脂肪型脂肪酸结合蛋白的生理功能及在禽类中的研究进展

脂肪型脂肪酸结合蛋白(A-FABP)又称为脂肪酸结合蛋白4(FABP4)或A-FABP,是脂肪酸结合蛋白(FABPs)家族中的一员,能结合多种疏水性化合物,在脂肪和卵巢组织中分布广泛,参与脂肪酸的合成代谢,作用于卵巢组织中,对于禽类产蛋中脂肪酸的沉积会起到关键的作用,调控禽类产蛋过程中蛋的脂肪酸营养沉积,提高产蛋优良性能。该蛋白又与肌间脂肪(IMF)含量有关,保证了肉质的良好品质,这让AFABP在禽类养殖中的研究逐渐成为热点。论文就该蛋白的生理功能和在禽类中的研究进展做一介绍。     

对液压传动车辆油液的正确选择及使用

对液压油的分类及物理、化学性能及污染物类型做了简单介绍,主要对液压油的正确选择和使用提出了要求。     

陆良县中枢镇农业增收途径的探索

从中枢镇农业产业结构现状和存在的问题人手，经认真分析得出中枢镇农业产业结构现状优势大，结构趋于基本合理的结论，但也存在许多问题和困难，基于这样的原因，作者认为需要在现在的基础上重新认真的定位，使中枢镇的农业产业化做优、做强、做大。     

Evaluation of Joint Toxicity of Enrofloxacin and Three Antimicrobials on MRC-5 Cells

The aim of this study was to evaluate the combined toxicity of enrofloxacin with each of three antimicrobials (ciprofloxacin,florfenicol and sulfamethazine).MRC-5 cells were used as a cell model to simulate the damage of lung cells caused by mixed contamination of antimicrobials.Multiple concentration gradients and mixing ratios were set.CCK-8 method was used to determine the inhibition rate of cell growth caused by four antimicrobials and the inhibition rate of cell growth caused by enofloxacin mixed with three antimicrobials,respectively.Then Chou-Talalay method was used to fit the median effect plot and to calculate the combination index (CI) value.The results showed that the growth inhibition rates of MCR-5 cells caused by four single drugs went up in a step-like manner with the increase of drug concentration in the tested concentration range,among them,the growth inhibitory rate of MCR-5 cells by florfenicol was low (<4.5%).The combined toxicity of the three binary combinations showed a concentration-dependent and mixing-ratio dependence.Mixing enrofloxacin and ciprofloxacin showed synergistic toxicity (CI<1) on MRC-5 cells at high and middle concentration groups,and antagonistic toxicity (CI>1) at the very-low-concentration groups.Mixing ciprofloxacin and florfenicol mainly showed an inhibited toxicity (CI>1).Binary combination enrofloxacin and sulfamethazine might present either a synergistic joint toxicity (CI<1) or an antagonistic joint toxicity (CI>1) as the concentration and mixing ratio changing.This study showed that it was necessary to assess the combined toxicity of antimicrobials in the toxicity evaluation of antimicrobials.Using Chou-Talalay method,the joint toxicity of multiple antimicrobials could be quickly and efficiently determined at cellular level.     

洞庭湖区双季超级杂交稻栽培示范

湘阴县2008年示范种植696．7hm^2双季超级杂交稻，取得了显著的增产、增收效果，详细介绍了品种搭配、浸种催芽、播期安排、培育壮秧、大田耕整、施肥、灌溉、病虫防治等配套栽培技术以及存在的问题，并提出了建议。     

银染mRNA差异显示方法在致突变实验中的初步应用

为建立在药物致突变研究中应用银染 m RNA差异显示的方法 ,试验提取未经过 /经过环磷酰胺处理的 Balb/c小鼠肝组织的总 RNA ,并以此为模板 ,采用 d T1 2 CG、d T1 2 AG、d T1 2 GG为锚定引物 ,通过反转录、差异显示 PCR反应 ,以 6 %变性聚丙烯酰胺凝胶电泳分离差异条带 ,回收后将其再扩增。结果表明 ,RNA投入量为 3μg、镁离子浓度为 1.5～ 2 .0 mm ol/L、退火温度为 4 0℃时 ,扩增的片段条带清晰、背景低 ,差异条带明显 ,再扩增条带单一。银染 m RNA差异显示技术可成功应用于药物致突变的研究。     

Islet cells modified with PD-L1-GPI molecule inhibit attack of reactive T cells

AIM: To investigate the immune depressive effect on the reactive T cells and to explore the immunologic injury mechanism of beta cells of islet in type 1 diabetes mellitus (DM-1). METHODS: pAd5/ PD-L1-GPI adenovirus vector with target gene was constructed and transfected into NIT cells which are known as a mouse insuloma cell line. The highly expressed membrane protein of PD-L1-GPI was confirmed by Western blotting. The peripheral blood non-adherence lymph leukocytes and target cells were cultured to detect lymph leukocyte proliferation and the T cell function. The level of IL-2, TNF-α and IFN-γ were detected in the cell culture fluid. RESULTS: Compared with the control group, the NIT cells modified with PD-L1-GPI inhibited the sensitized lymph leukocyte proliferation effectively and down-regulated the level of some cytokine secretions such as IL-2, IFN-γ and TNF-α (P<0.05). CONCLUSION: The islet cells expressed the PD-L1 gene inhibit the reactive responsive T cells, block up the cytotoxic effect of autoimmunity T cells, and induce the immunotolerance, which would be a value direction of the therapy of DM.     

褐色凝固型酸奶的制备工艺研究

褐色酸奶是以牛乳和葡萄糖为主要原料,通过加热产生美拉德反应,对褐变后的牛乳进行接种和发酵,得到褐色酸奶制品。本研究以感官得分和乳清析出率为指标,通过单因素试验及正交试验,研究并确定了褐色酸奶的加工工艺参数。试验结果表明,褐色酸奶的最佳加工工艺参数为葡萄糖添加量6%,褐变温度95 ℃,褐变时间5 h,发酵剂接种量5%,果胶添加量0.2%,发酵时间6 h,发酵温度42 ℃。在此条件下得到的褐色酸奶感官评定分数为93分,乳清析出率为5.3%。     

四种杀虫剂对橡胶盔蚧的防效试验

2004年8月进行4种杀虫剂对橡胶盔蚧的防效试验,结果表明40%杀扑磷乳油800倍液、2.5%杀蚧乳油 800 倍液、2.5% 蛛八蚧乳油 800～1　000 倍液、40% 氧化乐果乳油 800 倍液对橡胶盔蚧 1、2 龄若虫效果显著,14d 的防效均在91.3%以上;4种药剂的 800倍液对3龄若虫、成虫的试验结果,40%杀扑磷施药后 14d 防效为 64.7%,对成虫无效;对橡胶树无药害。     

乌药种子性状产地表型变异研究

对我国乌药自然分布区14个产地采集的乌药种子进行取样观测,分别测定其种子的长、宽、重,并进行播种试验,观测种子的出苗率;分析了乌药种子形态的产地差异、地理变异规律及种子形态对种子出苗率和苗木生长的影响.分析表明:乌药种子形态存在显著的产地差异,种子的大小存在一定的经向变异,种形指数(宽)与经度呈显著的负相关.种形指数与苗期生长有密切的关系.