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Antigens in the extracellular protein (ECP) complexes of Bacteroides nodosus, isolated from sheep with either benign or virulent footrot, were studied by immunoelectrophoresis (IEP). Rabbit antisera against ECP from virulent and benign strains, were used in homologous and heterologous crossed IEP. Four precipitin peaks unique to the virulent strain, and five peaks unique to the benign strain were identified. In an attempt to characterize the different antigens in ECP, rabbit antisera were raised against an outer membrane protein (OMP, mol. wt. 35 000 daltons), pili and various proteases of virulent and benign strains of B. nodosus. No precipitin band was observed when ECP from both B. nodosus strains were reacted against anti-OMP and anti-pilus antisera. However, single precipitin bands unique to one protease from the benign strain and one protease from the virulent strain were identified. The results suggest that specific antigens other than proteases or pili are important in determining whether a B. nodosus isolate is virulent or benign. 相似文献
115.
Isolated dog neutrophils exposed to known chemotactic factors assumed a bipolar configuration in suspension. This response was initiated within 2 minutes with a gradual return to spherical shape. The rate of return to sphere was positively correlated with the dose of stimulant. Dog neutrophils exposed to N-formylmethionyl-leucyl-phenylalanine (fMLP) did not adapt to bipolar conformation and did not bind radiolabeled fMLP. These findings are consistent with other species (pig and cow) that also did not respond to fMLP. 相似文献
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C A Osborne T D O'Brien H K Ghobrial L Meihak J B Stevens 《Veterinary Clinics of North America: Small Animal Practice》1986,16(1):45-65
Crystalluria results from oversaturation of urine with crystallogenic substance. However, oversaturation may occur as a result of in vitro as well as in vivo events. The microscopic appearance of crystals only represents a tentative identification of their composition because variable conditions associated with their formation, growth, and dissolution may alter their appearance. Definitive identification is dependent on physical methods such as optical crystallography, x-ray diffraction, and electron microscopic analysis. 相似文献
118.
齐卡商品肉兔在不同饲养条件下的育肥效果 总被引:1,自引:0,他引:1
作者在不同条件下,采用全窝育肥法,重复两年、两个季节测定了齐卡商品肉兔的育肥效果;探讨了温度、季节、营养水平、育肥始重对育肥效果的影响。结果表明:在试验场育肥250只齐卡商品兔,90日龄育肥重2.33~2.53公斤,日均增重31克以上,耗料指数3.31:1,育肥成活率95%以上,屠宰率52%,净肉率80%,肉骨比5.35:1,兔肉品质正常。在农村条件下育肥180只,90~100日龄育肥重2.2~2.4公斤,耗精料指数2.55:1,育肥成活率88%~93%。饲养齐卡商品兔与饲养二元杂交兔相比,每只可增加经济收入20%以上。育肥季节和始重明显影响育肥未重(P<0.05)。依据本试验,作者建议齐卡商品兔适宜35日龄断奶,育肥始重应不低于600克;寒冷季节育肥,育肥第一月的室温应不低于10℃。育肥适宜营养水平建议消化能(DE)2600~2700大卡/公斤,粗蛋白(CP)18%,粗纤维(CF)12%以上。 相似文献
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将构建的pBST2~6工程菌质粒用限制性内切酶BamHI/BglⅡ酶切,经琼脂糖凝胶电泳和电洗脱,回收147bp的目的ST1基因。随之将该基因分别重组到能有效表达K99菌毛抗原和LacZ酶的pGK99之K99基因BglⅡ位点和pUC18的BamHI位点中。通过ST1基因探针菌落原位杂交、特定酶切分析及DNA序列分析,筛选并鉴定出了理想重组子,从而构建出了能分别表达ST1融合基因产物的工程菌株pSK219和pXST1。 相似文献
120.
Twelve nonlactating dairy cows, free of signs of liver disease and with normal serum activities of liver-derived enzymes and normal liver biopsy tissue, were examined over a 72-hour period for serum total bile acid concentrations. The cattle were fed hay twice daily, and blood samples were obtained every hour for 24 hours, every other hour for 24 hours, then every hour for 24 hours. After 3 weeks, the study was repeated on 6 of the cattle, thus providing data for eighteen 72-hour periods. Serum bile acid concentration varied greatly over the 72 hours, with the range being from one third to 3 times the median. There were variations by as much as 60 mumol/L from 1 hour to the next. After another 3 weeks, 8 of the cattle were deprived of hay for 48 hours and then fed hay morning and afternoon of the third (last) day of the study. There was no significant reduction in bile acid concentration after withholding the hay, but the variability was reduced (P = 0.02) during the last 20 hours of the hay-deprivation period. In 3 ancillary studies, serum bile acid concentrations were examined over a 48-hour period in 2 cows in early lactation, 3 cows in midlactation, and two 6-month-old heifers. The cows were fed hay and grain twice daily, and the heifers were fed only hay twice daily. In comparison with values for the 12 nonlactating cows fed hay twice daily, mean serum bile acid concentration in the recently freshened cows was significantly (P < 0.002) higher (62.9 vs 22.0 mumol/L).(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献