccine against CSF.     

新媒体时代计算机专业就业指导课程教改探索

新媒体技术的广泛应用,对大学生尤其是计算机专业的大学生的思想行为和学习生活产生了深远的影响,在很大程度上拓展了教学空间,给计算机专业就业指导课程教学改革带来机遇。本文通过对计算机专业就业指导课程教学现状分析,把握新媒体技术带来的机遇,引入慕课教学和O2O模式教学,对新媒体时代计算机专业就业指导课程教学改革提出了可行的建议。     

水稻OsZ314基因克隆及转录激活验证

穗是水稻(Oryza sativa L.)重要的生殖器官,其生长发育直接影响水稻的产量和品质。前期研究中,我们对水稻生殖生长时期穗发育各阶段的转录组、蛋白组数据进行联合分析,筛选到一个MYB基因家族的转录因子OsZ314基因。生物学信息分析表明,该基因位于1号染色体上,拥有典型的R2R3-MYB型转录因子结构域,且在水稻幼穗时期表达量最高;亚细胞定位结果显示,OsZ314基因定位于细胞核中;转录激活试验表明,OsZ314基因具有转录激活功能。本研究为深入探索OsZ314基因在水稻穗发育时期的分子生物学功能奠定了良好的基础。     

猪链球菌病和副猪嗜血杆菌病鉴别诊断与防治方案

猪链球菌病和副猪嗜血杆菌病是由猪链球菌和副猪嗜血杆菌这两类猪场常在菌引起的。这两类细菌会对各个年龄阶段的猪群易感,尤其以仔猪最易感。同时,它们给养殖业临床生产造成了巨大的困扰并引起巨大的经济损失。这两类疾病所引起的临床症状及其相似,会造成临床治疗时因鉴别错误而耽误最佳的治疗时间。文章旨在通过临床诊断以及解剖观察的病理变化来区分两种疾病,并提供防治方案。     

草莓白化相关病毒中国分离物全基因组分析

草莓白化相关病毒(strawberrypallidosis-associatedvirus,SPa V)属于长线形病毒科(Closteroviridae)毛形病毒属(Crinivirus),可引起草莓病害,2017年在中国首次报道。采用高通量测序、RACE和RT-PCR技术获得了SPa V中国分离物(FJ)的基因组全长。该病毒含有两条正单链基因组RNA1和RNA2。RNA1全长8 048 nt,5′和3′非编码区序列分别为264和197 nt,含有3个开放阅读框(ORF),分别编码ORF 1a/1b融合蛋白和p9蛋白。RNA2全长7 977 nt,5′和3′非编码区序列分别为248和186 nt,含有8个开放阅读框(ORF),分别编码HSP70h、CPh、CP、CPm、p7、p6、p9和p28等8个蛋白。RNA1和RNA2与美国M1分离物分别具有98.5%和99.0%的核苷酸一致性;系统发育分析结果表明,SPa V中国分离物(FJ)单独处在一个分支。对SPa V来源的小RNA的分析表明,来源于SPa V的小RAN长度以21和22 nt为主。     

公众参与城市生活垃圾管理的影响因素研究

基于公众视角,研究影响公众参与城市生活垃圾管理的因素及影响程度。通过设计调查问卷获得相关数据,利用主成分分析和回归分析方法对环境保护需求、法律制度保障等因素影响公众参与城市生活垃圾管理的程度进行实证研究。研究结果表明:相较于权利的追求、公众的利益追求、个人修养这些内部因素,环境保护需求、法律制度保障、环保组织推动这些外部因素对公众参与城市生活垃圾管理的推动作用要小。而社会监督力量和信息技术发展对公众参与城市生活垃圾管理的推动作用则表现不明显。研究可为城市市政相关部门制定政策提供指导和借鉴。